Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 227,872 G→A 6.6% R233H (CGC→CAC)  PP_0176 → putative efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947227,8720GA 6.6% 179.6 / 2.0 61R233H (CGC→CAC) PP_0176putative efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base A (2/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

ACAGCGACACCTACCTGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTCGTGAATGGCTCAAAGGCACCG  >  NC_002947/227725‑228018
                                                                                                                                                   |                                                                                                                                                  
aCAGCGACACCTACCTGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCGGGCCCGCATACCCTGAAGAACGCCcgc                                                                                                                                                   >  4:35320/1‑149 (MQ=255)
 cAGCGACACCTACCTGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCcg                                                                                                                                                    <  1:245424/147‑1 (MQ=255)
     gACACCTACCTGGCCCTCGATGCCAGCGCTGACATGGTCGGCTGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGTAGGTTCCGGCCGGCATACCCTGAAGAACGCGCGCGAAtt                                                                                                                                              <  7:315504/149‑1 (MQ=255)
       cacCTACCTGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGCCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGaa                                                                                                                                                <  3:337469/145‑1 (MQ=255)
       cacCTACCTGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGCCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGaa                                                                                                                                                >  4:337469/1‑145 (MQ=255)
        acctacctGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCg                                                                                                                                           <  3:176653/149‑1 (MQ=255)
             cctGGCGCGCGTTGCCAGGGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCcgc                                                                                                                                                   <  4:155315/136‑1 (MQ=255)
             cctGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCcgc                                                                                                                                                   <  4:149732/136‑1 (MQ=255)
             cctGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCcgc                                                                                                                                                   >  3:155315/1‑136 (MQ=255)
             cctGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCcgc                                                                                                                                                   >  3:149732/1‑136 (MQ=255)
               tGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCg                                                                                                                                     >  3:116523/1‑148 (MQ=255)
                 gCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGTCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCgg                                                                                                                                    <  1:371186/147‑1 (MQ=255)
                   ccGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGgatga                                                                                                                                >  6:378048/1‑149 (MQ=255)
                    cgcgATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCACGCAATTCGCGGGCg                                                                                                                                     >  1:352861/1‑143 (MQ=255)
                    cgcgATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCACGCAATTCGCGGGCg                                                                                                                                     <  2:352861/143‑1 (MQ=255)
                     gcgATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATc                                                                                                                              <  6:65090/149‑1 (MQ=255)
                     gcgATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATc                                                                                                                              <  4:116523/149‑1 (MQ=255)
                     gcgATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGAAGACACAGAAGAAAGCCCCCGAAATTCGTGGGCGgctgat                                                                                                                               >  1:126556/1‑148 (MQ=255)
                         tGCCAGCGCTGACAAGGGCGGCGGTATGCGCGTGGCGATGTTCACTACCTTCAACTCGCTGGAGCTGATGATCTCTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATc                                                                                                                          <  4:375422/149‑1 (MQ=255)
                            cAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATctgc                                                                                                                       >  1:349922/1‑149 (MQ=255)
                                  tGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCCCGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCgg                                                                                                                 >  6:234408/1‑149 (MQ=255)
                                  tGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCgg                                                                                                                 >  6:239803/1‑149 (MQ=255)
                                    aCAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGt                                                                                                                >  2:46549/1‑148 (MQ=255)
                                          tCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGAc                                                                                                         <  2:349922/149‑1 (MQ=255)
                                              cggTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGccc                                                                                                     <  2:281979/149‑1 (MQ=255)
                                                 tATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTcg                                                                                                  <  1:347052/149‑1 (MQ=255)
                                                      gcgGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCTAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGa                                                                                                              <  3:60385/132‑1 (MQ=255)
                                                      gcgGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGa                                                                                                              >  4:60385/1‑132 (MQ=255)
                                                      gcgGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCgacgac                                                                                             >  5:57973/1‑149 (MQ=255)
                                                          ggCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCAc                                                                                         <  5:378048/149‑1 (MQ=255)
                                                              aTGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTgg                                                                                      >  3:187915/1‑148 (MQ=255)
                                                                ggTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAg                                                                                   >  4:49952/1‑149 (MQ=255)
                                                                 gTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGgat                                                                                                                                  >  5:88600/1‑101 (MQ=255)
                                                                 gTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGgat                                                                                                                                  <  6:88600/101‑1 (MQ=255)
                                                                    aCTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCt                                                                               <  1:33346/149‑1 (MQ=255)
                                                                       aCCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCt                                                                               <  7:230961/146‑1 (MQ=255)
                                                                       aCCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCt                                                                               >  8:230961/1‑146 (MQ=255)
                                                                         cTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAg                                                                          >  4:320354/1‑149 (MQ=255)
                                                                             aaCTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGcc                                                                       <  3:35320/148‑1 (MQ=255)
                                                                             aaCTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGcc                                                                       <  7:297388/148‑1 (MQ=255)
                                                                              aCTCGCTGGAGCTGATGATCTGTCAGCTCGCCGACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCcgc                                                                     <  1:239447/149‑1 (MQ=255)
                                                                                  gCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGccc                                                                 <  1:46549/149‑1 (MQ=255)
                                                                                              gatCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTc                                                      <  2:126556/148‑1 (MQ=255)
                                                                                               atCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCg                                                     <  6:253437/148‑1 (MQ=255)
                                                                                                      aGCTCGCCCACGAAGGTGCCAGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTcga                                                                                                 <  1:218398/97‑1 (MQ=255)
                                                                                                      aGCTCGCCCACGAAGGTGCCAGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTcga                                                                                                 >  2:218398/1‑97 (MQ=255)
                                                                                                          cGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAAc                                         >  6:346390/1‑149 (MQ=255)
                                                                                                           gCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAAcc                                        >  7:47182/1‑149 (MQ=255)
                                                                                                              cACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGt                                     <  4:187915/149‑1 (MQ=255)
                                                                                                                    ggTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTgg                                >  7:180300/1‑148 (MQ=255)
                                                                                                                       gCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGc                            <  3:49952/149‑1 (MQ=255)
                                                                                                                           gCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGc                                                    <  2:335648/121‑1 (MQ=255)
                                                                                                                           gCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGc                                                    >  1:335648/1‑121 (MQ=255)
                                                                                                                            cccGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTc                       >  4:272108/1‑149 (MQ=255)
                                                                                                                            cccGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTc                       <  5:239803/149‑1 (MQ=255)
                                                                                                                            cccGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTc                       <  5:234408/149‑1 (MQ=255)
                                                                                                                                      cTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGaa                              >  1:29865/1‑132 (MQ=255)
                                                                                                                                      cTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGGGCTGGaa                              <  2:29865/132‑1 (MQ=255)
                                                                                                                                        gaagaaCGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTCGTGAATGGCTc            >  7:68700/1‑148 (MQ=255)
                                                                                                                                         aagaaCGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTCg                      >  6:251665/1‑137 (MQ=255)
                                                                                                                                         aagaaCGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTCg                      <  5:251665/137‑1 (MQ=255)
                                                                                                                                           atccGCCCACGCAATTCGCGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGcc                                                            >  2:149645/4‑97 (MQ=255)
                                                                                                                                           atccGCCCACGCAATTCGCGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGcc                                                            <  1:149645/94‑1 (MQ=255)
                                                                                                                                             aCGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTCGTGAATGGCTCAAAGGc      <  6:57973/149‑1 (MQ=255)
                                                                                                                                               gCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTCGTGAATGGCTCAAAGGCAc    >  3:236590/1‑149 (MQ=255)
                                                                                                                                                cccGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTCGTGAATGGCTCAAAGGCAcc   >  1:345549/1‑149 (MQ=255)
                                                                                                                                                 ccGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTCGTGAATGGCTCAAAGGCAccg  >  2:355937/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACAGCGACACCTACCTGGCCCGCGATGCCAGCGCTGACAAGGTCGGCGGTATGCGCGGGGCGATGGTCACTACCTTCAACTCGCTGGAGCTGATGATCGGTCAGCTCGCCCACGAAGGTGCCGGCCCGCATACCCTGAAGAACGCCCGCGAATTGCGTGGGCGGATGATCCATCTGCTGCCGGTGATCGACGCCCTCGACGACGCACTGGTAGCCCTGGAAGGCCGCGCCCCGGCCCAGTTCGCCCAGCTTCAACCCGTGCTGGAAGCCGCTCGTGAATGGCTCAAAGGCACCG  >  NC_002947/227725‑228018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑