Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,442,668	G→T	9.0%	P602P (CCG→CCT)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,442,668	0	G	T	9.0%	219.5 / 8.3	74	P602P (CCG→CCT)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base G (29/37);  new base T (3/4);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAGAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGC  >  NC_002947/2442520‑2442805                                                                                                                                                     |                                                                                                                                           aaGAGAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCg                                                                                                                                            >  6:121497/1‑149 (MQ=255)      aaCCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGc                                                                                                                                         >  4:133884/1‑147 (MQ=255)      aaCCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCaa                                                                                                                                       <  8:111934/149‑1 (MQ=255)         cTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAgg                                                                                                                                     >  4:298403/1‑148 (MQ=255)           cgacaaCGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAAt                                                                                                                                  >  8:305607/1‑149 (MQ=255)              cgacgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACC‑ACATTCCTTGCACGGAAtg‑ttg                                                                                                                             <  7:14521/149‑3 (MQ=255)               gacgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCTTGCACGGAAtg‑ttg                                                                                                                             <  7:115327/149‑3 (MQ=255)               gacgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑Tgg                                                                                                                             <  8:299247/149‑1 (MQ=255)                 cgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCTTGCACGGAAtg‑ttggt                                                                                                                           <  2:176463/149‑5 (MQ=255)                   tCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCgg                                                                                                                         <  4:792/149‑1 (MQ=255)                       ccAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAAtg‑tg                                                                                                                              >  2:301002/1‑140 (MQ=255)                       ccAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAAtg‑tg                                                                                                                              <  1:301002/140‑1 (MQ=255)                       ccAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCga                                                                                                                     >  8:268119/1‑149 (MQ=255)                          gggCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatga                                                                                                                  <  1:374263/149‑1 (MQ=255)                            gCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatgatg                                                                                                                <  2:157374/149‑1 (MQ=255)                             cGAGCGTGACTGGAAGAACGTGCGCGACGAGTTCTACGGCGTATTCAGCAAAATGCTGCAGTCTGGCGAGTCCAGCGAAGACGGCATGCTTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatgatga                                                                                                               <  6:112094/149‑1 (MQ=255)                               aGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCTTGCAAGGAATG‑TGGTCGGCCGATGATGAtt                                                                                                             <  1:225541/149‑1 (MQ=255)                               aGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGAtt                                                                                                             <  3:298403/149‑1 (MQ=255)                                gCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTc                                                                                                            <  3:133884/149‑1 (MQ=255)                                   tGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGAtt                                                                                                             <  5:58100/145‑1 (MQ=255)                                   tGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGAtt                                                                                                             >  6:58100/1‑145 (MQ=255)                                     aCTGGAAGAACGTGCTCGACGAGTTCTAGGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAc                                                                                                        <  8:353758/148‑1 (MQ=255)                                     aCTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGGCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACGAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAcc                                                                                                       <  5:121497/149‑1 (MQ=255)                                        ggAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCgg                                                                                                                         <  8:296278/128‑1 (MQ=255)                                        ggAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCgg                                                                                                                         >  7:296278/1‑128 (MQ=255)                                         gaagaaCGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCt                                                                                                    >  1:357071/1‑148 (MQ=255)                                         gaagaaCGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCAGATGATGCTTCGCACCGCtt                                                                                                   >  2:186499/1‑149 (MQ=255)                                            gaaCGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTa                                                                                                >  3:57793/1‑149 (MQ=255)                                               cGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACgg                                                                                             <  4:57793/149‑1 (MQ=255)                                               cGTGCTCGACGAGTTATACCGCGATTTCAGCAAAAACCTGCAGACTACCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCCTGCAAGGAATG‑TGGTCGCCCGATGATGATTCGCACAGCTTCTACgg                                                                                             >  8:114918/1‑149 (MQ=255)                                                  gCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                    <  3:282120/123‑1 (MQ=255)                                                  gCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                    >  4:282120/1‑123 (MQ=255)                                                  gCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgtg                                                                                          >  4:87784/1‑149 (MQ=255)                                                      gacgaGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgt                                                                                           >  2:353547/1‑144 (MQ=255)                                                      gacgaGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgt                                                                                           <  1:353547/144‑1 (MQ=255)                                                       acgaGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTc                                                                                     >  5:266742/1‑149 (MQ=255)                                                         gaGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGc                                                                                                     <  7:76441/131‑1 (MQ=255)                                                         gaGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGc                                                                                                     >  8:76441/1‑131 (MQ=255)                                                               tACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCt                                                                                   <  5:183392/143‑1 (MQ=255)                                                               tACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCt                                                                                   >  6:183392/1‑143 (MQ=255)                                                                   gCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                         <  6:86324/149‑1 (MQ=255)                                                                     gATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTgg                                                                       <  3:87784/149‑1 (MQ=255)                                                                             cAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTgg                                                                       >  4:38275/1‑141 (MQ=255)                                                                             cAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTgg                                                                       <  3:38275/141‑1 (MQ=255)                                                                                       cAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                         >  6:85405/1‑129 (MQ=255)                                                                                       cAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                         <  5:85405/129‑1 (MQ=255)                                                                                              ccGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgt                                                                                           <  4:127246/104‑1 (MQ=255)                                                                                              ccGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgt                                                                                           >  3:127246/1‑104 (MQ=255)                                                                                              ccGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTg                                                                             >  6:159992/1‑118 (MQ=255)                                                                                              ccGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTg                                                                             <  5:159992/118‑1 (MQ=255)                                                                                                   tCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCaa                                         <  2:357071/149‑1 (MQ=255)                                                                                                     cAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAgg                                       <  6:266742/149‑1 (MQ=255)                                                                                                     cAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAgg                                       >  2:359118/1‑149 (MQ=255)                                                                                                       gCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGc                                      >  8:273129/1‑148 (MQ=255)                                                                                                           aGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCg                                 <  7:273129/149‑1 (MQ=255)                                                                                                             aCGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCt                                     <  2:80588/143‑1 (MQ=255)                                                                                                             aCGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCt                                     >  1:80588/1‑143 (MQ=255)                                                                                                                 cATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatgat                                                                                                                 >  5:76019/1‑63 (MQ=255)                                                                                                                 cATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatgat                                                                                                                 <  6:76019/63‑1 (MQ=255)                                                                                                                         ccAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGcc                    <  5:333909/148‑1 (MQ=255)                                                                                                                         ccAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCGGCAAGGCTACCGTCAACCTGGTGCCg                   >  3:54905/1‑149 (MQ=255)                                                                                                                           aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                         >  7:89144/1‑93 (MQ=255)                                                                                                                           aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                         <  8:89144/93‑1 (MQ=255)                                                                                                                            aCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGc                >  1:186301/1‑149 (MQ=255)                                                                                                                               aGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTCCAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGcgac             <  7:305607/149‑1 (MQ=255)                                                                                                                                gCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTg                                                                        <  6:45067/89‑1 (MQ=255)                                                                                                                                 ccGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGcgacga           <  7:268119/149‑1 (MQ=255)                                                                                                                                   gagcATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCGTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGTCgaga         <  7:114918/146‑1 (MQ=255)                                                                                                                                          gaccaCATTCCTTGCACGGAATGTTGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCg   >  2:157665/5‑149 (MQ=255)                                                                                                                                           accaCATTCCTTGCACGGAATGTTGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGc  >  5:107811/4‑149 (MQ=255)                                                                                                                                           accaCATTCCTTGCACGGAATGTTGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGc  >  4:276545/4‑149 (MQ=255)                                                                                                                                                 ttCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTAc                                   <  4:88307/109‑1 (MQ=255)                                                                                                                                                 ttCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTAc                                   >  3:88307/1‑109 (MQ=255)                                                                                                                                                 ttCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGt                                >  2:218647/1‑112 (MQ=255)                                                                                                                                                 ttCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGt                                <  1:218647/112‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                           AAGAGAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGC  >  NC_002947/2442520‑2442805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑