Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,121,033 C→T 8.3% A728A (GCC→GCT)  PP_1885 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,121,0330CT 8.3% 134.2 / 2.8 48A728A (GCC→GCT) PP_1885hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/24);  new base T (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGCCCGACAGGCCTGCGTGTGGCCA  >  NC_002947/2120885‑2121181
                                                                                                                                                    |                                                                                                                                                         
aCGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGcc                                                                                                                                                           >  2:117444/1‑149 (MQ=255)
  gTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCTGa                                                                                                                                                         >  6:133378/1‑149 (MQ=255)
       gCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATgcgc                                                                                                                                                    <  5:319436/149‑1 (MQ=255)
                cGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCgcgc                                                                                                                                           >  7:104000/1‑149 (MQ=255)
                 gATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCt                                                                                                                                          <  6:360231/149‑1 (MQ=255)
                   tGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGa                                                                                                                                        >  5:218274/1‑149 (MQ=255)
                       gACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCTGATGCGCGTTTCGCGCTGATTAc                                                                                                                                    <  5:133378/149‑1 (MQ=255)
                        aCCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTAcc                                                                                                                                   <  6:306525/149‑1 (MQ=255)
                          cTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCTGATGCGCGTTTCGCGCTGATTACCGc                                                                                                                                 >  4:344913/1‑149 (MQ=255)
                          cTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGc                                                                                                                                 >  3:229232/1‑149 (MQ=255)
                           tGCAGTTGACAGGTATGTTCGATGGGGTAGACCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGc                                                                                                                                 >  2:370286/1‑148 (MQ=255)
                             cAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCa                                                                                                                               >  8:164363/1‑148 (MQ=255)
                                ttGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATg                                                                                                                           >  8:321800/1‑149 (MQ=255)
                                              cGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGt                                                                                                           <  6:6607/148‑1 (MQ=255)
                                               gATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGACGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGt                                                                                                           >  7:302593/1‑147 (MQ=255)
                                               gATGGGGTAGACCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGtt                                                                                                          <  1:370286/148‑1 (MQ=255)
                                                aTGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCt                                                                                                        >  5:19458/1‑149 (MQ=255)
                                                    ggTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCt                                                                                                  <  4:229232/149‑1 (MQ=255)
                                                         gccagccaGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATc                                                                                             <  3:269188/149‑1 (MQ=255)
                                                                agACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAg                                                                                      <  7:321800/149‑1 (MQ=255)
                                                                     cccAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCAc                                                                                 <  7:164363/149‑1 (MQ=255)
                                                                               aTTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGc                                                                       <  6:217670/149‑1 (MQ=255)
                                                                                  gCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGc                                                                    >  6:319902/1‑149 (MQ=255)
                                                                                   ccAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGtt                                                                                                          <  1:294920/112‑1 (MQ=255)
                                                                                   ccAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGtt                                                                                                          >  2:294920/1‑112 (MQ=255)
                                                                                          cAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCtt                                                            >  8:217896/1‑149 (MQ=255)
                                                                                             ggACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTcc                                                         >  6:249683/1‑149 (MQ=255)
                                                                                                     gagaTCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACtt                                                 <  8:104000/149‑1 (MQ=255)
                                                                                                      agaTCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTc                                                <  5:319902/149‑1 (MQ=255)
                                                                                                        aTCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGa                                              <  1:255520/149‑1 (MQ=255)
                                                                                                           gATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACg                                            <  5:249683/148‑1 (MQ=255)
                                                                                                                ggCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCAc                                      >  4:275017/1‑149 (MQ=255)
                                                                                                                        gCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCt                              <  6:218274/149‑1 (MQ=255)
                                                                                                                               ttCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGGCCTCTATTCCAGGTTCTACATCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGc                            <  1:117444/149‑1 (MQ=255)
                                                                                                                               ttCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAg                         <  3:275017/147‑1 (MQ=255)
                                                                                                                               ttCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGcc                       <  8:229380/149‑1 (MQ=255)
                                                                                                                                 ccccTGCATCTGCCGACGCTGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGccc                      <  3:344913/148‑1 (MQ=255)
                                                                                                                                 ccccTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCCGCCGCTGccc                                                               <  7:190580/107‑1 (MQ=255)
                                                                                                                                 ccccTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCCGCCGCTGccc                                                               >  8:190580/1‑107 (MQ=255)
                                                                                                                                  cccTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGCCCGa                    >  2:345626/1‑149 (MQ=255)
                                                                                                                                    cTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGCCCGa                    >  2:27954/1‑147 (MQ=255)
                                                                                                                                    cTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGCCCGAc                   >  6:313137/1‑148 (MQ=255)
                                                                                                                                    cTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGCCCGAc                   <  5:313137/148‑1 (MQ=255)
                                                                                                                                     tGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCAtt                                                                           <  3:254103/91‑1 (MQ=255)
                                                                                                                                     tGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCAtt                                                                           >  4:254103/1‑91 (MQ=255)
                                                                                                                                          cTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCt                                                                               >  6:105444/1‑82 (MQ=255)
                                                                                                                                          cTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCt                                                                               <  5:105444/82‑1 (MQ=255)
                                                                                                                                                aCGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGCCCGACAGGCCTGCGtgtg      >  6:16311/1‑149 (MQ=255)
                                                                                                                                                aCGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGATCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGCCCGACAGGCCTGCGtgtg      <  6:19458/149‑1 (MQ=255)
                                                                                                                                                    cGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGCCCGACAGGCCTGCGTGTGGCca  <  1:27954/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                         
ACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATTGCAGGGACTGGCGAGATCGATGAGGCCACTGGCCGCTATTCCCCTGCATCTGCCGACGCCGATGCGCGTTTCGCGCTGATTACCGCCAAATGGG‑‑‑CGATTCCAGGTTCT‑‑GTCTTCATCCTGGAAGGTCACCTCATTCTGCCGCTGCCCCTTTCCTTGCACTTCGACGCTGCACGGCGCGCTGCCAGCCCGACAGGCCTGCGTGTGGCCA  >  NC_002947/2120885‑2121181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑