Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,347,622 G→C 9.8% S308T (AGC→ACC)  PP_2064 → multidrug RND transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,347,6220GC 9.8% 118.4 / 2.5 40S308T (AGC→ACC) PP_2064multidrug RND transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base G (18/18);  new base C (2/2);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCCGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAGCCTGCAG  >  NC_002947/2347476‑2347770
                                                                                                                                                   |                                                                                                                                                    
gCAGCCGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGc                                                                                                                                                     <  3:327728/148‑1 (MQ=255)
   gCCGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCggg                                                                                                                                                 >  8:332682/1‑149 (MQ=255)
      gggTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGc                                                                                                                                              <  4:324970/149‑1 (MQ=255)
       ggTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCa                                                                                                                                             <  2:18721/149‑1 (MQ=255)
               tgCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAacca                                                                                                                                     <  2:214330/149‑1 (MQ=255)
                    gCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGt                                                                                                                                <  3:278455/149‑1 (MQ=255)
                                       gcAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCggg                                                                                                             >  2:319720/1‑149 (MQ=255)
                                       gcAGCCGTCGACCGGGCTGGCCGTGCCGTAAACCGCGATCACATACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGAGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCggg                                                                                                             <  6:337603/149‑1 (MQ=255)
                                         aGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTaa                                                                                                                                        <  6:36091/120‑1 (MQ=255)
                                         aGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTaa                                                                                                                                        >  5:36091/1‑120 (MQ=255)
                                             gTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGaaa                                                                                                       <  7:332682/149‑1 (MQ=255)
                                                gACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCa                                                                                                    >  4:72579/1‑149 (MQ=255)
                                                       cTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCgg                                                                                             >  4:358441/1‑149 (MQ=255)
                                                            cGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGa                                                                                        >  5:277902/1‑149 (MQ=255)
                                                                  ggAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCg                                                                                  >  5:230174/1‑149 (MQ=255)
                                                                   gAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACGGCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGCCGCCCg                                                                                  >  1:293903/1‑149 (MQ=255)
                                                                        cgcgATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGAcc                                                                            >  7:145867/1‑149 (MQ=255)
                                                                             tCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGtgtg                                                                       <  3:230913/149‑1 (MQ=255)
                                                                                 cTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTgtggtg                                                                    <  2:251011/148‑1 (MQ=255)
                                                                                 cTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTgtggtg                                                                    >  1:251011/1‑148 (MQ=255)
                                                                                 cTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGtggtgg                                                                   >  1:143531/1‑149 (MQ=255)
                                                                                    cacCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATCGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTggtggt                                                                  <  6:277902/147‑1 (MQ=255)
                                                                                      ccGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTcc                                                              >  6:163708/1‑149 (MQ=255)
                                                                                                gCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTc                                                               >  1:129932/1‑138 (MQ=255)
                                                                                                gCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTc                                                               <  2:129932/138‑1 (MQ=255)
                                                                                                 cAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGa                                                    >  2:30541/1‑148 (MQ=255)
                                                                                                      ggTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGa                                              <  3:358441/149‑1 (MQ=255)
                                                                                                       gtgtTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAg                                             >  6:213053/1‑149 (MQ=255)
                                                                                                       gtgtTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAg                                             <  3:268561/149‑1 (MQ=255)
                                                                                                                     ccAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAg                                             <  5:313250/135‑1 (MQ=255)
                                                                                                                     ccAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAg                                             >  6:313250/1‑135 (MQ=255)
                                                                                                                     ccAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCgg                               <  5:163708/149‑1 (MQ=255)
                                                                                                                               gcagcaTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCg                      >  7:208986/1‑149 (MQ=255)
                                                                                                                                gttacctgCACCCGGCTCACCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGt                     >  6:388595/8‑149 (MQ=255)
                                                                                                                                cagcaTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGt                     >  5:254847/1‑149 (MQ=255)
                                                                                                                                    cctgCACCCGGCTCACCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGgc                  <  5:388595/145‑1 (MQ=255)
                                                                                                                                     ctgctcccGGGTGTCCGGGGTGCAGGTAACCAGCGGTGAGGTCTTTCAGGGGGGTTTGGAAATCAGCTTCGGCCTGTCGCCCGGTGACCGTGTGGTTGTTTCCGGCGATCTGAAACTGa                                              <  4:367820/114‑1 (MQ=255)
                                                                                                                                     ctgCACCCGGCTCACCAGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCACCGCCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGa                                              >  3:367820/3‑119 (MQ=255)
                                                                                                                                            cGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAg         <  2:293903/149‑1 (MQ=255)
                                                                                                                                             gggTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAGc        <  8:208986/149‑1 (MQ=255)
                                                                                                                                                   gCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCGGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAGCCTGCAg  <  5:385913/149‑1 (MQ=255)
                                                                                                                                                   gCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAGCCTGCAg  <  6:230174/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAGCCGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGAC‑CCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAGCCTGCAG  >  NC_002947/2347476‑2347770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑