Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,621,328	T→A	7.6%	L48H (CTT→CAT)	PP_2294 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,621,328	0	T	A	7.6%	193.6 / 4.9	66	L48H (CTT→CAT)	PP_2294	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base A (2/3);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

TGTCGAGACGGGCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGATTCAGACAACGTATCGCTCAACCTAGACGGGAA  >  NC_002947/2621190‑2621475                                                                                                                                           |                                                                                                                                                     tgTCGAGACGGGCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAg                                                                                                                                            >  4:209480/1‑149 (MQ=255)  gTCGAGACGGGCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAgg                                                                                                                                           >  5:56126/1‑149 (MQ=255)   tCGAGACGGGCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGa                                                                                                                                                   >  4:301972/1‑140 (MQ=255)   tCGAGACGGGCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGa                                                                                                                                                   <  3:301972/140‑1 (MQ=255)     gagaCGGGCGATTGGGATGGTGTTGGCCCTTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCATCAAGCTGAgcata                                                                                                                                        <  4:14706/149‑5 (MQ=255)      agaCGGGCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTg‑g                                                                                                                                      >  8:249706/1‑149 (MQ=255)      agaCGGGCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCATCAAGCTGAgcatag                                                                                                                                       <  1:275703/149‑6 (MQ=255)        aCGGGCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCt                                                                                                                                    <  4:147156/149‑1 (MQ=255)          gggCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTACCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTg                                                                                                                                   >  1:183094/1‑148 (MQ=255)            gCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCaa                                                                                                                                <  4:223327/149‑1 (MQ=255)                ttGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGc                                                                                                                            <  3:209480/149‑1 (MQ=255)                     aTCGTGTTGGCCATTGGAGTTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATa                                                                                                                       <  4:326888/149‑1 (MQ=255)                     aTCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATa                                                                                                                       <  2:312276/149‑1 (MQ=255)                           ttGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTg                                                                                                                  <  6:56126/148‑1 (MQ=255)                           ttGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGa                                                                                                                 >  7:118912/1‑149 (MQ=255)                                cATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGAt                                                                                                                <  6:158120/145‑1 (MQ=255)                                cATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGAt                                                                                                                >  5:158120/1‑145 (MQ=255)                                 aTTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCagaa                                                                                                           <  8:118912/149‑1 (MQ=255)                                           ctTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCAGAAAGCGCAGAGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGa                                                                                                  >  7:32992/1‑148 (MQ=255)                                            ttATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATAAGAAGAAGGATGACa                                                                                                >  5:390136/1‑149 (MQ=255)                                               tgttgtCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACAttt                                                                                             >  6:88022/1‑149 (MQ=255)                                                   gtCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAag                                                                                                                               >  4:375739/1‑111 (MQ=255)                                                   gtCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAag                                                                                                                               <  3:375739/111‑1 (MQ=255)                                                     ccTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGtt                                                                                                                                         <  7:255703/100‑1 (MQ=255)                                                     ccTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGtt                                                                                                                                         >  8:255703/1‑100 (MQ=255)                                                      cTCAAAGGAGTAGATAACGGGCCTAGCTCTTCCTCTTCAAGAGATCCAGAGGTTTATCCCGAAAGCGCAGTGACCAATGCTAAGCTTGAGGCTGAGGTTG‑GATGAAAGATAATATACCGTGATCAGAAAAAGGATCACATTTTGGATTc                                                                                      >  2:166813/1‑149 (MQ=255)                                                       tCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTcc                                                                                     >  3:187635/1‑149 (MQ=255)                                                       tCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTcc                                                                                     >  2:94911/1‑149 (MQ=255)                                                         aaaGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATACCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCctc                                                                                   >  1:369404/1‑149 (MQ=255)                                                           aGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGAt                                                                                                    <  2:271397/130‑1 (MQ=255)                                                           aGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGAt                                                                                                    >  1:271397/1‑130 (MQ=255)                                                                   cTAACGCGCCTAGCTCTTCCTCTTCAAATGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACaaaaa                                                                          >  1:208691/1‑148 (MQ=255)                                                                     aaCGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATc                                                                        >  6:345234/1‑148 (MQ=255)                                                                      aCGCGCCTAGCTCTTCCTCTTCAATTTCTCCAGAGGTTTATGCCGAAAGAGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCAc                                                                      <  6:329854/149‑1 (MQ=255)                                                                      aCGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTGACCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAAt                                                                         <  1:298532/147‑1 (MQ=255)                                                                               gCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGAcc                                                             <  5:345234/149‑1 (MQ=255)                                                                                 tcttcctcttcAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTcc                                                                                     >  6:383608/1‑123 (MQ=255)                                                                                 tcttcctcttcAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTcc                                                                                     <  5:383608/123‑1 (MQ=255)                                                                                   ttcctcttcAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCt                                                                                                                                    >  2:148626/1‑74 (MQ=255)                                                                                   ttcctcttcAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCt                                                                                                                                    <  1:148626/74‑1 (MQ=255)                                                                                        cttcAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCAt                                                           <  5:99747/142‑1 (MQ=255)                                                                                        cttcAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCAt                                                           >  6:99747/1‑142 (MQ=255)                                                                                          tcAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCt                                                   >  4:301230/1‑148 (MQ=255)                                                                                                gCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTg                                             <  1:94911/148‑1 (MQ=255)                                                                                                 cTCCAGAGTTTTATCCCTAAAGCGCAGTGACCTATTCTCAGATTTATGATTAGGTTG‑GCTGCAATAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGAc                                           <  1:271842/149‑1 (MQ=255)                                                                                                    cAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCtt                                        >  2:63515/1‑149 (MQ=255)                                                                                                      gagGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTtgt                                      <  3:301230/149‑1 (MQ=255)                                                                                                      gagGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTtgt                                      <  5:88022/149‑1 (MQ=255)                                                                                                          tttATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGAt                                  <  4:176295/149‑1 (MQ=255)                                                                                                            tATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGCAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGAtt                                 >  8:267369/1‑148 (MQ=255)                                                                                                                ccGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCt                                                   <  6:358500/126‑1 (MQ=255)                                                                                                                ccGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCt                                                   >  5:358500/1‑126 (MQ=255)                                                                                                                 cGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGAcc                                                             <  7:265334/115‑1 (MQ=255)                                                                                                                 cGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGAcc                                                             >  8:265334/1‑115 (MQ=255)                                                                                                                  gAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGGAAGATCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGATTCAGACaa                          <  2:183094/149‑1 (MQ=255)                                                                                                                        gcAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGATTCAGACAACGTATc                    >  6:314470/1‑149 (MQ=255)                                                                                                                                     cTCAGCATCAAGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGa                                   >  6:129064/1‑121 (MQ=255)                                                                                                                                     cTCAGCATCAAGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGa                                   <  5:129064/121‑1 (MQ=255)                                                                                                                                     cTCAGCATCAAGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGATTCAGACAACGTATCGCTCAACCTAGAc       >  1:111458/1‑149 (MQ=255)                                                                                                                                      tCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGATGAAGGATGACATTTTCGATTCCTCTTTCAAAAATCACTGGATGACCATGAAAGGCTAAATTGACCTTGTGGATTCATCCAACGTATAGCTCAACCTAGACg      >  7:110871/1‑149 (MQ=255)                                                                                                                                      tCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTtgt                                      >  6:376119/1‑117 (MQ=255)                                                                                                                                      tCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTtgt                                      <  5:376119/117‑1 (MQ=255)                                                                                                                                        aGCTTGATGCTTAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGATTAATGATTACATTTTCGATTCCTCTTACAAAAATCAATGGATGACCATGAAAGGCTCAATTGACCTTGTGGATTCAGACAACGTATCGCTCAACCTAGACgg     <  7:158411/148‑1 (MQ=255)                                                                                                                                         gCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTtgt                                      >  2:23845/1‑114 (MQ=255)                                                                                                                                         gCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTtgt                                      <  1:23845/114‑1 (MQ=255)                                                                                                                                          cTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGATTCAGACAACGTATCGCTCAACCTAGACGGGaa  <  7:249706/149‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                     TGTCGAGACGGGCGATTGGGATCGTGTTGGCCATTGGAGCTGCTTATGTTGTCCTCAAAGGAGTAGCTAACGCGCCTAGCTCTTCCTCTTCAAGTGCTCCAGAGGTTTATCCCGAAAGCGCAGTGACCTATGCTCAGCTTGATGCTGAGGTTG‑GCTGCAAGAGCAAATACAGTGATCAGAAGAAGGATGACATTTTCGATTCCTCTTACAAAAATCACTGGATGACCATGAAAGGCTCAATTGACCTTGTGGATTCAGACAACGTATCGCTCAACCTAGACGGGAA  >  NC_002947/2621190‑2621475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑