Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,352,147 C→T 8.7% G373D (GGC→GAC)  PP_2067 ← EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,352,1470CT 8.7% 129.4 / 2.9 47G373D (GGC→GAC) PP_2067EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base C (22/20);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

GGTCGCCACCAGTTGCGTCAACCCGGCCGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTGGCACTGGTCCAGCCCATCAGCCGGGGCA  >  NC_002947/2351999‑2352294
                                                                                                                                                    |                                                                                                                                                    
ggTCGCCACCAGTTGCGTCAACCCGGCCGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGc                                                                                                                                                      <  2:366914/149‑1 (MQ=255)
     ccaccaGTTGCGTCAACCCGGCCGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATa                                                                                                                                                 >  8:154058/1‑149 (MQ=255)
        ccaGTTGCGTCAACCCGGCCGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCa                                                                                                                                              <  5:193390/149‑1 (MQ=255)
              gCGTCAACCCGGCCGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGcac                                                                                                                                         >  1:59696/1‑148 (MQ=255)
                   aaCCCGGCCGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGt                                                                                                                                   >  2:50305/1‑149 (MQ=255)
                        ggCCGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACg                                                                                                                              <  2:138502/149‑1 (MQ=255)
                        ggCCGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACg                                                                                                                              <  2:59696/149‑1 (MQ=255)
                           cGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGga                                                                                                                           >  1:9422/1‑149 (MQ=255)
                                cGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGgatgat                                                                                                                       >  1:253186/1‑148 (MQ=255)
                                          aCCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCgg                                                                                                            >  4:4721/1‑149 (MQ=255)
                                                        aCATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTc                                                                                              <  5:113366/149‑1 (MQ=255)
                                                         cATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGTCAATACCACGCACCACCGTGGACGGgat                                                                                                                          <  8:47845/120‑1 (MQ=255)
                                                         cATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGTCAATACCACGCACCACCGTGGACGGgat                                                                                                                          >  7:47845/1‑120 (MQ=255)
                                                            cGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGc                                                                                          >  2:11753/1‑149 (MQ=255)
                                                             gagaACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGc                                                                                          >  6:116504/1‑148 (MQ=255)
                                                              agaACACCGCCGAGGCTGAGCCGGCTTCCCGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGc                                                                                          >  7:311966/1‑147 (MQ=255)
                                                              agaACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCa                                                                                        <  6:72352/149‑1 (MQ=255)
                                                                     cgccgAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGcccc                                                                                   >  3:360178/1‑147 (MQ=255)
                                                                     cgccgAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCcca                                                                                 <  4:360178/149‑1 (MQ=255)
                                                                     cgccgAGGCTGAGCCGGCATCACGCTTGTCCCGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCcca                                                                                 <  6:192091/149‑1 (MQ=255)
                                                                      gccgAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCTCTGTCGGCGCTCAGGCCAGCCCccag                                                                                <  1:11753/149‑1 (MQ=255)
                                                                            gCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAG‑CCCCAGCcagcag                                                                         <  2:9422/149‑1 (MQ=255)
                                                                               gAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGcc                                                                       >  2:322930/1‑149 (MQ=255)
                                                                                aGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCACCCCCCAGCCAGCAGcc                                                                       >  7:177864/1‑148 (MQ=255)
                                                                                 gCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCa                                                                     >  8:237126/1‑149 (MQ=255)
                                                                                  ccGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAg                                                                    >  6:274892/1‑149 (MQ=255)
                                                                                     gCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAgcgc                                                                 <  2:23389/149‑1 (MQ=255)
                                                                                     gCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAgcgc                                                                 <  2:17537/149‑1 (MQ=255)
                                                                                     gCTGCAGGCGTGTGCAGCCCGGCCACCGCCAGCACCGATATCGCAACCATGATGAATGGCTGGCCAATACCACGCACCTCCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAgcgc                                                                 <  8:177864/149‑1 (MQ=255)
                                                                                      cTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGcc                                                                <  7:154058/149‑1 (MQ=255)
                                                                                       ttCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCa                                                               >  5:158962/1‑149 (MQ=255)
                                                                                         cACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCa                                                               <  1:50305/147‑1 (MQ=255)
                                                                                                 tCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCaaaa                                                      <  3:4721/148‑1 (MQ=255)
                                                                                                           ccACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGTCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGc                                           >  5:111014/1‑148 (MQ=255)
                                                                                                             aCCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGTCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGAc                                        <  6:111014/149‑1 (MQ=255)
                                                                                                             aCCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCTAGCGCCATGATCAAAAA‑GCCGCTGGCGAc                                        >  1:171844/1‑149 (MQ=255)
                                                                                                               cGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCa                                      >  7:133225/1‑149 (MQ=255)
                                                                                                                gCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGAc                                        <  4:191352/146‑1 (MQ=255)
                                                                                                                gCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGAc                                        >  3:191352/1‑146 (MQ=255)
                                                                                                                       ccGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTg                              <  2:253186/149‑1 (MQ=255)
                                                                                                                          aTAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTGGCa                           >  6:131212/1‑149 (MQ=255)
                                                                                                                          aTAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTGGCa                           <  2:171844/149‑1 (MQ=255)
                                                                                                                                    cATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTGGCACTGGTCCAGc                 >  5:282018/1‑149 (MQ=255)
                                                                                                                                      tgatgaAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTGGCACTGGTCCAGccc               <  5:274892/149‑1 (MQ=255)
                                                                                                                                               gCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTGGCACTGGTCCAGCCCATCAGCCgg      <  5:131212/149‑1 (MQ=255)
                                                                                                                                                gtGGGCAATACCACGGACCAACGGGGACGGGATGTACACGTTGTCGGCACTTTAGGGGGTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATGAAAAATGCCGCTGGCGACCATGAACTTGGCACTGGTCCAGCCCATCAGCCgg      <  1:273689/148‑1 (MQ=255)
                                                                                                                                                 tGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTGGCACTGGTCCAGCCCATCAGCCgggg    >  7:126747/1‑149 (MQ=255)
                                                                                                                                                   gCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTGGCACTGGTCCAGCCCATCAGCCGGGGCa  >  5:23781/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTCGCCACCAGTTGCGTCAACCCGGCCGTACCGATGGCGCCACCCAGGTTGCGCAACATCGAGAACACCGCCGAGGCTGAGCCGGCTTCACGCTTGTCCAGCCCGGCCACCGCCAGCACCGATAACGCAACCATGATGAAAGGCTGGCCAATACCACGCACCACCGTGGACGGGATGATCACGTTGTCGGCACTGTCGGCGCTCAGGCCAGCCCCCAGCCAGCAGCCCAGCGCCATGATCAAAAA‑GCCGCTGGCGACCATGAACTTGGCACTGGTCCAGCCCATCAGCCGGGGCA  >  NC_002947/2351999‑2352294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑