Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,318,661 A→G 5.2% A301A (GCA→GCG)  PP_2039 → acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,318,6610AG 5.2% 232.3 / 2.1 77A301A (GCA→GCG) PP_2039acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (43/30);  new base G (2/2);  total (45/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

CCACCTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCTGCTGC  >  NC_002947/2318519‑2318809
                                                                                                                                              |                                                                                                                                                    
ccACCTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACgcag                                                                                                                                                 >  4:327017/1‑148 (MQ=255)
   ccTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCGTGCAGCATg                                                                                                                                             <  1:105672/149‑1 (MQ=255)
   ccTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATg                                                                                                                                             <  3:281745/149‑1 (MQ=255)
   ccTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATg                                                                                                                                             <  8:112449/149‑1 (MQ=255)
   ccTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATg                                                                                                                                             <  3:327017/149‑1 (MQ=255)
                        tGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGCGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGt                                                                                                                         >  6:305935/1‑148 (MQ=255)
                         ggCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTg                                                                                                                        >  7:98972/1‑148 (MQ=255)
                          gCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGaa                                                                                                                      >  4:340034/1‑149 (MQ=255)
                                 aCCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACgc                                                                                                                                                   <  6:331989/113‑1 (MQ=255)
                                 aCCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACgc                                                                                                                                                   >  5:331989/1‑113 (MQ=255)
                                     ctcGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCcggcg                                                                                                           >  1:179660/1‑149 (MQ=255)
                                       cgatcgaCTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCcggcgg                                                                                                          >  6:370659/1‑148 (MQ=255)
                                         atcgaCTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCaa                                                                                                       >  6:287723/1‑149 (MQ=255)
                                             aCTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGTCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATc                                                                                                   >  4:18183/1‑149 (MQ=255)
                                             aCTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCGTGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATc                                                                                                   >  3:28889/1‑149 (MQ=255)
                                                  gCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCAc                                                                                              >  8:86117/1‑149 (MQ=255)
                                                     cGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCAcc                                                                                             >  5:12006/1‑147 (MQ=255)
                                                      gCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAg                                                                                          <  5:287723/149‑1 (MQ=255)
                                                       cACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATc                                                                                                   >  7:241776/1‑139 (MQ=255)
                                                       cACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATc                                                                                                   <  8:241776/139‑1 (MQ=255)
                                                        aCGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGc                                                                                        <  5:370659/149‑1 (MQ=255)
                                                          gcgcAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGccc                                                                                      >  6:168916/1‑149 (MQ=255)
                                                            gcAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGcccc                                                                                     >  4:158061/1‑148 (MQ=255)
                                                              aaGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCGTGCAGCATgcag                                                                                                                                          >  7:12255/1‑90 (MQ=255)
                                                              aaGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCGTGCAGCATgcag                                                                                                                                          <  8:12255/93‑4 (MQ=255)
                                                              aaGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGAt                                                                                  <  6:113621/149‑1 (MQ=255)
                                                               aGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGccc                                                                                      >  5:242160/1‑144 (MQ=255)
                                                               aGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGccc                                                                                      <  6:242160/144‑1 (MQ=255)
                                                                gCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGAtgt                                                                                <  3:340034/149‑1 (MQ=255)
                                                                   gTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGc                                                                              >  1:134196/1‑148 (MQ=255)
                                                                          cAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGtt                                                                      <  4:6128/149‑1 (MQ=255)
                                                                          cAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGtt                                                                      >  3:222780/1‑149 (MQ=255)
                                                                            gCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCcggc                                                                                                            >  6:120469/1‑109 (MQ=255)
                                                                            gCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCcggc                                                                                                            <  5:120469/109‑1 (MQ=255)
                                                                             ccTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGt                                                                   >  8:357874/1‑149 (MQ=255)
                                                                                 tCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTc                                                                <  7:86117/148‑1 (MQ=255)
                                                                                     cATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGgaga                                                           >  7:34504/1‑149 (MQ=255)
                                                                                             aGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGAt                                                                                  <  8:67321/118‑1 (MQ=255)
                                                                                             aGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTc                                                   <  8:34504/149‑1 (MQ=255)
                                                                                              gTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTcc                                                  >  7:285743/1‑149 (MQ=255)
                                                                                                   tCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGa                                                                                                                   >  4:299304/1‑79 (MQ=255)
                                                                                                   tCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGa                                                                                                                   <  3:299304/79‑1 (MQ=255)
                                                                                                     aaCCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGc                                           <  5:305935/149‑1 (MQ=255)
                                                                                                      aCCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACg                                                         <  2:75218/134‑1 (MQ=255)
                                                                                                      aCCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACg                                                         >  1:75218/1‑134 (MQ=255)
                                                                                                      aCCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGcc                                          >  2:166673/1‑149 (MQ=255)
                                                                                                       ccATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAAAATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGcc                                          >  2:70344/1‑148 (MQ=255)
                                                                                                       ccATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACACCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGcc                                          >  2:32191/1‑148 (MQ=255)
                                                                                                        cATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGa                                        <  6:12006/149‑1 (MQ=255)
                                                                                                             gATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATcgcg                                   >  1:4099/1‑149 (MQ=255)
                                                                                                             gATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATcgcg                                   >  1:147843/1‑149 (MQ=255)
                                                                                                              aTCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCgcgc                                  >  6:178719/1‑149 (MQ=255)
                                                                                                               tCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGcc                                 >  1:19915/1‑149 (MQ=255)
                                                                                                               tCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGcc                                 >  7:154475/1‑149 (MQ=255)
                                                                                                                 gCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGt                               >  3:269749/1‑149 (MQ=255)
                                                                                                                  ccGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTg                              >  6:110307/1‑149 (MQ=255)
                                                                                                                    gACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCa                            <  4:222780/149‑1 (MQ=255)
                                                                                                                       aTGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGa                          >  1:233011/1‑148 (MQ=255)
                                                                                                                        tGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATa                        >  2:29701/1‑149 (MQ=255)
                                                                                                                         ggCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATa                        >  3:79793/1‑148 (MQ=255)
                                                                                                                          gCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATaca                      >  3:176939/1‑149 (MQ=255)
                                                                                                                           ccACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTc                                                                >  8:314345/1‑106 (MQ=255)
                                                                                                                           ccACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTc                                                                <  7:314345/106‑1 (MQ=255)
                                                                                                                             aCCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACAcgg                   <  3:18183/149‑1 (MQ=255)
                                                                                                                              ccGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACAcggc                  <  5:168916/149‑1 (MQ=255)
                                                                                                                                   cTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCTCATCGCCGATCGCGCCGTGCAGATACACGGCTGTGc             <  2:4099/149‑1 (MQ=255)
                                                                                                                                   cTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGc             <  2:179660/149‑1 (MQ=255)
                                                                                                                                    accATGCTGCGTGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGcc            >  3:109056/3‑149 (MQ=255)
                                                                                                                                      cATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCgg          <  5:110307/149‑1 (MQ=255)
                                                                                                                                       aTGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCggg         <  2:233011/149‑1 (MQ=255)
                                                                                                                                        tgctgcACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGa                          >  6:115449/1‑131 (MQ=255)
                                                                                                                                        tgctgcACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGa                          <  5:115449/131‑1 (MQ=255)
                                                                                                                                         gctgcACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGgct       >  3:367594/1‑149 (MQ=255)
                                                                                                                                          ctgcACGCAGCATGGTCTACACCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCCCGCCGTGCAGATACACGGCGGTGCCGGgct       <  1:32191/148‑1 (MQ=255)
                                                                                                                                           tgcACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACAcggc                  <  4:45141/136‑1 (MQ=255)
                                                                                                                                           tgcACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACAcggc                  >  3:45141/1‑136 (MQ=255)
                                                                                                                                           tgcACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGgctgc     >  6:71925/1‑149 (MQ=255)
                                                                                                                                              aCGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCtgctgc  >  7:239071/1‑149 (MQ=255)
                                                                                                                                              aCGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCtgctgc  <  2:19915/149‑1 (MQ=255)
                                                                                                                                              aCGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCtgctgc  <  8:285743/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CCACCTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCTGCTGC  >  NC_002947/2318519‑2318809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑