Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,579,758 T→C 7.8% L25P (CTG→CCG)  PP_2261 → sugar ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,579,7580TC 7.8% 146.1 / 2.4 51L25P (CTG→CCG) PP_2261sugar ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

CAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGACGGCAAGCCGGTC  >  NC_002947/2579613‑2579903
                                                                                                                                                  |                                                                                                                                                 
caAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTgca                                                                                                                                                >  5:202544/1‑149 (MQ=255)
 aaGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGCAGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCACCGAGGCAGACAATGCCCTgca                                                                                                                                                >  1:108834/1‑149 (MQ=255)
           ttttCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGaa                                                                                                                                      >  6:36691/1‑148 (MQ=255)
                gACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACAtgtg                                                                                                                                >  2:372602/1‑149 (MQ=255)
                gACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGCAGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACAtgtg                                                                                                                                <  7:203970/149‑1 (MQ=255)
                   aGCGCCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTgg                                                                                                                             <  5:36691/149‑1 (MQ=255)
                    gcgcCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTggg                                                                                                                            <  3:123331/149‑1 (MQ=255)
                      gcCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGaa                                                                                                                          <  2:108834/149‑1 (MQ=255)
                      gcCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCCGCACGCACTCGAACATGTGTGGGaa                                                                                                                          >  2:341471/1‑149 (MQ=255)
                             tGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCg                                                                                                                   >  1:183511/1‑149 (MQ=255)
                             tGTTGGACCAGGCCCCGCGCCTGCGCCACGC‑GACGACAGGGTGATGGCCGAAATCCGCCTGCGCCAGCAGCCGCACACTAACAGCCGCCACCCAGCCACCGAGGCAGACTATGCCCTCCACGCACTCGAACAAGAGAGGCAACacgccg                                                                                                                   >  4:40388/1‑144 (MQ=255)
                               ttGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGACCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGt                                                                                                                 >  2:165547/1‑149 (MQ=255)
                                  gTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGc                                                                                                               <  4:301176/148‑1 (MQ=255)
                                   tGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCt                                                                                                             <  2:183511/149‑1 (MQ=255)
                                    gCAGCCCCGGGGCCTGCACCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTa                                                                                                            >  7:174843/1‑149 (MQ=255)
                                     cAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTa                                                                                                            <  6:296794/148‑1 (MQ=255)
                                     cAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTa                                                                                                            <  6:95060/148‑1 (MQ=255)
                                        gCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCg                                                                                                        <  1:372602/149‑1 (MQ=255)
                                         ccccGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGt                                                                                                       <  3:144685/149‑1 (MQ=255)
                                          cccGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGtt                                                                                                      <  1:314853/149‑1 (MQ=255)
                                              gggCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTc                                                                                                  >  7:145360/1‑149 (MQ=255)
                                                gCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCgg                                                                                                <  3:40388/149‑1 (MQ=255)
                                                 cTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAGATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGGGTTGCTCggg                                                                                               <  2:318730/149‑1 (MQ=255)
                                                    cgcCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGc                                                                                              <  7:127275/147‑1 (MQ=255)
                                                     gcCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGt                                                                                           >  6:53757/1‑149 (MQ=255)
                                                       cAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCg                                                                                         >  1:379580/1‑149 (MQ=255)
                                                          gc‑gcGGAGCGGGGGATGGCCGAACTCCGCCTGCGCCAGCTGGCGCACACTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTCTGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGc                                                                                      <  2:340297/149‑1 (MQ=255)
                                                                 agagGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCCGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGc                                                                                <  1:341471/149‑1 (MQ=255)
                                                                   agGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCaa                                                                              <  6:202544/149‑1 (MQ=255)
                                                                          tGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGAcc                                                                       <  2:379580/149‑1 (MQ=255)
                                                                           ggCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCCGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCt                                                                      >  8:155170/1‑149 (MQ=255)
                                                                                            gcCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTcc                                                     >  4:204832/1‑149 (MQ=255)
                                                                                               aGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCggg                                                  >  2:89013/1‑149 (MQ=255)
                                                                                                  tGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTg                                                                    >  8:276006/1‑128 (MQ=255)
                                                                                                    gcgcACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGgctgct                                             <  5:53757/149‑1 (MQ=255)
                                                                                                            ttACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGAc                                                                        <  2:184916/114‑1 (MQ=255)
                                                                                                            ttACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGAc                                                                        >  1:184916/1‑114 (MQ=255)
                                                                                                                     ccagcccgccagcGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCggg                                                                                       <  7:219380/90‑1 (MQ=255)
                                                                                                                     ccagcccgccagcGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCggg                                                                                       >  8:219380/1‑90 (MQ=255)
                                                                                                                       agcccgccagcGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAg                          <  3:204832/149‑1 (MQ=255)
                                                                                                                         cccgccagcGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGt                        >  6:258587/1‑149 (MQ=255)
                                                                                                                              cagcGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGt                   >  1:339732/1‑149 (MQ=255)
                                                                                                                                   aGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGAc              <  8:145360/149‑1 (MQ=255)
                                                                                                                                     gCAGACTATGCCCCGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGACgg            <  7:155170/149‑1 (MQ=255)
                                                                                                                                       aGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGACGGc           >  5:270913/1‑148 (MQ=255)
                                                                                                                                         aCTATGCCCTGCACGCACTCGAACATGTGGGGGAACAGGTCGGTGCCTATGCGGTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATTTCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGACGGCAAg        <  1:266392/149‑1 (MQ=255)
                                                                                                                                          cTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGACGGCAAGc       >  1:22343/1‑149 (MQ=255)
                                                                                                                                               cccTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGACGGCAAGCCGGt   >  5:13315/1‑148 (MQ=255)
                                                                                                                                               cccTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGACGGCAAGCCGGTc  <  6:270913/149‑1 (MQ=255)
                                                                                                                                                ccTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGACGGCAAGCCGGTc  >  3:53622/1‑148 (MQ=255)
                                                                                                                                                 cTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTAGGGCCGTCGGGCTGCGGCAAGTCGACATTGCTAAATATCATTTCCGGGCTGCTGACGCCGTCTCAtt                               >  4:219477/1‑117 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGCTGCGCCAGGC‑GAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGCCTATGCGTTGCTCGGGCCGTCGGGCTGCGGCAAGTCGACCTTGCTCAATATCATTTCCGGGCTGCTGACGCCGTCTCATGGCGAGGTGCTGTTCGACGGCAAGCCGGTC  >  NC_002947/2579613‑2579903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑