Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,334,854 A→G 9.4% W535R (TGG→CGG) ‡ PP_2052 ← bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,334,8540AG 9.4% 175.8 / 7.0 64W535R (TGG→CGG) ‡PP_2052bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base G (3/3);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

CGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTCGGCGTAA  >  NC_002947/2334712‑2335002
                                                                                                                                              |                                                                                                                                                    
cgcTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATg                                                                                                                                                <  3:73165/149‑1 (MQ=255)
   tCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGACACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTAACCCGCGCATCGCCCATGCCCTGGCAGACCCAGGCATGGcc                                                                                                                                             >  2:107309/1‑149 (MQ=255)
    cGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                               >  7:285717/1‑146 (MQ=255)
    cGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                               <  8:285717/146‑1 (MQ=255)
     gagaAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGcc                                                                                                                                             >  5:365085/1‑147 (MQ=255)
      agaAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCcag                                                                                                                                          >  7:354646/1‑149 (MQ=255)
                      aGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAg                                                                                                                          <  6:365085/149‑1 (MQ=255)
                      aGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAg                                                                                                                          <  8:294173/149‑1 (MQ=255)
                      aGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCAGCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAg                                                                                                                          <  6:189024/149‑1 (MQ=255)
                              acaaccTCGGACATCTGTGGGTATTAGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGAGTACCAGGCCACAAt                                                                                                                  >  2:310522/4‑149 (MQ=255)
                               ccaccTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATg                                                                                                                 >  4:341567/1‑149 (MQ=255)
                                       gcgaTCTGTGGGTAGTCGGCGAGGAGTGCTGGGGCGACCTCCTGGCGAATCAGGCGCTCGGCCATTTCACTCACCGGAGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCTCAGCAGTCTGGCGTACCAGGCCACAATGTCGTGCgg                                                                                                         <  8:244282/146‑1 (MQ=255)
                                        ccATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCggg                                                                                                        >  7:72164/1‑149 (MQ=255)
                                              gtgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTc                                                                                                  >  6:342276/1‑149 (MQ=255)
                                               tgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGTCGTACCAGGCCACAATGGCGTGCGGGCCGTTc                                                                                                  <  3:16725/148‑1 (MQ=255)
                                               tgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTc                                                                                                  <  4:105545/148‑1 (MQ=255)
                                                gggTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCTCTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCca                                                                                                >  1:261013/1‑149 (MQ=255)
                                                        ggCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGGCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGt                                                                                        >  4:147737/1‑149 (MQ=255)
                                                          ccaccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCTGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCTTGCGGGCCGTTCCACAGGCTGTTc                                                                                      <  1:310522/149‑1 (MQ=255)
                                                          ccaccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTc                                                                                      >  6:193969/1‑149 (MQ=255)
                                                             ccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATggg                                                                                                                                              <  2:82225/90‑2 (MQ=37)
                                                             ccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCTGGGTCGGCCAGGGCATggg                                                                                                                                              >  1:82225/1‑89 (MQ=37)
                                                                    gggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                            >  3:57730/1‑149 (MQ=255)
                                                                     ggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatctg                                                                           >  7:354431/1‑149 (MQ=255)
                                                                       cgcgACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTGTTGATgatctgga                                                                         <  1:107309/149‑1 (MQ=255)
                                                                            cctcctGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGatctggatctgg                                                                    <  8:354431/149‑1 (MQ=255)
                                                                              tcctGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTggg                                                                   >  7:25495/1‑148 (MQ=255)
                                                                                 tGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCCCAGGCGGTTCTTGATGATCTGGATCTGGGCAAt                                                               >  1:322725/1‑149 (MQ=255)
                                                                                   gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGGCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAAt                                                               <  3:147737/147‑1 (MQ=255)
                                                                                   gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAAt                                                               <  8:72164/147‑1 (MQ=255)
                                                                                   gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGt                                                             <  8:25495/149‑1 (MQ=255)
                                                                                     gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCt                                                           <  2:127877/149‑1 (MQ=255)
                                                                                     gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCt                                                           >  6:172575/1‑149 (MQ=255)
                                                                                      aaTCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTg                                                          <  4:205177/149‑1 (MQ=255)
                                                                                        tCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGc                                                        >  8:350580/1‑149 (MQ=255)
                                                                                        tCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGc                                                        >  3:91867/1‑149 (MQ=255)
                                                                                          aGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCa                                                       <  3:341567/148‑1 (MQ=255)
                                                                                          aGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCa                                                       <  4:57730/148‑1 (MQ=255)
                                                                                              gcTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTc                                                  <  4:91867/149‑1 (MQ=255)
                                                                                                  ggCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCtt                                                <  5:172575/147‑1 (MQ=255)
                                                                                                  ggCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTc                                               <  5:342276/148‑1 (MQ=255)
                                                                                                  ggCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGACGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCAGGGCAATGTCTCGCACAGTCTTCa                                              >  6:211097/1‑149 (MQ=255)
                                                                                                       gTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGc                                                                  >  2:373517/1‑124 (MQ=255)
                                                                                                            ctcacCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAg                                    <  8:354646/149‑1 (MQ=255)
                                                                                                             tcacCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAgg                                   >  3:324945/1‑149 (MQ=255)
                                                                                                             tcacCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCAAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGGTCCACAGGCGGGTCTTGATGCACTGGATCTCGGCAATGTCGGGCACTGTCTTCCACTTGCGCAgg                                   >  8:245745/1‑149 (MQ=255)
                                                                                                                 ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGatctggatctgg                                                                    >  7:148316/1‑112 (MQ=255)
                                                                                                                 ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGatctggatctgg                                                                    <  8:148316/112‑1 (MQ=255)
                                                                                                                 ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCa                                                                 <  7:144600/115‑1 (MQ=255)
                                                                                                                 ccGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCa                                                                 >  8:144600/1‑115 (MQ=255)
                                                                                                                           gccATGCCTGGGTCGGCCAGGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAGTGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                            >  6:374522/2‑94 (MQ=37)
                                                                                                                           gccATGCCTGGGTCGGCCAGGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAGTGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                            <  5:374522/93‑1 (MQ=37)
                                                                                                                           cccATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTTGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTTTCTTCACCTGGCGCAGGCGTTCTAGCAGATc                     <  2:269214/149‑1 (MQ=255)
                                                                                                                            ccATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCg                    >  2:20660/1‑149 (MQ=255)
                                                                                                                              aTGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCCCCTGGCGCAGGCGTTCGAGCAGATCGCt                  >  6:340524/1‑149 (MQ=255)
                                                                                                                               tGCCCTGGCCTACCCAGGCATGGCCCAGCATGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTg                 <  5:211097/149‑1 (MQ=255)
                                                                                                                                 cccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGcc               <  7:350580/149‑1 (MQ=255)
                                                                                                                                  ccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCcg              >  6:147680/1‑149 (MQ=255)
                                                                                                                                  ccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCcg              >  1:168027/1‑149 (MQ=255)
                                                                                                                                      gCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCGATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGcc               >  5:345311/1‑144 (MQ=255)
                                                                                                                                      gCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCGATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGcc               <  6:345311/144‑1 (MQ=255)
                                                                                                                                       ccGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTc         <  4:324945/149‑1 (MQ=255)
                                                                                                                                            ccAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTCGGCg     >  7:86505/1‑148 (MQ=255)
                                                                                                                                            ccAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTCGGCg     >  1:169249/1‑148 (MQ=255)
                                                                                                                                              aGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTggg                                                                   <  8:146715/84‑1 (MQ=255)
                                                                                                                                              aGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTggg                                                                   >  7:146715/1‑84 (MQ=255)
                                                                                                                                              aGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTCGGCGTaa  <  5:193969/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTCGGCGTAA  >  NC_002947/2334712‑2335002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑