| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,469,844 | G→A | 13.0% | intergenic (+13/+22) | vacJ → / ← PP_2164 | lipoprotein VacJ/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,469,844 | 0 | G | A | 13.0% | 217.5 / 17.0 | 84 | intergenic (+13/+22) | vacJ/PP_2164 | lipoprotein VacJ/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (40/33); new base A (6/5); total (46/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CCTGATCGACACCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTAC > NC_002947/2469696‑2469988 | ccTGATCGACACCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTgg > 7:130231/1‑149 (MQ=255) gATCGACACCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATGCGCAATGCCTACCTGCAAAACCGCGAGTTCGAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGc > 5:272913/1‑149 (MQ=255) acacCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGctt > 8:222665/1‑147 (MQ=255) acCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAg > 8:254919/1‑149 (MQ=255) ccGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGctttgcg > 5:302043/1‑143 (MQ=255) cgcgCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCgg < 8:244021/149‑1 (MQ=255) cgcgCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCgg < 6:353583/149‑1 (MQ=255) gcCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCt > 8:11892/1‑149 (MQ=255) ccTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGCTTTGCGGCCCCAGGt < 6:302043/149‑1 (MQ=255) ctctCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCaa < 7:254919/149‑1 (MQ=255) ctctCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGCTTTGCGGCCCCAGGTTCac < 7:222665/149‑2 (MQ=255) tctcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGCTTTGCGGCCCCAGGTTCa > 3:116234/1‑147 (MQ=255) tcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATg > 1:281731/1‑148 (MQ=255) tcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGCTTTGCGGCCCCAGGTTCaca < 4:116234/147‑3 (MQ=38) ggCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCAt > 1:128178/1‑149 (MQ=255) gCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGCTTTGCGGccccag < 7:87400/137‑5 (MQ=255) gCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGCTTTGCGGccccag > 8:87400/1‑133 (MQ=255) gAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGcc < 2:333564/149‑1 (MQ=255) aaaGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGc > 4:167661/1‑146 (MQ=255) aGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGaa > 4:51742/1‑149 (MQ=255) gCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAAt < 3:167661/149‑1 (MQ=255) cTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATc < 7:11892/149‑1 (MQ=255) gATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCg > 5:259555/1‑149 (MQ=255) ttCATGGTGCCAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCa < 4:255545/149‑1 (MQ=255) aGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCGAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGc < 6:272913/148‑1 (MQ=255) aGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGc < 2:48811/148‑1 (MQ=255) aCAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTCAAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGaa < 1:298813/148‑1 (MQ=255) cAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACt > 2:98160/1‑148 (MQ=255) aaGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTc > 2:17196/1‑149 (MQ=255) aaGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTc < 2:128178/149‑1 (MQ=255) gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGtt > 2:169016/1‑114 (MQ=255) gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctg < 7:102438/149‑1 (MQ=255) aTCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTTAATGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCTGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCtgctgc < 2:74826/149‑1 (MQ=255) tCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgcc > 5:8777/1‑149 (MQ=255) cTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgccc > 5:221198/1‑149 (MQ=255) cTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgccc > 2:353645/1‑149 (MQ=255) aTTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGCTTTGCGGCCCCAGGtt > 5:256077/1‑104 (MQ=37) aTTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCCGAGGCCGCTTTGCGGCCCCAGGtt < 6:256077/104‑1 (MQ=37) ttCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGccc < 4:262275/129‑1 (MQ=255) ttCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGccc > 3:262275/1‑129 (MQ=255) gCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGc < 1:98160/148‑1 (MQ=255) ccTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCgg > 2:44625/1‑149 (MQ=255) cTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCAt > 8:283816/1‑101 (MQ=255) cTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCAt < 7:283816/101‑1 (MQ=255) cTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATc > 1:309297/1‑149 (MQ=255) gCAAAACCGCGAGTTAAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGTTACAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCAGGCTGGTCGGCAATcc > 5:229482/1‑148 (MQ=255) aaaaCCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATa < 2:281731/149‑1 (MQ=255) cgcgAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACt > 2:284367/1‑149 (MQ=255) cgcgAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACt > 3:224174/1‑149 (MQ=255) gAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGCTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCg > 5:323330/1‑149 (MQ=255) aGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCgg < 8:130231/149‑1 (MQ=255) gTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGt > 3:183272/1‑149 (MQ=255) tCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCCGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGc > 2:4187/1‑149 (MQ=255) cAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCt > 3:270293/1‑149 (MQ=255) gTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCg < 5:274312/122‑1 (MQ=255) gTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCg > 6:274312/1‑122 (MQ=255) gTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCa > 6:315626/1‑149 (MQ=255) ggATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGcc < 2:178722/88‑1 (MQ=255) ggATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGcc > 1:178722/1‑88 (MQ=255) gCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcccc < 3:51742/148‑1 (MQ=255) cGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATAc > 8:318892/1‑122 (MQ=255) cGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATAc < 7:318892/122‑1 (MQ=255) gAGGTCGAAGACGACTTCTGATCCGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTc < 1:4187/149‑1 (MQ=255) aGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCa < 6:221198/149‑1 (MQ=255) aGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCa < 2:309297/149‑1 (MQ=255) aGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCa < 4:183272/149‑1 (MQ=255) gTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTgg > 5:69729/1‑132 (MQ=255) gTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTgg < 6:69729/132‑1 (MQ=255) gTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAgc > 2:90412/1‑149 (MQ=255) gTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAgc > 2:349250/1‑149 (MQ=255) gAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTg < 5:200489/128‑1 (MQ=255) gAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTg > 6:200489/1‑128 (MQ=255) gAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCtt > 4:61584/1‑142 (MQ=255) gAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCtt < 3:61584/142‑1 (MQ=255) aaGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAACGCGGCCGCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATCCCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCg < 6:323330/149‑1 (MQ=255) gacgacTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctgc < 4:270717/83‑1 (MQ=255) gacgacTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctgc > 3:270717/1‑83 (MQ=255) gacTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCt > 4:305526/1‑121 (MQ=255) cttaTCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCTGCAATCCATACCACTTCGGTGTTGGCATGCAGCCCCTTCAGCGCCGGATGGACgg < 1:44625/145‑1 (MQ=255) cTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACg > 2:238073/1‑147 (MQ=255) aTCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGt < 1:238073/148‑1 (MQ=255) cTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGa > 5:288921/1‑149 (MQ=255) att‑gAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGAt > 6:20949/3‑149 (MQ=38) gt‑gAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATc > 5:55306/1‑149 (MQ=255) ccTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTAc > 7:46191/1‑149 (MQ=255) | CCTGATCGACACCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGT‑GAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTAC > NC_002947/2469696‑2469988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |