Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,246,241 C→T 8.0% intergenic (+30/+61) ibpA → / ← PP_1983 small heat shock protein IbpA/PAS/PAC/GAF sensor‑containing diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,246,2410CT 8.0% 176.9 / 4.7 62intergenic (+30/+61)ibpA/PP_1983small heat shock protein IbpA/PAS/PAC/GAF sensor‑containing diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (24/33);  new base T (3/2);  total (27/35)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

AGTGAAGGCCGCCGGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTGGTA  >  NC_002947/2246094‑2246382
                                                                                                                                                   |                                                                                                                                             
aGTGAAGGCCGCCGGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCa                                                                                                                                              <  7:270861/149‑1 (MQ=255)
   gAAGGCCGCCGGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAATAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCaga                                                                                                                                            <  8:318584/148‑1 (MQ=255)
     aGGCCGCCGGCCTGGCCAATTGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGTAGCCAAGGCCAAGCTCATTCCGATCAATGGCGAAAACCCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGt                                                                                                                                         <  6:212059/149‑1 (MQ=255)
        ccgccgGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGcc                                                                                                                                      >  4:349938/1‑149 (MQ=255)
         cgccgGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGccc                                                                                                                                     <  2:56882/149‑1 (MQ=255)
             ggCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCtttt                                                                                                                                 <  1:351839/149‑1 (MQ=255)
              gCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCttttt                                                                                                                                <  6:254485/149‑1 (MQ=255)
              gCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCttttt                                                                                                                                <  6:248872/149‑1 (MQ=255)
                cTGGCCACTGGCCTGCTGAGCATCGACCTGCTGCTCTTCGTGCCTGAAGATGCCAAGGCCTTGCGCATTCCGTTGAATGGCTAAAAACCAGCGCTGATCTTATCGAGCTTCAACTTAAAATACTGCACCTACAGAGGTGCCCTTTTTCg                                                                                                                              <  4:139255/149‑1 (MQ=255)
                 tGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGt                                                                                                                             >  3:120173/1‑149 (MQ=255)
                  ggCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCTGGAGGTGTCCtt                                                                                                                                   >  6:188165/1‑142 (MQ=255)
                  ggCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCTGGAGGTGTCCtt                                                                                                                                   <  5:188165/142‑1 (MQ=255)
                   gCCAATGGCCTGCTCATCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAAGCAGCGCTTAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTg                                                                                                                            <  5:21729/148‑1 (MQ=255)
                          gCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCAc                                                                                                                    <  4:75262/149‑1 (MQ=255)
                                tCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTc                                                                                                              >  7:62710/1‑149 (MQ=255)
                                    cATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGt                                                                                                                             >  2:286025/1‑130 (MQ=255)
                                    cATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGt                                                                                                                             <  1:286025/130‑1 (MQ=255)
                                     aTCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTg                                                                                                                            >  7:261050/1‑130 (MQ=255)
                                     aTCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCcgcg                                                                                                         >  5:174067/1‑149 (MQ=255)
                                     aTCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCcgc                                                                                                          <  7:274755/148‑1 (MQ=255)
                                     aTCGACCTGCTGAGCATCTTGCCTGAAGAAGCCAAGTCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGTGCACCTCCAGAGGTGCCCTTTTTCTTg                                                                                                                            <  8:261050/130‑1 (MQ=255)
                                      tCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGt                                                                                                        <  5:61535/149‑1 (MQ=255)
                                        gACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAg                                                                                                      <  3:349938/149‑1 (MQ=255)
                                              ctgcGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTgcgc                                                                                                >  3:263101/1‑149 (MQ=255)
                                                  gcATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGcc                                                                                            <  5:151513/149‑1 (MQ=255)
                                                    aTCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGt                                                                                          <  4:263101/149‑1 (MQ=255)
                                                           cTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGa                                                                                   >  2:250957/1‑149 (MQ=255)
                                                           cTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGa                                                                                   <  5:302686/149‑1 (MQ=255)
                                                                    ccaaggccaagCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATc                                                                          >  1:355548/1‑149 (MQ=255)
                                                                     caaggccaagCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGt                                                                                                    >  7:253747/1‑122 (MQ=255)
                                                                     caaggccaagCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGt                                                                                                    <  8:253747/122‑1 (MQ=255)
                                                                     caaggccaagCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCt                                                                         >  2:355983/1‑149 (MQ=255)
                                                                           caagCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGgc                                                                   <  6:174067/149‑1 (MQ=255)
                                                                                 cATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTc                                                              >  5:177578/1‑148 (MQ=255)
                                                                                   ttCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAg                                                           >  8:313058/1‑149 (MQ=255)
                                                                                     ccGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGc                                                          <  4:47014/148‑1 (MQ=255)
                                                                                            aTGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAg                                                                               >  1:47541/1‑120 (MQ=255)
                                                                                            aTGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAg                                                                               <  2:47541/120‑1 (MQ=255)
                                                                                               gCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGcc                                                >  4:189696/1‑148 (MQ=255)
                                                                                                cGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGa                                              <  6:140144/149‑1 (MQ=255)
                                                                                                   aaaaCCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCCCCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAAACTGCCGGCGCCTCAAGCAACATCTGCCGAAAc                                           >  5:166999/1‑149 (MQ=255)
                                                                                                     aaCCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTc                                         <  4:86560/149‑1 (MQ=255)
                                                                                                       ccAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcg                                       <  4:120173/149‑1 (MQ=255)
                                                                                                         tgcgcTGAACTGATCGAGCTGCAAAGATAAAAATGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGAAGTCCGGCGTATGTGCGCGGCCGTCATGCGATCAGTAATCTGCCTGCGCCTCTAGCAACATCTGCCGAAACTAcggt                                     <  6:166999/148‑1 (MQ=255)
                                                                                                                aaCTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcggtaacggta                              <  7:313058/149‑1 (MQ=255)
                                                                                                                             gCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCtgtg                 >  2:17480/1‑149 (MQ=255)
                                                                                                                              cAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcgg                                      >  2:356949/1‑127 (MQ=255)
                                                                                                                              cAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcgg                                      <  1:356949/127‑1 (MQ=255)
                                                                                                                              cAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGc                <  3:13342/149‑1 (MQ=255)
                                                                                                                                gcacgaaaaaGGGCACCTCTGGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTc                                         >  7:166803/6‑122 (MQ=255)
                                                                                                                                gcacgaaaaaGGGCACCTCTGGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTc                                         <  8:166803/117‑1 (MQ=255)
                                                                                                                                aCGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCtgtg                 >  5:226065/1‑146 (MQ=255)
                                                                                                                                  gAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcggtaacg                                 >  6:351407/1‑128 (MQ=255)
                                                                                                                                  gAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCcggtaacg                                 <  5:351407/128‑1 (MQ=255)
                                                                                                                                    gaaaaaGGGCACCTCTGGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATaa          >  2:81045/2‑149 (MQ=255)
                                                                                                                                      aaaaGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAAcc        <  8:62710/149‑1 (MQ=255)
                                                                                                                                         aGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTg     <  1:250957/149‑1 (MQ=255)
                                                                                                                                          gggCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATGAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTgg    >  6:322113/1‑149 (MQ=255)
                                                                                                                                             cACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTGGTa  >  3:248474/1‑148 (MQ=255)
                                                                                                                                                cTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCtg                   >  4:73513/1‑128 (MQ=255)
                                                                                                                                                cTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCtg                   <  3:73513/128‑1 (MQ=255)
                                                                                                                                                cTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTGGTa  >  8:232785/1‑145 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
AGTGAAGGCCGCCGGCCTGGCCAATGGCCTGCTCAGCATCGACCTGCTGCGCATCGTGCCTGAAGAAGCCAAGGCCAAGCGCATTCCGATCAATGGCGAAAAACCAGCGCTGAACTGATCGAGCTGCAACGAAAAAAAGGGCACCTCCAGAGGTGCCCTTTTTCGTGCTTCTCACGCAGTCCCGCGTAGGTGCGCGGCCGTCATGCGATCAGTAATCTGCCGGCGCCTCCAGCAACATCTGCCGAAACTCCGGTAACGGTAATGGCCGGCTGTGCAGATAACCCTGGTA  >  NC_002947/2246094‑2246382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑