Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,311,049 T→C 7.1% V267A (GTA→GCA) ‡ PP_2033 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,311,0490TC 7.1% 203.1 / 4.6 70V267A (GTA→GCA) ‡PP_2033hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (35/30);  new base C (3/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAG  >  NC_002947/2310909‑2311185
                                                                                                                                            |                                                                                                                                        
gcggcgGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCAcgc                                                                                                                                  <  3:43375/149‑1 (MQ=255)
        aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGTTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAcgcac                                                                                                                                <  8:265173/143‑3 (MQ=255)
        aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGGTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAcgcac                                                                                                                                >  7:265173/1‑141 (MQ=255)
        aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAg                                                                                                                          >  8:217235/1‑149 (MQ=255)
           aCTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCa                                                                                                                        <  3:4243/148‑1 (MQ=255)
             ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTg                                                                                                                     >  2:170303/1‑149 (MQ=255)
             ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTg                                                                                                                     >  5:43296/1‑149 (MQ=255)
             ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTg                                                                                                                     >  6:156990/1‑149 (MQ=255)
             ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAcgcactgg                                                                                                                             >  5:188611/1‑136 (MQ=255)
             ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAcgcactgg                                                                                                                             <  6:188611/141‑6 (MQ=255)
               cccGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCAcgc                                                                                                                                  <  4:21346/134‑1 (MQ=255)
               cccGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCAcgc                                                                                                                                  >  3:21346/1‑134 (MQ=255)
                 cGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGTGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCcgc                                                                                                                 <  4:40757/149‑1 (MQ=255)
                  gCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCcgcg                                                                                                                >  8:155045/1‑149 (MQ=255)
                       tGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACAt                                                                                                           >  7:115001/1‑149 (MQ=255)
                         cgcAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCAcgc                                                                                                                                  >  3:37518/1‑124 (MQ=255)
                         cgcAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCAcgc                                                                                                                                  <  4:37518/124‑1 (MQ=255)
                             gTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAg                                                                                                     <  6:350517/149‑1 (MQ=255)
                                gCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                   <  7:155045/148‑1 (MQ=255)
                                 ccACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCg                                                                                                                            >  2:11181/1‑122 (MQ=255)
                                 ccACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCg                                                                                                                            <  1:11181/122‑1 (MQ=255)
                                 ccACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGa                                                                                                 <  6:43296/149‑1 (MQ=255)
                                           gTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCt                                                                                       >  6:339036/1‑149 (MQ=255)
                                           gTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCt                                                                                       <  3:9621/149‑1 (MQ=255)
                                                           aCTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                          <  7:299703/146‑1 (MQ=255)
                                                           aCTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                          >  8:299703/1‑146 (MQ=255)
                                                             tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                   <  2:100051/119‑1 (MQ=255)
                                                             tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                   >  1:100051/1‑119 (MQ=255)
                                                             tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                     >  7:27847/1‑149 (MQ=255)
                                                               gAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGat                                                                    <  1:170303/148‑1 (MQ=255)
                                                                    ttGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATggg                                                               <  8:27847/148‑1 (MQ=255)
                                                                     tGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGc                                                              >  1:348045/1‑148 (MQ=255)
                                                                     tGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGc                                                              >  5:360379/1‑148 (MQ=255)
                                                                        gggTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAAc                                                          <  7:217235/149‑1 (MQ=255)
                                                                          gtggtTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAAccc                                                        >  6:178613/1‑149 (MQ=255)
                                                                             gtTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAg                                                      <  6:360379/148‑1 (MQ=255)
                                                                                aCCCGGAGACACTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGTCGTTTGCCGATATGGGCGAACCCAGGCcg                                                  >  4:291456/1‑149 (MQ=255)
                                                                                  ccGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGCGCAGTGCCGCGTACATGCCAAGGTGAGTGCCATGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcg                                                >  6:299546/1‑149 (MQ=255)
                                                                                  ccGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcg                                                >  1:286094/1‑149 (MQ=255)
                                                                                   cGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcgc                                               <  2:348045/149‑1 (MQ=255)
                                                                                          ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                        >  1:379267/1‑149 (MQ=255)
                                                                                          ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                        >  4:92581/1‑149 (MQ=255)
                                                                                           cTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCAGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGAc                                       <  7:41945/149‑1 (MQ=255)
                                                                                            ttCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGAAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGAcc                                      >  8:91702/1‑149 (MQ=255)
                                                                                                 gAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTCGGCACGCGTGGCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGgga                                 <  8:75536/149‑1 (MQ=255)
                                                                                                 gAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTgg                                   <  5:178613/147‑1 (MQ=255)
                                                                                                         gtTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGa                         >  8:343517/1‑149 (MQ=255)
                                                                                                            gCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                   <  6:147206/72‑1 (MQ=255)
                                                                                                            gCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                   >  5:147206/1‑72 (MQ=255)
                                                                                                            gCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                       <  5:339036/148‑1 (MQ=255)
                                                                                                            gCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTg                      >  8:172908/1‑149 (MQ=255)
                                                                                                            gCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTg                      >  6:343121/1‑149 (MQ=255)
                                                                                                             cTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGc                     <  8:115001/149‑1 (MQ=255)
                                                                                                              tgtgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGcc                    >  5:175558/1‑149 (MQ=255)
                                                                                                                tgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCg                   <  5:156990/148‑1 (MQ=255)
                                                                                                                 gCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGt                 >  7:39077/1‑149 (MQ=255)
                                                                                                                  cAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGCGCCGATTGCCGGTg                >  2:186442/1‑149 (MQ=255)
                                                                                                                  cAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTg                <  7:172908/149‑1 (MQ=255)
                                                                                                                  aaacacGCAGTCCCAGTCCGTGCCTGTAGCCACGCGTGTCGAGCAGTGCCGCGTACATACCAACGTGACTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACAAAGGCCGCGCGTGATGACCTGGGAGGAGACGATTGCCGGTg                >  4:26845/6‑149 (MQ=255)
                                                                                                                   aaGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTgg               <  7:343517/149‑1 (MQ=255)
                                                                                                                    aGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATgg                                                                <  4:277622/99‑1 (MQ=255)
                                                                                                                    aGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGGGATGACCTGGGAGGAGCCGATTGCCGGTggg              >  7:54632/1‑149 (MQ=255)
                                                                                                                        gCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGggagga                              >  2:353150/1‑129 (MQ=255)
                                                                                                                        gCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGggagga                              <  1:353150/129‑1 (MQ=255)
                                                                                                                            tCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcg                                                >  4:41607/1‑107 (MQ=255)
                                                                                                                            tCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcg                                                <  3:41607/107‑1 (MQ=255)
                                                                                                                              ccAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc     >  7:35326/1‑148 (MQ=255)
                                                                                                                              ccAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACg    >  5:295505/1‑149 (MQ=255)
                                                                                                                              ccAGTGCGTGCCGGTAGGCACGGGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCATTGCTGGCTTCGGCGTTTGGCGATATGGGCGATCCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc     <  5:299546/148‑1 (MQ=255)
                                                                                                                                cacgcgtgcCTACAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAg  >  2:246209/4‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                        
GCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAG  >  NC_002947/2310909‑2311185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑