| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,338,201 | C→T | 6.5% | intergenic (‑140/‑7) | ybhD ← / → PP_2055 | DNA‑binding transcriptional regulator/isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,338,201 | 0 | C | T | 6.5% | 177.1 / 2.1 | 61 | intergenic (‑140/‑7) | ybhD/PP_2055 | DNA‑binding transcriptional regulator/isomerase |
| Reads supporting (aligned to +/- strand): ref base C (29/28); new base T (2/2); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-01 | |||||||||||
CATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTCATGGGCTCTGGCCATGAGCT > NC_002947/2338059‑2338341 | cATAAGAAACCTTCGATTTGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAAt < 1:281372/148‑1 (MQ=255) aTAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCa < 1:222333/149‑1 (MQ=255) aaGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCACTGTCAATCTTTTGATCACAGCATAAATGATGTCCATTGATCAAAAATAGTCCGAAATCAt > 3:221446/1‑148 (MQ=255) aGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATg < 7:213099/148‑1 (MQ=255) aaaCCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCGATTGATCAACAAGAGTGCGAAATCATGCaa > 2:218809/1‑149 (MQ=255) aaaCCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCaa > 2:224407/1‑149 (MQ=255) aaCCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAAc < 8:271002/149‑1 (MQ=255) ccTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACg > 6:125174/1‑148 (MQ=255) ttCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAAt > 3:135133/1‑149 (MQ=255) aTTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCt < 2:174606/149‑1 (MQ=255) gCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTTGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGc < 8:52502/148‑1 (MQ=255) aCTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCa > 5:57251/1‑128 (MQ=255) aCTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCa < 6:57251/128‑1 (MQ=255) aCTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCAt > 3:130697/1‑149 (MQ=255) cTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATg > 2:276336/1‑149 (MQ=255) tttATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATgc > 8:322394/1‑149 (MQ=255) cATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAACGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCgcggcg > 2:340182/1‑149 (MQ=255) aTGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGc > 6:129086/1‑118 (MQ=255) aTGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGc < 5:129086/118‑1 (MQ=255) aTGTATGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCgcggcg < 8:168594/148‑1 (MQ=255) taatctaaataatAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCt < 4:221446/141‑1 (MQ=255) tCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGTGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTa < 3:311175/142‑1 (MQ=255) tCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGTGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTa < 3:316360/142‑1 (MQ=255) tCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGTGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTa > 4:311175/1‑142 (MQ=255) tCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGTGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTa > 4:316360/1‑142 (MQ=255) cAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAgg > 7:303648/1‑149 (MQ=255) tACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGttcttc > 8:228794/1‑149 (MQ=255) cGATATTTGCATTGGATTGATTTATACATCCCTGTCACTCTTTTGCTAACAGCATCAATGATGTACATTGATCAACAAGATTGCTAAATAATGCAACGAATTCCTTGCGTACTCATGCGAGGCGGTACATCCAAAGGGCCTTTATTCCa > 7:314060/1‑149 (MQ=255) gCAATGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTgg > 6:79317/1‑148 (MQ=255) gattgattTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAACGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGAcctgc < 1:340182/149‑1 (MQ=255) attTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCgcggcg > 5:308301/1‑105 (MQ=255) attTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCgcggcg < 6:308301/105‑1 (MQ=255) atccatccCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAAtg > 8:300437/1‑149 (MQ=255) ccatccCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAAtgtg > 4:188736/1‑149 (MQ=255) catccCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGt > 8:24095/1‑100 (MQ=255) catccCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGt < 7:24095/100‑1 (MQ=255) catccCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAAtgtg < 7:322394/148‑1 (MQ=255) atccCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCAt < 8:350528/89‑1 (MQ=255) tccCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTc < 7:300437/149‑1 (MQ=255) cTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTGCCCCGCCTGGGCGCTGCCTGCCAATGTGGTCGAg > 8:337293/1‑149 (MQ=255) tGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAgc > 5:286881/1‑149 (MQ=255) aCTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGcga < 7:61499/149‑1 (MQ=255) aCTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGACGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGcga > 5:152770/1‑149 (MQ=255) ctctTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGcgac < 4:130697/149‑1 (MQ=255) ctTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGcgac > 6:199060/1‑147 (MQ=255) ttttGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAg > 3:251652/1‑149 (MQ=255) tGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGtt < 3:188736/148‑1 (MQ=255) cTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATg > 6:269929/1‑48 (MQ=255) cTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATg < 5:269929/48‑1 (MQ=255) cTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGc < 4:251652/148‑1 (MQ=255) acaGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGa < 5:79317/148‑1 (MQ=255) aaTGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTc < 1:276336/148‑1 (MQ=255) atgatgTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCAGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTCAt < 8:303648/149‑1 (MQ=255) cATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTCATGGGCTCTg > 5:334028/1‑149 (MQ=255) aTTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTCATGGGCTCTgg < 5:125174/149‑1 (MQ=255) aTTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTCATGGGCTCTgg > 3:61766/1‑149 (MQ=255) ttGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCGTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTCATGGGCTCTGGc > 2:320207/1‑149 (MQ=255) aTCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTCATGGGCTCTGGCCAt > 6:347678/1‑149 (MQ=255) aacaaGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTCATGGGCTCTGGCCATGa < 4:135133/148‑1 (MQ=255) caaGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTAATCAACCTCATGGGCTCTGGCCATGAGCt < 1:224407/149‑1 (MQ=255) caaGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTAATCAACCTCATGGGCTCTGGCCATGAGCt < 1:218809/149‑1 (MQ=255) | CATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTCCTTGCGTACTCATGCGCGGCGGTACCTCCAAAGGGCCGTTCTTCCACGCCTGGGACCTGCCTGCCAATGTGGTCGAGCGCGACGAGTTGCTGATCAACCTCATGGGCTCTGGCCATGAGCT > NC_002947/2338059‑2338341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |