Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,281,030 T→A 7.7% intergenic (+88/‑149) fadH → / → PP_2009 2,4‑dienoyl‑CoA reductase/1‑aminocyclopropane‑1‑carboxylate deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,281,0300TA 7.7% 156.5 / 2.8 52intergenic (+88/‑149)fadH/PP_20092,4‑dienoyl‑CoA reductase/1‑aminocyclopropane‑1‑carboxylate deaminase
Reads supporting (aligned to +/- strand):  ref base T (26/22);  new base A (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CGAGCTGGATGCCAAGCGGGCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCGGGATTACCCTGTAAACTGCGAT  >  NC_002947/2280885‑2281176
                                                                                                                                                 |                                                                                                                                                    
cGAGCTGGATGCCAAGCGGGCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGc                                                                                                                                                  <  8:102289/149‑1 (MQ=255)
      ggATGCCAAGCGGGCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGgtgt                                                                                                                                            >  7:177326/1‑149 (MQ=255)
         tGCCAAGCGGGCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACAGGCAT‑TGCCGGTGTTcg                                                                                                                                         >  8:337949/1‑149 (MQ=255)
             aaGCGGGCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCg                                                                                                                                                >  4:66106/1‑138 (MQ=255)
             aaGCGGGCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCg                                                                                                                                                <  3:66106/138‑1 (MQ=255)
             aaGCGGGCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAA‑TGCCGgtgc                                                                                                                                            <  3:155459/142‑2 (MQ=255)
             aaGCGGGCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAA‑TGCCGgtgc                                                                                                                                            >  4:155459/1‑141 (MQ=255)
                   gCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAAcc                                                                                                                               >  5:230269/1‑149 (MQ=255)
                   gCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAAcc                                                                                                                               >  7:8310/1‑149 (MQ=255)
                     gATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCg                                                                                                                             >  5:194730/1‑149 (MQ=255)
                       tCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGgt                                                                                                                                              >  3:36761/1‑130 (MQ=255)
                       tCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGgt                                                                                                                                              <  4:36761/130‑1 (MQ=255)
                         aaCCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTc                                                                                                                          <  6:196292/148‑1 (MQ=255)
                               ggCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTacgac                                                                                                                   <  7:337949/149‑1 (MQ=255)
                                  tCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAA‑TGCCGgtgcca                                                                                                                                          >  7:159808/1‑120 (MQ=255)
                                  tCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAA‑TGCCGgtgcca                                                                                                                                          <  8:159808/123‑4 (MQ=255)
                                                  aGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCAt                                                                                                  <  6:230269/147‑1 (MQ=255)
                                                       tGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGcc                                                                                           >  8:250982/1‑149 (MQ=255)
                                                         aGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCt                                                                                          <  6:194730/148‑1 (MQ=255)
                                                          ggTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCtgt                                                                                        <  7:278380/149‑1 (MQ=255)
                                                               tCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTc                                                                                     >  5:230026/1‑147 (MQ=255)
                                                               tCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGa                                                                                   <  8:8310/149‑1 (MQ=255)
                                                                cGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAg                                                                                  <  8:177326/149‑1 (MQ=255)
                                                                         gTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTgg                                                                         >  3:265545/1‑149 (MQ=255)
                                                                          tGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGc                                                                        <  6:230026/149‑1 (MQ=255)
                                                                               ccGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACtgt                                                                   >  6:224083/1‑149 (MQ=255)
                                                                               ccGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACtgt                                                                   >  5:329259/1‑149 (MQ=255)
                                                                                cGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACtgt                                                                   >  4:24321/1‑148 (MQ=255)
                                                                                 gACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGa                                                                 <  1:333210/149‑1 (MQ=255)
                                                                                      cTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCAccc                                                                            >  5:126622/1‑133 (MQ=255)
                                                                                      cTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCAccc                                                                            <  6:126622/133‑1 (MQ=255)
                                                                                      cTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑t                                                           >  2:68043/1‑149 (MQ=255)
                                                                                                      gggCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCa                                           >  3:329610/1‑149 (MQ=255)
                                                                                                           tGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGAt                                      <  4:265545/149‑1 (MQ=255)
                                                                                                              ccGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGAGGCCTGTGTCGAGCCAccc                                                                            <  7:4496/109‑1 (MQ=255)
                                                                                                              ccGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGAAGCCTGTGTCGAGCCAccc                                                                            >  8:4496/1‑109 (MQ=255)
                                                                                                               cgcgAAACAGGTGACGCGGTGGATGGCACCGGCATATGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑Tcggcg                                                      >  1:248982/1‑130 (MQ=255)
                                                                                                               cgcgAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑Tcggcg                                                      <  2:248982/129‑1 (MQ=255)
                                                                                                                    aaCAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAgcgc                             <  5:312246/149‑1 (MQ=255)
                                                                                                                    aaCAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAgcgc                             <  3:24321/149‑1 (MQ=255)
                                                                                                                        ggTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGc                         >  6:163130/1‑149 (MQ=255)
                                                                                                                        ggTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGc                         >  8:155648/1‑149 (MQ=255)
                                                                                                                          tGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCgg                       >  3:50822/1‑149 (MQ=255)
                                                                                                                            aCGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCCTTCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCgg                       <  1:68043/148‑1 (MQ=255)
                                                                                                                            aCGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCGGGa                     <  4:50822/149‑1 (MQ=255)
                                                                                                                                ggTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCGGGATTAc                 >  7:357761/1‑149 (MQ=255)
                                                                                                                                 gTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCGGGATTAcc                <  8:450/149‑1 (MQ=255)
                                                                                                                                         cACCGGCAT‑TGCTGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACaa                                 >  4:50025/1‑124 (MQ=255)
                                                                                                                                         cACCGGCAT‑TGCTGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACaa                                 <  3:50025/124‑1 (MQ=255)
                                                                                                                                          aCCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCGGGATTACCCTGTAAACt       >  6:311005/1‑149 (MQ=255)
                                                                                                                                          aCCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCGGGATTACCCTGTAAACt       >  1:205239/1‑149 (MQ=255)
                                                                                                                                               cAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCGGGATTACCCTGTAAACTGCGAt  >  4:57593/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGAGCTGGATGCCAAGCGGGCGATCAACCAAGGCTCGCGGTTGGCGGCTGAGCTCTGAGGTTGTCGGGGCTGCGTGAGCCCGACACCTGCACGCGTGGGAGCGGGCATGCCCGCGAAACAGGTGACGCGGTGGATGGCACCGGCAT‑TGCCGGTGTTCGCGGGTGAACCCGCTCCTACGACGGGCTGTGCCGGGGCATCGATGCCTGTGTCGAGCCACCCTGGCACTGTGATGCC‑TCGGCGGGGGCGGGTCATCGATTACAAGCGCTTGCGGGATTACCCTGTAAACTGCGAT  >  NC_002947/2280885‑2281176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑