Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,266,896	G→A	8.5%	intergenic (+17/‑180)	dedD → / → cvpA	cell division protein/colicin V production membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,266,896	0	G	A	8.5%	164.8 / 3.6	58	intergenic (+17/‑180)	dedD/cvpA	cell division protein/colicin V production membrane protein
Reads supporting (aligned to +/- strand):  ref base G (23/30);  new base A (3/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGCTTACTCCCGGCCCTCTGCTCTGGTAAAA  >  NC_002947/2266757‑2267044                                                                                                                                             |                                                                                                                                                     aTGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCAt                                                                                                                                             >  5:372661/1‑149 (MQ=255)  tGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCCTAGCCGccccgg                                                                                                                                            <  4:70329/149‑4 (MQ=255)    aaCCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTcg                                                                                                                                          <  7:298681/149‑1 (MQ=255)      ccGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTcgc                                                                                                                                         >  6:138786/1‑148 (MQ=255)      ccGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTcgc                                                                                                                                         <  5:138786/148‑1 (MQ=255)           tttACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGa                                                                                                                                   <  3:190238/149‑1 (MQ=255)             tACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAg                                                                                                                                  <  3:141365/148‑1 (MQ=255)              aCGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCgg                                                                                                                                       <  3:20354/142‑1 (MQ=255)              aCGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCgg                                                                                                                                       >  4:20354/1‑142 (MQ=255)                  ggggCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGATGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCcgc                                                                                                                             >  1:57419/1‑149 (MQ=255)                   gggCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCgcgc                                                                                                                           >  5:192424/1‑149 (MQ=255)                     gCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCCTAGCCGccccgg                                                                                                                                            >  8:317304/1‑127 (MQ=255)                     gCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCCTAGCCGccccgg                                                                                                                                            <  7:317304/130‑4 (MQ=255)                     gCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGAGTACCCGGGGCGGCTAGGCCGCCCATTCGCGGGTCAGCCCGCGCCt                                                                                                                         >  4:45777/1‑149 (MQ=255)                      ccGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGTGGCGGATAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTg                                                                                                                        <  8:10798/149‑1 (MQ=255)                      ccGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTg                                                                                                                        <  2:57419/149‑1 (MQ=255)                         ttGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAg                                                                                                                     <  4:72900/149‑1 (MQ=255)                          tGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGt                                                                                                                                     <  6:359587/132‑1 (MQ=255)                                aGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACttt                                                                                                              <  5:12940/149‑1 (MQ=255)                                    cgcCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCg                                                                                                          <  5:277671/149‑1 (MQ=255)                                     gccgaggccgagCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGAGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGc                                                                                                         >  7:248179/1‑149 (MQ=255)                                      ccgaggccgagCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCt                                                                                                        <  6:127116/149‑1 (MQ=255)                                       cgaggccgagCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCCCTTCCAGACAGAGCGCAGCTGATTCCCCGGGGCGGCTAGGACGCCCATTCGCGGGTGAGCCCGCGCCTGCACGTACTTTGCCGCTg                                                                                                       >  1:139445/1‑149 (MQ=255)                                             cgagCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGc                                                                                                 >  4:352265/1‑149 (MQ=255)                                                   cg‑tgcgtgACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTAccc                                                                                           >  4:194863/1‑149 (MQ=255)                                                         gtgACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCGAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGCGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGatc                                                                                      >  8:152218/1‑147 (MQ=255)                                                         gtgACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAAc                                                                                      <  6:192424/149‑1 (MQ=255)                                                         gggACGCCAGCACCCGGCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCCTTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAAc                                                                                      <  6:391380/147‑1 (MQ=255)                                                           gACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCt                                                                                    <  5:300921/149‑1 (MQ=255)                                                                 aTCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCggg                                                                              <  6:372661/149‑1 (MQ=255)                                                                     aCCGTCATTACAGCGTCATGGGCTTCGTGGTGCTCTTCCAGCCTGTGAGCAGCTGATTCCCCGGGGCGGGTAGGCGGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGGCGCTGCAAGGCTTACGCGGAACCTGTGGGGGCgg                                                                          <  2:139445/149‑1 (MQ=255)                                                                     aCCGTCAGAACAGCCTCAAGGGCTTCGGGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCgg                                                                          <  5:190297/149‑1 (MQ=255)                                                                          cAGAACAGCCTCACGGGCTTCGTGGTGCGCGTCCAGCCTGCGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTtg                                                                     <  8:260266/149‑1 (MQ=255)                                                                             aaCAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTtgcct                                                                  >  2:178265/1‑149 (MQ=255)                                                                                  ccTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCaa                                                             >  2:185537/1‑149 (MQ=255)                                                                                    tCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAg                                                                                                                     <  8:241349/91‑1 (MQ=255)                                                                                    tCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAg                                                                                                                     >  7:241349/1‑91 (MQ=255)                                                                                      aaGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTtgcctgcc                                                               >  8:222025/1‑143 (MQ=255)                                                                                      aaGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTtgcctgcc                                                               <  7:222025/143‑1 (MQ=255)                                                                                      aaGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGc                                                         >  7:233002/1‑149 (MQ=255)                                                                                        gggCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGATAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGccc                                                       <  4:347117/149‑1 (MQ=255)                                                                                              cGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGTCTAGGCCTCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAacac                                                  <  1:158308/148‑1 (MQ=255)                                                                                                  gtgCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAAt                                              <  6:306553/148‑1 (MQ=255)                                                                                                    gcgcTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGACTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATc                                             >  2:30789/1‑147 (MQ=255)                                                                                                       cTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCt                                        <  8:18028/149‑1 (MQ=255)                                                                                                       cTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCt                                        >  3:70987/1‑149 (MQ=255)                                                                                                              ccTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTc                                 >  7:191434/1‑149 (MQ=255)                                                                                                                  aGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGAGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGc                              <  7:359814/148‑1 (MQ=255)                                                                                                                          tGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGCTTACTCCCg                     >  4:114993/1‑149 (MQ=255)                                                                                                                            aTTCCCCGGGGCGTCTAGGCCGCCCAGTCGCGTGTGAGCCAGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTTACATTCCGCTTACTCCCgg                    <  3:45777/148‑1 (MQ=255)                                                                                                                            aTTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGccc                                                       <  3:371855/113‑1 (MQ=255)                                                                                                                            aTTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGccc                                                       >  4:371855/1‑113 (MQ=255)                                                                                                                            aTTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGCTTACTCCCGGc                   >  8:211023/1‑149 (MQ=255)                                                                                                                             ttCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGCTTACTCCCGGcc                  >  8:266055/1‑149 (MQ=255)                                                                                                                              gcgaatgggCGGCCTAGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGCTTACTCCCGGccc                 >  4:43822/7‑149 (MQ=255)                                                                                                                                cccGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGCTTACTCCCGGCCctc               <  8:233002/149‑1 (MQ=255)                                                                                                                                cccGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGCTTACTCCCGGCCctc               <  8:248179/149‑1 (MQ=255)                                                                                                                                  atgggCGGCCTAGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGTCCCAACACCAATACAGGTGACATTCCGCTTAGTCGCGGCCctctg             >  3:305325/3‑149 (MQ=255)                                                                                                                                             ggCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGCTTACTCCCGGCCCTCTGCTCTGGTaaaa  <  1:185537/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                     ATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCG‑TGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTCCAGCCTGAGCGCAGCTGATTCCCCGGGGCGGCTAGGCCGCCCATTCGCGGGTGAGCCCGCGCCTGCAGGTACTTTGCCGCTGCAAGGCTTACCCGGAACCTGTCGGGGCGGGTTTGCCTGCCAATGGCCCAACACCAATCCAGCTGACATTCCGCTTACTCCCGGCCCTCTGCTCTGGTAAAA  >  NC_002947/2266757‑2267044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑