BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,112,035	T→C	5.8%	N751D (AAC→GAC) ‡	PP_1882 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,112,035	0	T	C	5.8%	313.2 / 6.3	103	N751D (AAC→GAC) ‡	PP_1882	membrane protein
Reads supporting (aligned to +/- strand): ref base T (49/48); new base C (4/2); total (53/50)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 104 total aligned reads displayed.

CCAGGTCTCGGTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCTCTCCTGTATCCG  >  NC_002947/2111892‑2112182

ccAGGTCTCGGTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGa                                                                                                                                                 >  2:113930/1‑149 (MQ=255)

  aGGTCTCGGTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCAACGCCGATc                                                                                                                                               <  6:120040/149‑1 (MQ=255)

    gTCTCGGTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCTCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCgg                                                                                                                                             <  8:10592/149‑1 (MQ=255)

    gTCTCGGTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCgg                                                                                                                                             >  3:113349/1‑149 (MQ=255)

      ctcGGTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGt                                                                                                                                           >  5:307408/1‑149 (MQ=255)

         ggTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCTTGGTGATCGGCGTTGCCGATCGGGTc                                                                                                                                          <  7:172414/147‑1 (MQ=255)

         ggTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGGTCTGCACCTGGGGGCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTc                                                                                                                                          <  3:248283/147‑1 (MQ=255)

          gTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAg                                                                                                                                       <  6:307408/149‑1 (MQ=255)

           tCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGt                                                                                                                                      <  8:31209/149‑1 (MQ=255)

               gCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGt                                                                                                                                  >  8:199110/1‑149 (MQ=255)

               gCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGt                                                                                                                                  >  6:344674/1‑149 (MQ=255)

                 cACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTtg                                                                                                                                >  3:347875/1‑149 (MQ=255)

                  aCTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTtgt                                                                                                                               >  5:123366/1‑149 (MQ=255)

                       tGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGtgctgc                                                                                                                          <  7:242804/149‑1 (MQ=255)

                        ggCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTgctgct                                                                                                                         >  4:291150/1‑149 (MQ=255)

                         gCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGAt                                                                                                                                                >  2:300301/1‑125 (MQ=255)

                         gCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGAt                                                                                                                                                <  1:300301/125‑1 (MQ=255)

                         gCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTc                                                                                                                        >  5:127937/1‑149 (MQ=255)

                           ccGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCa                                                                                                                      >  2:352082/1‑149 (MQ=255)

                              tGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCAcgt                                                                                                                   <  1:262466/149‑1 (MQ=255)

                                gcTGGGGGGTGGATGGGAGGTTCTTGCGGTTGCGGTTCTGGTCCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGGTCCAcgtc                                                                                                                  <  4:64007/147‑1 (MQ=255)

                                ccTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCAcgtc                                                                                                                  <  6:127937/148‑1 (MQ=255)

                                    gCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACgtcgtc                                                                                                               <  1:193527/147‑1 (MQ=255)

                                      gggTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCggg                                                                                                           <  5:155351/149‑1 (MQ=255)

                                      gggTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCggg                                                                                                           <  4:113349/149‑1 (MQ=255)

                                        gTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTg                                                                                                                                                     >  2:205366/1‑105 (MQ=255)

                                        gTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTg                                                                                                                                                     <  1:205366/105‑1 (MQ=255)

                                        gTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTtgt                                                                                                                               <  4:87536/127‑1 (MQ=255)

                                        gTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTtgt                                                                                                                               >  3:87536/1‑127 (MQ=255)

                                           gATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGa                                                                                                       <  7:258280/148‑1 (MQ=255)

                                            aTCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATg                                                                                                     >  4:198991/1‑149 (MQ=255)

                                            aTCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATg                                                                                                     <  2:365438/149‑1 (MQ=255)

                                             tCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATgg                                                                                                    >  8:93051/1‑149 (MQ=255)

                                              cGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGt                                                                                                   >  5:276057/1‑149 (MQ=255)

                                               ggACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGt                                                                                                   <  6:113941/148‑1 (MQ=255)

                                                    ttCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTgcagca                                                                                             <  5:344674/149‑1 (MQ=255)

                                                      cATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCAtt                                                                                           >  4:347842/1‑149 (MQ=255)

                                                                 ggTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGTCCTGGTGATCGGCAACGCCGATcacca                                                                                                                                           <  3:97065/90‑5 (MQ=255)

                                                                 ggTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGTCCTGGTGATCGGCAACGCCGATcacca                                                                                                                                           >  4:97065/1‑86 (MQ=255)

                                                                 ggTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCAcgtcg                                                                                                                 >  7:320926/1‑116 (MQ=255)

                                                                 ggTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCAcgtcg                                                                                                                 <  8:320926/116‑1 (MQ=255)

                                                                  gTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTg                                                                                                  >  4:184644/1‑130 (MQ=255)

                                                                  gTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTg                                                                                                  <  3:184644/130‑1 (MQ=255)

                                                                   ttCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAg‑tc                                                                             <  7:199110/149‑1 (MQ=255)

                                                                      tGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAg‑tcgtc                                                                          >  5:163768/1‑149 (MQ=255)

                                                                        cACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGc                                                                                                                                                    <  8:14800/74‑1 (MQ=255)

                                                                        cACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGc                                                                                     <  3:265062/137‑1 (MQ=255)

                                                                        cACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGc                                                                                     >  4:265062/1‑137 (MQ=255)

                                                                        cACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAg‑tcgtc                                                                          <  6:123366/147‑1 (MQ=255)

                                                                         aCCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTgcagcgt                                                                                            >  6:277093/1‑127 (MQ=255)

                                                                         aCCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTa                                                                       <  6:276057/149‑1 (MQ=255)

                                                                         aCCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTa                                                                       >  8:171621/1‑149 (MQ=255)

                                                                                gTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCAATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCCCGtt                                                                >  6:325275/1‑149 (MQ=255)

                                                                                     gATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGt                                                           >  4:342852/1‑149 (MQ=255)

                                                                                     gATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGt                                                           >  2:252746/1‑149 (MQ=255)

                                                                                      aTTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCTTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACgtcgtc                                                                                                               <  6:94205/97‑1 (MQ=255)

                                                                                      aTTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCATTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACgtcgtc                                                                                                               >  5:94205/1‑97 (MQ=255)

                                                                                       tttCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTa                                                         >  6:139536/1‑149 (MQ=255)

                                                                                        ttCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGt                                                           <  8:46753/146‑1 (MQ=255)

                                                                                        ttCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGt                                                           >  7:46753/1‑146 (MQ=255)

                                                                                               tcCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGtgc                                                                                                                             <  7:303681/74‑1 (MQ=255)

                                                                                               tcCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGtgc                                                                                                                             >  8:303681/1‑74 (MQ=255)

                                                                                               tcCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTg                                                  >  2:271682/1‑148 (MQ=255)

                                                                                               tcCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTg                                                  >  3:306464/1‑148 (MQ=255)

                                                                                                cccGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTg                                                  >  8:88677/1‑147 (MQ=255)

                                                                                                 ccGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAg                                               <  7:93051/149‑1 (MQ=255)

                                                                                                  cGCCTTCTCGCCAGCTCTAGCGAATGGCGCCCTGGTGCTCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAgg                                              <  8:176307/149‑1 (MQ=255)

                                                                                                     cTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTAc                                           <  3:291150/149‑1 (MQ=255)

                                                                                                         tcGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGc                                                                                         <  7:97290/100‑1 (MQ=255)

                                                                                                         tcGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGttttt                                                             >  6:133389/1‑127 (MQ=255)

                                                                                                         tcGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTActgct                                       >  2:284895/1‑149 (MQ=255)

                                                                                                           gCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTgg                                     <  3:347842/149‑1 (MQ=255)

                                                                                                           gCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTgg                                     <  4:187221/149‑1 (MQ=255)

                                                                                                             cAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGc                                   <  6:163768/149‑1 (MQ=255)

                                                                                                              aGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCt                                  <  1:113930/149‑1 (MQ=255)

                                                                                                              aGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCt                                  <  2:375791/149‑1 (MQ=255)

                                                                                                               gCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCtt                                 <  1:271682/149‑1 (MQ=255)

                                                                                                                   tAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGc                               <  5:139536/147‑1 (MQ=255)

                                                                                                                   tAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAg                             >  1:133544/1‑149 (MQ=255)

                                                                                                                     gCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAGCTCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGc                            <  1:284895/149‑1 (MQ=255)

                                                                                                                     gCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGc                            <  1:352082/148‑1 (MQ=255)

                                                                                                                        aaTGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGt                        <  3:154218/149‑1 (MQ=255)

                                                                                                                           ggCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGc                                   <  7:167988/135‑1 (MQ=255)

                                                                                                                           ggCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGc                                   >  8:167988/1‑135 (MQ=255)

                                                                                                                            gcgcCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAg                                               <  4:67925/122‑1 (MQ=255)

                                                                                                                            gcgcCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAg                                               >  3:67925/1‑122 (MQ=255)

                                                                                                                                   ggTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGctc             >  2:207397/1‑149 (MQ=255)

                                                                                                                                    gTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGctct            >  6:121023/1‑149 (MQ=255)

                                                                                                                                    ccgATCGGCAACGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTAGCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGctct            >  8:127072/3‑149 (MQ=255)

                                                                                                                                    ccgATCGGCAACGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGctct            >  2:312741/3‑149 (MQ=255)

                                                                                                                                    ccgATCGGCAACGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGctct            >  8:65222/3‑149 (MQ=255)

                                                                                                                                     tGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCtctc           >  5:136511/1‑149 (MQ=255)

                                                                                                                                     tGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCtctc           <  1:207397/149‑1 (MQ=255)

                                                                                                                                      gATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCTCTcc          >  5:181407/1‑149 (MQ=255)

                                                                                                                                       aTCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCTCTCCt         >  8:210871/1‑149 (MQ=255)

                                                                                                                                        tCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCTCTCCTg        <  3:198991/149‑1 (MQ=255)

                                                                                                                                           gCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCTCTCCTGTAt     >  3:174242/1‑149 (MQ=255)

                                                                                                                                            cGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTGTTACCGCGTTTTTGTTACCAGGTGAAGGTACTGATGGGCTTGCAGCAAGACGGTAACGCTTTCATGTATc    >  7:111003/1‑149 (MQ=255)

                                                                                                                                             gTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCG‑TTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCTCTCCTGTATCCg  >  6:152737/1‑149 (MQ=255)

                                                                                                                                              ttGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCTCTCCTGTATCCg  >  3:4184/1‑149 (MQ=255)

CCAGGTCTCGGTCTTGCCACTGATGGCCCGTGCCTGGCGGGTGGATCGGACGTTCATGCCGTTGCGGTTCTGCACCTGGGGTCGGGATTTCAGTCTCCCGCCTTCTCGCCAGCTGTAGCGAATGGCGCCCTGGTGATCGGCGTTGCCGATCGGGTCCAGTACGTTGTGCTGCTCCACGTCGTCCGGGCCGATGGTGCAGCATTGCTCGCCCCAG‑TCGTCGTACCGCGTTTTTGTTACCAGGTGCAGGTACTGCTGGGCTTGCAGCCAGTCGGTAACGCTCTCCTGTATCCG  >  NC_002947/2111892‑2112182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`