Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,334,850 A→G 9.5% V536A (GTC→GCC)  PP_2052 ← bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,334,8500AG 9.5% 165.9 / 7.2 63V536A (GTC→GCC) PP_2052bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (28/29);  new base G (3/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

TTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTC  >  NC_002947/2334704‑2334995
                                                                                                                                                  |                                                                                                                                                 
ttttGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGAcc                                                                                                                                                 <  2:112480/149‑1 (MQ=255)
        cgcTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATg                                                                                                                                         <  3:73165/149‑1 (MQ=255)
           tCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGACACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTAACCCGCGCATCGCCCATGCCCTGGCAGACCCAGGCATGGcc                                                                                                                                      >  2:107309/1‑149 (MQ=255)
            cGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                        <  8:285717/146‑1 (MQ=255)
            cGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                        >  7:285717/1‑146 (MQ=255)
             gagaAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGcc                                                                                                                                      >  5:365085/1‑147 (MQ=255)
              agaAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCcag                                                                                                                                   >  7:354646/1‑149 (MQ=255)
                              aGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAg                                                                                                                   <  8:294173/149‑1 (MQ=255)
                              aGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAg                                                                                                                   <  6:365085/149‑1 (MQ=255)
                              aGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCAGCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAg                                                                                                                   <  6:189024/149‑1 (MQ=255)
                                      acaaccTCGGACATCTGTGGGTATTAGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGAGTACCAGGCCACAAt                                                                                                           >  2:310522/4‑149 (MQ=255)
                                       ccaccTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATg                                                                                                          >  4:341567/1‑149 (MQ=255)
                                               gcgaTCTGTGGGTAGTCGGCGAGGAGTGCTGGGGCGACCTCCTGGCGAATCAGGCGCTCGGCCATTTCACTCACCGGAGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCTCAGCAGTCTGGCGTACCAGGCCACAATGTCGTGCgg                                                                                                  <  8:244282/146‑1 (MQ=255)
                                                ccATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCggg                                                                                                 >  7:72164/1‑149 (MQ=255)
                                                      gtgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTc                                                                                           >  6:342276/1‑149 (MQ=255)
                                                       tgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGTCGTACCAGGCCACAATGGCGTGCGGGCCGTTc                                                                                           <  3:16725/148‑1 (MQ=255)
                                                       tgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTc                                                                                           <  4:105545/148‑1 (MQ=255)
                                                        gggTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCTCTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCca                                                                                         >  1:261013/1‑149 (MQ=255)
                                                                ggCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGGCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGt                                                                                 >  4:147737/1‑149 (MQ=255)
                                                                  ccaccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCTGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCTTGCGGGCCGTTCCACAGGCTGTTc                                                                               <  1:310522/149‑1 (MQ=255)
                                                                  ccaccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTc                                                                               >  6:193969/1‑149 (MQ=255)
                                                                     ccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATggg                                                                                                                                       <  2:82225/90‑2 (MQ=37)
                                                                     ccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATggg                                                                                                                                       >  1:82225/1‑89 (MQ=37)
                                                                            gggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                     >  3:57730/1‑149 (MQ=255)
                                                                             ggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatctg                                                                    >  7:354431/1‑149 (MQ=255)
                                                                               cgcgACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTGTTGATgatctgga                                                                  <  1:107309/149‑1 (MQ=255)
                                                                                    cctcctGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGatctggatctgg                                                             <  8:354431/149‑1 (MQ=255)
                                                                                      tcctGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTggg                                                            >  7:25495/1‑148 (MQ=255)
                                                                                         tGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCCCAGGCGGTTCTTGATGATCTGGATCTGGGCAAt                                                        >  1:322725/1‑149 (MQ=255)
                                                                                           gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGGCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAAt                                                        <  3:147737/147‑1 (MQ=255)
                                                                                           gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAAt                                                        <  8:72164/147‑1 (MQ=255)
                                                                                           gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGt                                                      <  8:25495/149‑1 (MQ=255)
                                                                                             gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCt                                                    <  2:127877/149‑1 (MQ=255)
                                                                                             gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCt                                                    >  6:172575/1‑149 (MQ=255)
                                                                                              aaTCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTg                                                   <  4:205177/149‑1 (MQ=255)
                                                                                                tCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGc                                                 >  3:91867/1‑149 (MQ=255)
                                                                                                tCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGc                                                 >  8:350580/1‑149 (MQ=255)
                                                                                                  aGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCa                                                <  3:341567/148‑1 (MQ=255)
                                                                                                  aGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCa                                                <  4:57730/148‑1 (MQ=255)
                                                                                                      gcTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTc                                           <  4:91867/149‑1 (MQ=255)
                                                                                                          ggCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCtt                                         <  5:172575/147‑1 (MQ=255)
                                                                                                          ggCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTc                                        <  5:342276/148‑1 (MQ=255)
                                                                                                          ggCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGACGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCAGGGCAATGTCTCGCACAGTCTTCa                                       >  6:211097/1‑149 (MQ=255)
                                                                                                               gTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGc                                                           >  2:373517/1‑124 (MQ=255)
                                                                                                                    ctcacCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAg                             <  8:354646/149‑1 (MQ=255)
                                                                                                                     tcacCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAgg                            >  3:324945/1‑149 (MQ=255)
                                                                                                                     tcacCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCAAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGGTCCACAGGCGGGTCTTGATGCACTGGATCTCGGCAATGTCGGGCACTGTCTTCCACTTGCGCAgg                            >  8:245745/1‑149 (MQ=255)
                                                                                                                         ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGatctggatctgg                                                             >  7:148316/1‑112 (MQ=255)
                                                                                                                         ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGatctggatctgg                                                             <  8:148316/112‑1 (MQ=255)
                                                                                                                         ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCa                                                          <  7:144600/115‑1 (MQ=255)
                                                                                                                         ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCa                                                          >  8:144600/1‑115 (MQ=255)
                                                                                                                                   gccATGCCTGGGTCGGCCAGGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAGTGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                     >  6:374522/2‑94 (MQ=37)
                                                                                                                                   gccATGCCTGGGTCGGCCAGGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAGTGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                     <  5:374522/93‑1 (MQ=37)
                                                                                                                                   cccATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTTGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTTTCTTCACCTGGCGCAGGCGTTCTAGCAGATc              <  2:269214/149‑1 (MQ=255)
                                                                                                                                    ccATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCg             >  2:20660/1‑149 (MQ=255)
                                                                                                                                      aTGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCCCCTGGCGCAGGCGTTCGAGCAGATCGCt           >  6:340524/1‑149 (MQ=255)
                                                                                                                                       tGCCCTGGCCTACCCAGGCATGGCCCAGCATGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTg          <  5:211097/149‑1 (MQ=255)
                                                                                                                                         cccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGcc        <  7:350580/149‑1 (MQ=255)
                                                                                                                                          ccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCcg       >  1:168027/1‑149 (MQ=255)
                                                                                                                                          ccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCcg       >  6:147680/1‑149 (MQ=255)
                                                                                                                                              gCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCGATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGcc        <  6:345311/144‑1 (MQ=255)
                                                                                                                                              gCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCGATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGcc        >  5:345311/1‑144 (MQ=255)
                                                                                                                                               ccGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTc  <  4:324945/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTC  >  NC_002947/2334704‑2334995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑