Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,334,843 C→T 10.0% Q538Q (CAG→CAA) ‡ PP_2052 ← bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,334,8430CT10.0% 165.5 / 6.9 60Q538Q (CAG→CAA) ‡PP_2052bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base T (3/3);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCG  >  NC_002947/2334699‑2334990
                                                                                                                                                |                                                                                                                                                   
cGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGc                                                                                                                                                 >  4:16725/1‑149 (MQ=255)
 gAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGcc                                                                                                                                                >  7:294173/1‑149 (MQ=255)
 gAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGcc                                                                                                                                                <  8:66152/149‑1 (MQ=255)
     ttttGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGAcc                                                                                                                                            <  2:112480/149‑1 (MQ=255)
             cgcTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATg                                                                                                                                    <  3:73165/149‑1 (MQ=255)
                tCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGACACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTAACCCGCGCATCGCCCATGCCCTGGCAGACCCAGGCATGGcc                                                                                                                                 >  2:107309/1‑149 (MQ=255)
                 cGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                   >  7:285717/1‑146 (MQ=255)
                 cGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                   <  8:285717/146‑1 (MQ=255)
                  gagaAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGcc                                                                                                                                 >  5:365085/1‑147 (MQ=255)
                   agaAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCcag                                                                                                                              >  7:354646/1‑149 (MQ=255)
                                   aGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAg                                                                                                              <  6:365085/149‑1 (MQ=255)
                                   aGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAg                                                                                                              <  8:294173/149‑1 (MQ=255)
                                   aGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCAGCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAg                                                                                                              <  6:189024/149‑1 (MQ=255)
                                           acaaccTCGGACATCTGTGGGTATTAGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGAGTACCAGGCCACAAt                                                                                                      >  2:310522/4‑149 (MQ=255)
                                            ccaccTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATg                                                                                                     >  4:341567/1‑149 (MQ=255)
                                                    gcgaTCTGTGGGTAGTCGGCGAGGAGTGCTGGGGCGACCTCCTGGCGAATCAGGCGCTCGGCCATTTCACTCACCGGAGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCTCAGCAGTCTGGCGTACCAGGCCACAATGTCGTGCgg                                                                                             <  8:244282/146‑1 (MQ=255)
                                                     ccATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCggg                                                                                            >  7:72164/1‑149 (MQ=255)
                                                           gtgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTc                                                                                      >  6:342276/1‑149 (MQ=255)
                                                            tgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGTCGTACCAGGCCACAATGGCGTGCGGGCCGTTc                                                                                      <  3:16725/148‑1 (MQ=255)
                                                            tgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTc                                                                                      <  4:105545/148‑1 (MQ=255)
                                                             gggTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCTCTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCca                                                                                    >  1:261013/1‑149 (MQ=255)
                                                                     ggCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGGCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGt                                                                            >  4:147737/1‑149 (MQ=255)
                                                                       ccaccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCTGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCTTGCGGGCCGTTCCACAGGCTGTTc                                                                          <  1:310522/149‑1 (MQ=255)
                                                                       ccaccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTc                                                                          >  6:193969/1‑149 (MQ=255)
                                                                          ccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATggg                                                                                                                                  <  2:82225/90‑2 (MQ=37)
                                                                          ccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATggg                                                                                                                                  >  1:82225/1‑89 (MQ=37)
                                                                                 gggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                >  3:57730/1‑149 (MQ=255)
                                                                                  ggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatctg                                                               >  7:354431/1‑149 (MQ=255)
                                                                                    cgcgACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTGTTGATgatctgga                                                             <  1:107309/149‑1 (MQ=255)
                                                                                         cctcctGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGatctggatctgg                                                        <  8:354431/149‑1 (MQ=255)
                                                                                           tcctGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTggg                                                       >  7:25495/1‑148 (MQ=255)
                                                                                              tGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCCCAGGCGGTTCTTGATGATCTGGATCTGGGCAAt                                                   >  1:322725/1‑149 (MQ=255)
                                                                                                gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGGCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAAt                                                   <  3:147737/147‑1 (MQ=255)
                                                                                                gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAAt                                                   <  8:72164/147‑1 (MQ=255)
                                                                                                gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGt                                                 <  8:25495/149‑1 (MQ=255)
                                                                                                  gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCt                                               >  6:172575/1‑149 (MQ=255)
                                                                                                  gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCt                                               <  2:127877/149‑1 (MQ=255)
                                                                                                   aaTCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTg                                              <  4:205177/149‑1 (MQ=255)
                                                                                                     tCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGc                                            >  8:350580/1‑149 (MQ=255)
                                                                                                     tCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGc                                            >  3:91867/1‑149 (MQ=255)
                                                                                                       aGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCa                                           <  4:57730/148‑1 (MQ=255)
                                                                                                       aGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCa                                           <  3:341567/148‑1 (MQ=255)
                                                                                                           gcTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTc                                      <  4:91867/149‑1 (MQ=255)
                                                                                                               ggCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCtt                                    <  5:172575/147‑1 (MQ=255)
                                                                                                               ggCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTc                                   <  5:342276/148‑1 (MQ=255)
                                                                                                               ggCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGACGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCAGGGCAATGTCTCGCACAGTCTTCa                                  >  6:211097/1‑149 (MQ=255)
                                                                                                                    gTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGc                                                      >  2:373517/1‑124 (MQ=255)
                                                                                                                         ctcacCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAg                        <  8:354646/149‑1 (MQ=255)
                                                                                                                          tcacCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAgg                       >  3:324945/1‑149 (MQ=255)
                                                                                                                          tcacCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCAAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGGTCCACAGGCGGGTCTTGATGCACTGGATCTCGGCAATGTCGGGCACTGTCTTCCACTTGCGCAgg                       >  8:245745/1‑149 (MQ=255)
                                                                                                                              ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGatctggatctgg                                                        >  7:148316/1‑112 (MQ=255)
                                                                                                                              ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGatctggatctgg                                                        <  8:148316/112‑1 (MQ=255)
                                                                                                                              ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCa                                                     <  7:144600/115‑1 (MQ=255)
                                                                                                                              ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCa                                                     >  8:144600/1‑115 (MQ=255)
                                                                                                                                        gccATGCCTGGGTCGGCCAGGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAGTGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                >  6:374522/2‑94 (MQ=37)
                                                                                                                                        gccATGCCTGGGTCGGCCAGGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAGTGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                <  5:374522/93‑1 (MQ=37)
                                                                                                                                        cccATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTTGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTTTCTTCACCTGGCGCAGGCGTTCTAGCAGATc         <  2:269214/149‑1 (MQ=255)
                                                                                                                                         ccATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCg        >  2:20660/1‑149 (MQ=255)
                                                                                                                                           aTGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCCCCTGGCGCAGGCGTTCGAGCAGATCGCt      >  6:340524/1‑149 (MQ=255)
                                                                                                                                            tGCCCTGGCCTACCCAGGCATGGCCCAGCATGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTg     <  5:211097/149‑1 (MQ=255)
                                                                                                                                              cccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGcc   <  7:350580/149‑1 (MQ=255)
                                                                                                                                               ccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCcg  >  1:168027/1‑149 (MQ=255)
                                                                                                                                               ccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCcg  >  6:147680/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCG  >  NC_002947/2334699‑2334990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑