Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,628,189	A→T	6.9%	K425M (AAG→ATG) ‡	tig →	trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,628,189	0	A	T	6.9%	261.6 / 7.5	87	K425M (AAG→ATG) ‡	tig	trigger factor
Reads supporting (aligned to +/- strand):  ref base A (43/38);  new base T (4/2);  total (47/40)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

CGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAG  >  NC_002947/2628043‑2628335                                                                                                                                                   |                                                                                                                                                   cGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGCAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGt                                                                                                                                                  <  2:174266/149‑1 (MQ=255)  gaagaaATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGt                                                                                                                                                  <  2:306525/148‑1 (MQ=255)            cTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTAc                                                                                                                                       >  1:54849/1‑149 (MQ=255)             ttCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGtctc                                                                                                                                           >  2:256848/1‑144 (MQ=255)             ttCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGtctc                                                                                                                                           <  1:256848/144‑1 (MQ=255)               cGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACTTGTCGGtcacagtcg                                                                                                                                      <  6:139417/147‑8 (MQ=255)                ggCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACgaag                                                                                                                                   <  1:355200/149‑1 (MQ=255)                   ttACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGaagaag                                                                                                                                >  1:244245/1‑149 (MQ=255)                     aCCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGcc                                                                                                                              >  4:560/1‑149 (MQ=255)                      ccAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCg                                                                                                                             >  7:115727/1‑149 (MQ=255)                       cAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGt                                                                                                                            >  5:282899/1‑149 (MQ=255)                        aGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGtt                                                                                                                           <  2:54849/149‑1 (MQ=255)                        aGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGt                                                                                                                            >  1:174918/1‑148 (MQ=255)                          gAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTaa                                                                                                                         <  3:144266/149‑1 (MQ=255)                            gCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAAc                                                                                                                       <  3:221802/149‑1 (MQ=255)                                gAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGc                                                                                                                   >  3:365152/1‑149 (MQ=255)                                  gCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGcc                                                                                                                  >  7:121557/1‑148 (MQ=255)                                      gTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGt                                                                                                                                              >  6:281277/1‑116 (MQ=255)                                      gTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGt                                                                                                                                              <  5:281277/116‑1 (MQ=255)                                      gTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGgc                                                                                                             >  8:291019/1‑149 (MQ=255)                                       tCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGgcg                                                                                                            <  2:244245/149‑1 (MQ=255)                                       tCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGgcg                                                                                                            >  1:208091/1‑149 (MQ=255)                                            gCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGATAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGc                                                                                                       >  4:268437/1‑149 (MQ=255)                                             cTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCg                                                                                                                             <  6:128726/126‑1 (MQ=255)                                             cTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCg                                                                                                                             >  5:128726/1‑126 (MQ=255)                                             cTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGcc                                                                                                      >  6:175662/1‑149 (MQ=255)                                                   actacAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCgctgct                                                                                                >  2:177350/1‑149 (MQ=255)                                                     tacAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGATAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGa                                                                                              <  3:268437/149‑1 (MQ=255)                                                     tacAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAATGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCgctgct                                                                                                >  1:120222/1‑147 (MQ=255)                                                     tacAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCgctgct                                                                                                >  7:255494/1‑147 (MQ=255)                                                     tacAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGa                                                                                              <  2:174918/149‑1 (MQ=255)                                                       cAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAg                                                                                             >  1:115434/1‑148 (MQ=255)                                                          gAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAAc                                                                                         >  7:226156/1‑149 (MQ=255)                                                                ccAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGtctt                                                                                   >  8:50442/1‑149 (MQ=255)                                                                 cAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGATCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGtcttc                                                                                  >  4:223998/1‑149 (MQ=255)                                                                  aGCAACTGAACGAAGTGCGTTCTGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAAc                                                                                                                       <  4:41936/111‑1 (MQ=255)                                                                  aGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAAc                                                                                                                       >  3:41936/1‑111 (MQ=255)                                                                   gCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTtctc                                                                                <  1:177350/149‑1 (MQ=255)                                                                      aCTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCg                                                                             <  4:365152/149‑1 (MQ=255)                                                                        tGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCg                                                                             >  6:341335/1‑147 (MQ=255)                                                                        tGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCg                                                                             >  6:84112/1‑147 (MQ=255)                                                                        tGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTa                                                                           <  7:291019/149‑1 (MQ=255)                                                                         gAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAg                                                                          >  7:185560/1‑149 (MQ=255)                                                                          aaCGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTa                                                                           <  2:208091/147‑1 (MQ=255)                                                                          aaCGAAGTGCGTTCGGTTGTGCCGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTa                                                                           <  8:70673/147‑1 (MQ=255)                                                                          aaCGAAGTGCGTTCGGTTGTGCCGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTa                                                                           <  2:112988/147‑1 (MQ=255)                                                                             gAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCg                                                                                                                                                >  5:129088/1‑75 (MQ=255)                                                                             gAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCg                                                                                                                                                <  6:129088/75‑1 (MQ=255)                                                                                 tGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTTTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTc                                                                  <  8:115727/149‑1 (MQ=255)                                                                                     ttCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCcac                                                               >  5:245778/1‑148 (MQ=255)                                                                                      tCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCcac                                                               >  2:226113/1‑147 (MQ=255)                                                                                       cGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACaa                                                             <  5:84112/148‑1 (MQ=255)                                                                                                  gaagaaCAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTc                                                   <  1:226113/147‑1 (MQ=255)                                                                                                     gaaCAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCt                                              <  2:120222/149‑1 (MQ=255)                                                                                                       aCAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCgctgct                                                                                                <  4:345866/97‑1 (MQ=255)                                                                                                       aCAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCgctgct                                                                                                >  3:345866/1‑97 (MQ=255)                                                                                                       aCAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTgg                                            >  4:302723/1‑149 (MQ=255)                                                                                                        cAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTg                                             <  1:52923/147‑1 (MQ=255)                                                                                                        cAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTg                                             >  2:52923/1‑147 (MQ=255)                                                                                                                   tACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTAt                                 <  8:121557/148‑1 (MQ=255)                                                                                                                        ttCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCaa                             <  8:226156/147‑1 (MQ=255)                                                                                                                        ttCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCaa                             <  6:282899/147‑1 (MQ=255)                                                                                                                        ttCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGc                           <  7:50442/149‑1 (MQ=255)                                                                                                                        ttCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGc                           >  3:151889/1‑149 (MQ=255)                                                                                                                        ttCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTGGTGCGTATTCAAGc                           >  5:56205/1‑149 (MQ=255)                                                                                                                          cTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCa                         >  7:135759/1‑149 (MQ=255)                                                                                                                             cAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCa                         <  3:560/146‑1 (MQ=255)                                                                                                                              aGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGAc                     >  5:290074/1‑149 (MQ=255)                                                                                                                               gAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACg                                                                                        <  1:350302/81‑1 (MQ=255)                                                                                                                               gAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACg                                                                                        >  2:350302/1‑81 (MQ=255)                                                                                                                                aaaGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTa                   >  5:325203/1‑149 (MQ=255)                                                                                                                                      tacgagACCGACTTGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAagccagcc                                                      <  3:191451/103‑1 (MQ=255)                                                                                                                                      tacgagACCGACTTGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAagccagcc                                                      >  4:191451/6‑108 (MQ=255)                                                                                                                                       cTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGa            <  4:151889/149‑1 (MQ=255)                                                                                                                                       cTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGa            <  5:341335/149‑1 (MQ=255)                                                                                                                                       acgagACCGACTTGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTgg              >  3:228294/5‑147 (MQ=255)                                                                                                                                        tgtgACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAg           >  2:347134/1‑149 (MQ=255)                                                                                                                                        tgtgACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAg           >  5:330019/1‑149 (MQ=255)                                                                                                                                         gagACCGACTTGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGt          >  3:162698/3‑149 (MQ=255)                                                                                                                                         gagACCGACTTGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGt          >  3:168324/3‑149 (MQ=255)                                                                                                                                             ccGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAgc      <  6:56205/149‑1 (MQ=255)                                                                                                                                             ccGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAgc      >  2:30648/1‑149 (MQ=255)                                                                                                                                              cGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTtgtg                                      <  6:155066/116‑1 (MQ=255)                                                                                                                                              cGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTtgtg                                      >  5:155066/1‑116 (MQ=255)                                                                                                                                               gACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAgcgc    <  3:223998/149‑1 (MQ=255)                                                                                                                                                aCAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAgcg     >  2:81706/1‑147 (MQ=255)                                                                                                                                                 cAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAg  <  2:115434/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAG  >  NC_002947/2628043‑2628335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑