Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,367,826 A→G 8.7% Q215R (CAA→CGA)  gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,367,8260AG 8.7% 128.0 / 2.5 46Q215R (CAA→CGA) gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (21/21);  new base G (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAATGCCGCCGAGCTGACAGTGCTGACCCGCGAGATCGAGCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTACGACGAGCAGTCGTTCCTCGGCCTGCCGCG  >  NC_002947/2367679‑2367973
                                                                                                                                                   |                                                                                                                                                   
ccAATGCCGCCGAGCTGACAGTGCTGACCCGCGAGATCGAGCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCa                                                                                                                                                     >  6:280322/1‑148 (MQ=255)
      ccgccgAGCTGACAGTGCTGACCCGCGAGATCGAGCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACga                                                                                                                                               <  7:59206/148‑1 (MQ=255)
       cgccgAGCTGACAGTGCTGACCCGCGAGATCGAGCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACgaga                                                                                                                                             <  4:32803/149‑1 (MQ=255)
                 aCAGTGCTGACCCGCGAGATCGAGCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAg                                                                                                                                    <  5:337110/148‑1 (MQ=255)
                     tGCTGACCCGCGAGATCGAGCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAg                                                                                                                               <  2:197986/149‑1 (MQ=255)
                            ccGCGAGATCGAGCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTcc                                                                                                                        >  8:279362/1‑149 (MQ=255)
                                  gaTCGAGCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAAt                                                                                                                  <  3:87823/149‑1 (MQ=255)
                                        gCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGgctgc                                                                                                            <  5:142651/149‑1 (MQ=255)
                                            gTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGa                                                                                                        <  1:183324/149‑1 (MQ=255)
                                             tGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGa                                                                                                        >  7:118436/1‑148 (MQ=255)
                                                  gCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCa                                                                                                  >  3:135932/1‑149 (MQ=255)
                                                  gCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCa                                                                                                  >  5:56318/1‑149 (MQ=255)
                                                      aGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTc                                                                                              <  7:83371/149‑1 (MQ=255)
                                                        gTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCAc                                                                                            <  5:280322/149‑1 (MQ=255)
                                                                        ccGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGGAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATgaa                                                                            <  6:87014/149‑1 (MQ=255)
                                                                          gACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATgaag                                                                           >  5:95618/1‑148 (MQ=255)
                                                                           aCTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATgaagaa                                                                         <  1:58490/149‑1 (MQ=255)
                                                                            cTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAAt                                                                        >  6:183152/1‑149 (MQ=255)
                                                                            cTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAAt                                                                        <  4:135932/149‑1 (MQ=255)
                                                                                 aaCCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCAcc                                                                   >  5:104848/1‑149 (MQ=255)
                                                                                    cGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCGCGCCCAAAACGAGAAACGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTc                                                                <  4:159169/149‑1 (MQ=255)
                                                                                    cGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGt                                                                 >  1:335852/1‑148 (MQ=255)
                                                                                    cGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTc                                                                <  7:279362/149‑1 (MQ=255)
                                                                                      aTGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCaa                                                              <  5:183152/149‑1 (MQ=255)
                                                                                       tGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCGAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAg                                                             >  3:99955/1‑149 (MQ=255)
                                                                                       tGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCGAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGAGAACCACTTCACCTTCCTGGGCTATGAAGAATTCGCCGGCAAg                                                             >  5:320260/1‑149 (MQ=255)
                                                                                         aaGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGCCAAggg                                                           >  5:69231/1‑149 (MQ=255)
                                                                                                          aGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATggcggc                                           >  3:178201/1‑148 (MQ=255)
                                                                                                              gTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTAt                                                                               >  4:139414/1‑108 (MQ=255)
                                                                                                              gTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTAt                                                                               <  3:139414/108‑1 (MQ=255)
                                                                                                              gTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGAt                                      >  6:158956/1‑149 (MQ=255)
                                                                                                                 gcgcAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGt                                   >  5:348218/1‑149 (MQ=255)
                                                                                                                       gTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATg                                                                              >  7:16572/1‑100 (MQ=255)
                                                                                                                       gTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATg                                                                              <  8:16572/100‑1 (MQ=255)
                                                                                                                       gTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTacga                             >  3:166627/1‑149 (MQ=255)
                                                                                                                        tGGAGCAAACCGCCTTTGGCCCCGCCCGAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCTATGGCGGCCAGATGGTCTacgac                            <  4:99955/149‑1 (MQ=255)
                                                                                                                        tGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTacgac                            >  4:10337/1‑149 (MQ=255)
                                                                                                                        ggtaGCACACCGGCTTTGGCCCCGCCCGAAACGACACAGGCGAAGTCAAGGCCTTCCTCGAATGTCTGGTGGATATCCACTTCACCTTCCTGGGCTTTGAAGAATTCACCGTCAAGGGCGTCGCCGATGGGGGCCAGATGGTCTacgac                            <  6:320260/146‑1 (MQ=255)
                                                                                                                           aGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTacgac                            >  2:296099/1‑146 (MQ=255)
                                                                                                                           aGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTAcgacga                          <  6:95618/148‑1 (MQ=255)
                                                                                                                            gCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTACGACGAg                         >  7:163787/1‑148 (MQ=255)
                                                                                                                                aCCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCAc                                                                    <  7:116261/101‑1 (MQ=255)
                                                                                                                                aCCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCAc                                                                    >  8:116261/1‑101 (MQ=255)
                                                                                                                                  cGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGTCGCCGATGGCGGCCAGATGGTCTACGACGAGCAGTCGt                  <  2:335852/149‑1 (MQ=255)
                                                                                                                                    ccTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTACGACGAGCAGTCGTTc                <  6:56318/149‑1 (MQ=255)
                                                                                                                                           ccccGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTACGACGAGCAGTCGTTCCTCGGcc         >  8:129466/1‑149 (MQ=255)
                                                                                                                                                  cAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTACGACGAGCAGTCGTTCCTCGGCCTGCcgcg  >  3:235159/1‑149 (MQ=255)
                                                                                                                                                   aaaaCGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTACGACGAGCAg                      <  7:97023/128‑1 (MQ=255)
                                                                                                                                                   aaaaCGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTACGACGAGCAg                      >  8:97023/1‑128 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCAATGCCGCCGAGCTGACAGTGCTGACCCGCGAGATCGAGCAGGTGCTGGCCGAGGTACGGGTGGTGGTGGCCGACTTCGAACCGATGAAGGCCAAGCTGCGTGAAGTGGTAGCGCAGGTGGAGCAAACCGCCTTTGGCCCCGCCCAAAACGAGAAAGGCGAAGTCAAGGCCTTCCTCGAATGGCTGCTGGATAACCACTTCACCTTCCTGGGCTATGAAGAATTCACCGTCAAGGGCGACGCCGATGGCGGCCAGATGGTCTACGACGAGCAGTCGTTCCTCGGCCTGCCGCG  >  NC_002947/2367679‑2367973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑