Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,378,650 G→C 7.1% V69L (GTT→CTT)  PP_2086 → CrfX family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,378,6500GC 7.1% 213.9 / 3.3 66V69L (GTT→CTT) PP_2086CrfX family protein
Reads supporting (aligned to +/- strand):  ref base G (25/36);  new base C (3/2);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATGACCGGGACGACCGGGACGACGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCC  >  NC_002947/2378502‑2378797
                                                                                                                                                    |                                                                                                                                                   
cAATGACCGGGACGACCGGGACGACGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATg                                                                                                                                                     <  8:5171/149‑1 (MQ=255)
      ccgggacgaccgggacgaCGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTgcgc                                                                                                                                               >  1:339889/1‑149 (MQ=255)
       cgggacgaccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTCGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGcc                                                                                                                                              >  4:184962/1‑149 (MQ=255)
         ggacgaccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGACCAATGGCTCGGCGCAACATGCGCCga                                                                                                                                            <  1:204052/149‑2 (MQ=255)
          gacgaccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGt                                                                                                                                           <  3:364687/149‑1 (MQ=255)
          gacgaccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGt                                                                                                                                           <  7:196905/149‑1 (MQ=255)
             gaccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCg                                                                                                                                        >  1:226486/1‑149 (MQ=255)
             gaccgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCAACATGCGCCgagcca                                                                                                                                        <  3:103145/149‑6 (MQ=255)
              accgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGt                                                                                                                                       >  6:127190/1‑149 (MQ=255)
              accgggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGt                                                                                                                                       <  4:365223/149‑1 (MQ=255)
                  ggacgacGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGac                                                                                                                                   <  2:226486/149‑1 (MQ=255)
                         gccgcTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTc                                                                                                                            >  6:20641/1‑149 (MQ=255)
                         gccgcTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTc                                                                                                                            <  8:175214/149‑1 (MQ=255)
                           cgcTTGCCTGGGTCGCGTGCTGAAAACCGCCACCCGCCAGGTTGGCGCGGGTGAGCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTccc                                                                                                                          <  8:96727/149‑1 (MQ=255)
                           cgcTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTccc                                                                                                                          <  7:25273/149‑1 (MQ=255)
                                 ccTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAAc                                                                                                                     <  4:144713/148‑1 (MQ=255)
                                    gggTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCCCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATCGCTCGGCGCATCTAGCGCCGGAGCGTCGACACTCTTCCCGCAACGCTg                                                                                                                 >  4:119357/1‑149 (MQ=255)
                                     gggcgcgTGCTTAAAACCCCCAACCGCCCGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGc                                                                                                                <  2:178840/146‑1 (MQ=255)
                                     ggTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGc                                                                                                                >  3:152868/1‑149 (MQ=255)
                                          cgTGCTGAAAACCGCCACCCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGG‑‑‑ATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGgca                                                                                                        <  4:162484/149‑1 (MQ=255)
                                           gTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATgg                                                                                                          <  2:339889/149‑1 (MQ=255)
                                           gTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATgg                                                                                                          <  4:370493/149‑1 (MQ=255)
                                           gTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATgg                                                                                                          <  4:152868/149‑1 (MQ=255)
                                            tGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCATTGgc                                                                                                         <  4:121800/149‑1 (MQ=255)
                                             gCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATgg                                                                                                          >  2:5053/1‑147 (MQ=255)
                                                 aaaaCCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGgcagca                                                                                                     >  6:66566/1‑148 (MQ=255)
                                                  aaaCCGCCAACGGCCTGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCATTGgcagcgt                                                                                                    <  4:175192/148‑3 (MQ=255)
                                                      cggcaaccgcgaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGGTGTTTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACActc                                                                                                                               <  2:244047/114‑1 (MQ=255)
                                                      cgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGt                                                                                                                                       >  2:263409/1‑109 (MQ=255)
                                                      cgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGt                                                                                                                                       <  1:263409/109‑1 (MQ=255)
                                                      cgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCAGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGAGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACActc                                                                                                                               >  1:244047/1‑117 (MQ=255)
                                                       gccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGa                                                                                              <  5:140342/149‑1 (MQ=255)
                                                          aaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGTCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGAt                                                                                             >  8:125567/1‑147 (MQ=255)
                                                               ccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGc                                                                                                                        >  6:232742/1‑115 (MQ=255)
                                                               ccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGc                                                                                                                        <  5:232742/115‑1 (MQ=255)
                                                                caGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGa                                                                                     <  5:20641/149‑1 (MQ=255)
                                                                   gTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGAtt                                                                                   >  5:290716/1‑148 (MQ=255)
                                                                    ttGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTg                                                                                  >  1:171679/1‑148 (MQ=255)
                                                                      ggCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGaa                                                                                <  6:37754/148‑1 (MQ=255)
                                                                       gcgcGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCt                                                                                                                  <  1:232990/113‑1 (MQ=255)
                                                                       gcgcGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCt                                                                                                                  >  2:232990/1‑113 (MQ=255)
                                                                          cgGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATgg                                                                           <  5:127190/149‑1 (MQ=255)
                                                                               gATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTc                                                                      >  3:336725/1‑149 (MQ=255)
                                                                               gATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTc                                                                      >  5:296928/1‑149 (MQ=255)
                                                                                  cTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAt                                                                   >  7:207136/1‑149 (MQ=255)
                                                                                    gTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGCCACTCTTCCCGCAACGCTGCCACTGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAtct                                                                 >  5:4595/1‑149 (MQ=255)
                                                                                     ttCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAtctc                                                                >  4:346507/1‑149 (MQ=255)
                                                                                         gCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGa                                                            <  2:171679/149‑1 (MQ=255)
                                                                                          ccTGCTTGGCCGCTGGAGTCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGAc                                                           <  7:125567/149‑1 (MQ=255)
                                                                                               ttGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGACCTCGCTCTCTGGACCCa                                                        >  2:284114/1‑147 (MQ=255)
                                                                                                  gCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAt                                                                   >  4:184919/1‑133 (MQ=255)
                                                                                                  gCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAt                                                                   <  3:184919/133‑1 (MQ=255)
                                                                                                         gAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTgggc                                              <  8:207136/147‑3 (MQ=255)
                                                                                                                       tgATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCt                                                  <  4:219809/129‑1 (MQ=255)
                                                                                                                       tgATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCt                                                  >  3:219809/1‑129 (MQ=255)
                                                                                                                            gCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTg                          >  2:136546/1‑148 (MQ=255)
                                                                                                                            gCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTg                          <  6:296928/148‑1 (MQ=255)
                                                                                                                            gCCGGGAACAATGGCTCGGCGCCTGTTGCGCCGGTGCGGCGACACACTTCCCGCAACGCTTCCAATTGCAGCAAACCACATAATGCCGCATGAATATGGAACTCGATCTCTGGACCCACCTCCTGGTCACCGCGATGACTGCCCTTTg                          <  2:358410/148‑1 (MQ=255)
                                                                                                                             ccGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGCCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTgg                         <  4:336725/148‑1 (MQ=255)
                                                                                                                                tGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCa                     <  5:66566/149‑1 (MQ=255)
                                                                                                                                       tGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGc              >  1:375918/1‑149 (MQ=255)
                                                                                                                                         caccGGCGCAACATGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCg             >  7:206002/4‑148 (MQ=255)
                                                                                                                                          accGGCGCACCATGCGCCGGTGCGTCGACACTCTGCCCGCAACGCTGCCAATGGCAGCAAAGCAGAGAAGGCCGATTGAAGATGGAACTCGATCTCTGGACCCAGAGCCTGGTCCCCGCGATGACTGCCCTTTGGACCAAGGTAGCGaa           >  1:58041/3‑149 (MQ=255)
                                                                                                                                              gcggATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTc       <  3:184962/149‑1 (MQ=255)
                                                                                                                                              gcgcATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCTGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTc       <  1:284114/149‑1 (MQ=255)
                                                                                                                                                gcATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCAt     <  1:136546/149‑1 (MQ=255)
                                                                                                                                                 cATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATc    <  1:5053/149‑1 (MQ=255)
                                                                                                                                                    gTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATccc  >  6:135422/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAATGACCGGGACGACCGGGACGACGCCGCTTGCCTGGGTCGCGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCC  >  NC_002947/2378502‑2378797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑