Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,102,999	C→G	5.1%	noncoding (35/76 nt)	PP_t23 →	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,102,999	0	C	G	5.1%	252.9 / 2.9	79	noncoding (35/76 nt)	PP_t23	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (30/45);  new base G (2/2);  total (32/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TCATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGT  >  NC_002947/2102855‑2103145                                                                                                                                                 |                                                                                                                                                   tCATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTCCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                                                                >  7:251915/1‑149 (MQ=255)      ttCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                                                                           <  3:150127/149‑1 (MQ=255)      ttCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                                                                           <  7:209718/149‑1 (MQ=255)        cTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCTTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                                                                                                                                         >  6:388530/1‑149 (MQ=255)           gTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGACCCCGACCTTGGCAAGGTCgtgctc                                                                                                                                       <  6:277051/148‑6 (MQ=255)            tGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                                                                                                                     >  1:343788/1‑149 (MQ=255)              cACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCCTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                                                                                                                     >  8:15930/1‑147 (MQ=255)               aCTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                                                                          <  7:282687/141‑3 (MQ=255)               aCTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                                                                          >  8:282687/1‑139 (MQ=255)                 tGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                                                                >  4:45788/1‑149 (MQ=255)                     aaGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAg                                                                                                                            >  8:159526/1‑149 (MQ=255)                       gtgtTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtc                                                                                                                          <  4:160871/149‑1 (MQ=40)                         gttgtGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc                                                                                                                        <  3:249171/149‑1 (MQ=38)                             tgtgtCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt                                                                                                                     <  2:281034/148‑1 (MQ=35)                             tgtgtCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt                                                                                                                     <  3:342823/148‑1 (MQ=35)                               tgtCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                                                                                       <  2:367831/144‑1 (MQ=34)                               tgtCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                                                                                       >  1:367831/1‑144 (MQ=34)                                 tCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                                                 <  3:45788/148‑1 (MQ=34)                                   cccTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                                                                  <  4:343849/145‑1 (MQ=34)                                   cccTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                                                                  >  3:343849/1‑145 (MQ=34)                                    ccTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                                                         >  4:68584/1‑137 (MQ=34)                                    ccTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                                                         <  3:68584/137‑1 (MQ=34)                                     cTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                                                                   <  3:202427/126‑1 (MQ=35)                                     cTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                                                                   >  4:202427/1‑126 (MQ=35)                                         gTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGt                                                                                                         <  8:231357/148‑1 (MQ=34)                                              gTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAAt                                                                                                   <  2:343788/149‑1 (MQ=38)                                                          acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc                                                                                                                                             <  2:324366/95‑1 (MQ=37)                                                             gCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATg                                                                                    <  2:285135/149‑1 (MQ=255)                                                               ccTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCg                                                                                  <  5:94515/149‑1 (MQ=255)                                                               ccTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCg                                                                                  <  3:168938/149‑1 (MQ=255)                                                                   tCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTg                                                                               >  3:325740/1‑148 (MQ=255)                                                                   aaaggtaggTGACAGGGGTTCGAGTACCCTAGTGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGGCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTg                                                                               <  3:201032/141‑1 (MQ=17)                                                                   aaagggcggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTg                                                                               <  3:218264/144‑1 (MQ=255)                                                                     aCGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCGCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCGCCAGTTGAAATACTGTGGCGTCTATGCGGTGCAg                                                                            >  5:193989/1‑149 (MQ=255)                                                                     aCGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCa                                                                             <  7:159526/148‑1 (MQ=255)                                                                       ggcggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtg                                                                          >  8:62106/1‑149 (MQ=255)                                                                          ggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggtg                                                                       <  4:325740/149‑1 (MQ=255)                                                                              aCAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggtggtg                                                                    <  7:339394/148‑1 (MQ=255)                                                                               cAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGTAGTGGTGGTGaa                                                                  <  5:388530/149‑1 (MQ=255)                                                                               cAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggtggtg                                                                    >  5:330040/1‑147 (MQ=255)                                                                                aGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGa                                                                   >  6:210067/1‑147 (MQ=255)                                                                                aGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGa                                                                   <  5:210067/147‑1 (MQ=255)                                                                                aGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAg                                                                 <  8:251915/149‑1 (MQ=255)                                                                                aGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAg                                                                 <  7:15930/149‑1 (MQ=255)                                                                                   ggTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTc                                                              <  2:283734/149‑1 (MQ=255)                                                                                              ccTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCt                                                   >  7:178538/1‑149 (MQ=255)                                                                                              ccTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCAAGTTTAAATACTGTGGCGTCTATGCGGGGCAGTGGTGGTGAAGGTCGTaaa                                                         >  7:328713/1‑143 (MQ=255)                                                                                              ccTAGGGGACGCCAAAAGCGGGTATAGCTCAGTGGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGGCGTaaa                                                         <  8:328713/143‑1 (MQ=255)                                                                                                   gggACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggt                                                                        <  3:110688/123‑1 (MQ=255)                                                                                                   gggACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggt                                                                        >  4:110688/1‑123 (MQ=255)                                                                                                           aaaTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACg                                              >  4:197591/1‑141 (MQ=255)                                                                                                           aaaTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACg                                              <  3:197591/141‑1 (MQ=255)                                                                                                            aaTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTg                                     <  6:330040/149‑1 (MQ=255)                                                                                                                 gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGc                                                                                           <  8:306527/90‑1 (MQ=255)                                                                                                                 gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGc                                                                                           >  7:306527/1‑90 (MQ=255)                                                                                                                        gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACg                                       >  8:68549/1‑135 (MQ=255)                                                                                                                        gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACg                                       <  7:68549/135‑1 (MQ=255)                                                                                                                          tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAAt                       >  8:252430/1‑149 (MQ=255)                                                                                                                          tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAAt                       >  5:151134/1‑149 (MQ=255)                                                                                                                          tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAAt                       >  3:200614/1‑149 (MQ=255)                                                                                                                            aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAAtt                      <  7:62106/148‑1 (MQ=255)                                                                                                                            aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTg                     >  8:171112/1‑149 (MQ=255)                                                                                                                              ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCg                   >  1:129094/1‑149 (MQ=255)                                                                                                                              ttGGTAGAGCACGACCTTGCCAAGGTAGGGGTCTCGAGTTAGAGTCTCGTTTCCCGCTACAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGCCGTAAAGGCGCTTAACGACGAACGTGGTAAAGCTTCCAATTGCg                   >  6:215226/1‑149 (MQ=37)                                                                                                                               tGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCg                   <  7:171112/148‑1 (MQ=255)                                                                                                                                ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCgg                  <  8:178538/148‑1 (MQ=255)                                                                                                                                ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCgg                  <  7:252430/148‑1 (MQ=255)                                                                                                                                  tAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCgg                  >  3:297146/1‑146 (MQ=255)                                                                                                                                  tAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCgg                  <  4:297146/146‑1 (MQ=255)                                                                                                                                   agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAg                               <  1:90246/132‑1 (MQ=255)                                                                                                                                   agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAg                               >  2:90246/1‑132 (MQ=255)                                                                                                                                      gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGc           <  6:381922/149‑1 (MQ=255)                                                                                                                                       cccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCt          >  3:344936/3‑149 (MQ=255)                                                                                                                                        aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTc         <  2:129094/149‑1 (MQ=255)                                                                                                                                         cGACCTTGCCAAGGTCGTGGTCGCGAGTTCGAGTCTCGTTTCCCGCTACAGTTTAAATACTGTGGCGTCTATGCTGTGCAGTGGTGGTGAAGGCCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCa        <  5:215226/149‑1 (MQ=255)                                                                                                                                          gACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAgg                                                       <  4:210021/101‑1 (MQ=255)                                                                                                                                          gACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAgg                                                       >  3:210021/1‑101 (MQ=255)                                                                                                                                            ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGtt     <  4:200614/149‑1 (MQ=255)                                                                                                                                                gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGt  <  6:151134/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TCATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGT  >  NC_002947/2102855‑2103145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑