Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,291,992 G→A 7.0% V129V (GTG→GTA) ‡ PP_2019 → RND family multidrug transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,291,9920GA 7.0% 166.1 / 2.4 57V129V (GTG→GTA) ‡PP_2019RND family multidrug transporter
Reads supporting (aligned to +/- strand):  ref base G (25/28);  new base A (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

GCAACACCGTGCGCATCTCGGTGGAGGCGGTGAACGGTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTGGTAGGCAACAACTTCAA  >  NC_002947/2291844‑2292138
                                                                                                                                                    |                                                                                                                                                  
gCAACACCGTGCGCATCTCGGTGGAGGCGGTGAACGGTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTg                                                                                                                                                    >  1:12916/1‑149 (MQ=255)
       cGTGCGCATCTCGGTGGAGGCGGTGAACGGTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGa                                                                                                                                             <  2:65964/149‑1 (MQ=255)
        gTGCGCATCTCGGTGGAGGCGGTGAACGGTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCACGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAg                                                                                                                                            >  2:368646/1‑149 (MQ=255)
           cgcATCTCGGTGGAGGCGGTGAACGGTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCTCTGTGGAGCTCCAGCGCACGCTGGGGctc                                                                                                                                         <  8:156143/149‑3 (MQ=255)
                        aGGCGGTGAACGGTGACATCTTCGACAAGGTCTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAagag                                                                                                                            <  6:18773/149‑1 (MQ=255)
                         ggCGGTGAACGGTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAgg                                                                                                                           <  5:376831/149‑1 (MQ=255)
                           cGGTGAACGGTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGt                                                                                                                         <  7:290515/149‑1 (MQ=255)
                             gTGAACGGTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGttt                                                                                                                       >  3:248979/1‑149 (MQ=255)
                             gTGAACGGTGACATCTTCGACAAGGACTACATGGAAACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGttt                                                                                                                       >  1:247400/1‑149 (MQ=255)
                                    gTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGt                                                                                                                >  7:256103/1‑149 (MQ=255)
                                    gTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGt                                                                                                                <  1:368646/149‑1 (MQ=255)
                                        cATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCg                                                                                                            <  8:256103/149‑1 (MQ=255)
                                             tCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGt                                                                                                        >  6:182792/1‑148 (MQ=255)
                                               gACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCAt                                                                                                     >  7:79089/1‑149 (MQ=255)
                                                aCAAGGACTACATGGATACCCTGCGTCATATCCATGACGATGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCgg                                                                                                                 <  4:242087/136‑1 (MQ=255)
                                                aCAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCgg                                                                                                                 >  3:242087/1‑136 (MQ=255)
                                                aCAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATc                                                                                                    >  1:374035/1‑149 (MQ=255)
                                                     gACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGa                                                                                                           >  6:342042/1‑137 (MQ=255)
                                                     gACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGa                                                                                                           <  5:342042/137‑1 (MQ=255)
                                                          cATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACAcc                                                                                           >  2:357102/1‑148 (MQ=255)
                                                          cATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACAcc                                                                                           <  4:248979/148‑1 (MQ=255)
                                                              gagaCCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAgg                                                                                                         >  3:188411/1‑130 (MQ=255)
                                                              gagaCCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAgg                                                                                                         <  4:188411/130‑1 (MQ=255)
                                                                    cTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTc                                                                                <  4:345535/149‑1 (MQ=255)
                                                                         tCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAgg                                                                                                         <  7:251397/119‑1 (MQ=255)
                                                                         tCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAgg                                                                                                         >  8:251397/1‑119 (MQ=255)
                                                                           acaTCCATGACGAGTTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTTGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACAACTACAACGGGTCGCAGGAc                                                                         <  5:334258/147‑1 (MQ=255)
                                                                            gATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGa                                                                          >  7:24658/1‑147 (MQ=255)
                                                                                      gAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGt                                                                                                                <  4:175285/99‑1 (MQ=255)
                                                                                      gAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGt                                                                                                                >  3:175285/1‑99 (MQ=255)
                                                                                      gAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTaa                                                                                               <  1:251723/116‑1 (MQ=255)
                                                                                      gAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTaa                                                                                               >  2:251723/1‑116 (MQ=255)
                                                                                       aGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATAcc                                                             >  6:262873/1‑149 (MQ=255)
                                                                                        ggTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATAccc                                                            <  2:12916/149‑1 (MQ=255)
                                                                                            ttCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATcc                                                                                                   <  8:158702/106‑1 (MQ=255)
                                                                                            ttCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCACCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATcc                                                                                                   >  7:158702/1‑106 (MQ=255)
                                                                                              cTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGAGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTACCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTgcgc                                                       >  2:26432/1‑148 (MQ=255)
                                                                                                 cATCCCCGGTGGCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGcgcg                                                      <  7:264324/146‑1 (MQ=255)
                                                                                                    ccccGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACg                                                >  4:74652/1‑149 (MQ=255)
                                                                                                      ccGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTg                                              >  1:16293/1‑149 (MQ=255)
                                                                                                         gtgtCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTc                                           <  8:79089/149‑1 (MQ=255)
                                                                                                              gACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGt                                       >  2:269245/1‑148 (MQ=255)
                                                                                                              gACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGt                                       >  8:276427/1‑148 (MQ=255)
                                                                                                               aCCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCa                                     <  3:21702/149‑1 (MQ=255)
                                                                                                                  gggccgggcTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGc                                  <  1:357102/149‑1 (MQ=255)
                                                                                                                   ggccgggcTGAAGTCACTGTGGAGCCCCAGCGTGCGATGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGc                                  >  3:70323/1‑148 (MQ=255)
                                                                                                                     ccgggcTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAg                               <  8:24658/149‑1 (MQ=255)
                                                                                                                                tCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCt                    <  7:276427/149‑1 (MQ=255)
                                                                                                                                 cACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTg                   >  7:278162/1‑149 (MQ=255)
                                                                                                                                    tgtgGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTgg                  >  6:16731/1‑147 (MQ=255)
                                                                                                                                      tgGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTGGTa                <  2:16293/147‑1 (MQ=255)
                                                                                                                                      tgGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTGGTa                <  3:74652/147‑1 (MQ=255)
                                                                                                                                      tgGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTGGTAgg              <  1:26432/149‑1 (MQ=255)
                                                                                                                                      tgGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTGGTAg               <  8:278162/148‑1 (MQ=255)
                                                                                                                                       ctcgctccAGCGCACGCTGGGGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCAACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTg                   <  7:298417/137‑1 (MQ=255)
                                                                                                                                       ctcgctccAGCGCACGCTGGGGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCAACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTg                   >  8:298417/7‑143 (MQ=255)
                                                                                                                                        tcgctccAGCGCACGCTGGGGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTGGTAGGc             >  8:108358/6‑148 (MQ=255)
                                                                                                                                                  gTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGTTGTGGCGCCTGGTAGGCAACAACTTCaa  <  4:70323/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCAACACCGTGCGCATCTCGGTGGAGGCGGTGAACGGTGACATCTTCGACAAGGACTACATGGAGACCCTGCGTCAGATCCATGACGAGGTGTTCTACATCCCCGGTGTCGACCGGGCCGGGCTGAAGTCACTGTGGAGCCCCAGCGTGCGCTGGAGCGAGGTCACCGAAGAGGGTTTTTCCGGTGGCGAGGTCATCCCTAACACCTACAACGGGTCGCAGGACAGCCTCGATACCCTGCGCGACAACGTGCTCAAGTCAGGCCAGGTGGGGCGCCTGGTAGGCAACAACTTCAA  >  NC_002947/2291844‑2292138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑