| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,734,030 | A→G | 7.0% | N310D (AAC→GAC) | PP_2394 → | leucine‑rich repeat‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,734,030 | 0 | A | G | 7.0% | 162.5 / 2.8 | 57 | N310D (AAC→GAC) | PP_2394 | leucine‑rich repeat‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (25/28); new base G (2/2); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
TGCTGTTCAACACCAGCAAGCGCGTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTCGAACACCTTGCTGACGACTG > NC_002947/2733887‑2734178 | tGCTGTTCAACACCAGCAAGCGCGTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGc > 1:182088/1‑149 (MQ=255) gTTCAACACCAGCAAGCGCGTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCg > 7:89231/1‑149 (MQ=255) ttCAACACCAGCAAGCGCGTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTc < 4:301914/147‑1 (MQ=255) ttCAACACCAGCAAGCGCGTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTc < 7:276202/147‑1 (MQ=255) ttCAACACCAGCAAGCGCGTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGt < 8:13341/149‑1 (MQ=255) acCAGCAAGCGCGTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCg > 5:318057/1‑149 (MQ=255) cAGCAAGCGCGTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGAGCCGAc > 8:243399/1‑149 (MQ=255) gcgTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGc < 6:318057/149‑1 (MQ=255) gTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCa < 5:370272/149‑1 (MQ=255) cTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGc > 1:247479/1‑148 (MQ=255) tACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATtcttcttg > 1:125157/1‑147 (MQ=255) ccGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGc < 6:170466/149‑1 (MQ=255) cGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCGACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGcc > 6:60000/1‑149 (MQ=255) cGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCGACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGcc > 6:54357/1‑149 (MQ=255) tGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATTCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGATTACCGGCGCTTCTTCAACCGCTTCGTTCGCCGACGCGATAGCCAg < 1:79293/130‑1 (MQ=255) tGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCa < 2:247479/149‑1 (MQ=255) tGACCCGCACGGACAATGGAGCGTGCGCAGCGACCTGGAAGATTATGACCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACAGGCGCTTCTTCAACCTCTTCGTGCTCCGACGAGATAGCCAg > 2:79293/1‑130 (MQ=255) cccGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTg < 2:182088/149‑1 (MQ=255) ccGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGCAAAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGt > 1:107857/1‑148 (MQ=255) ccGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGc < 7:52049/127‑1 (MQ=255) ccGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGc > 8:52049/1‑127 (MQ=255) ccGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGt > 5:187245/1‑147 (MQ=255) ccGCACGGCCCCTGGAGCGTACGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGtag > 8:217801/1‑147 (MQ=255) gCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAgc < 1:35100/149‑1 (MQ=255) ggCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCt > 8:262643/1‑149 (MQ=255) gAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCGACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGAc < 5:60000/148‑1 (MQ=255) gAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCGACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGAc < 5:54357/148‑1 (MQ=255) gAGCGGGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGAGGACTACCGGGGCTTCTTCAACCGCTTCGTGCGCAGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGAc < 4:46197/148‑1 (MQ=255) gCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGa < 4:337955/113‑1 (MQ=255) gCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGa > 3:337955/1‑113 (MQ=255) tGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCa < 5:20964/126‑1 (MQ=255) tGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCa > 6:20964/1‑126 (MQ=255) cgcAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAg < 7:243399/149‑1 (MQ=255) cgcAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAg < 5:46437/149‑1 (MQ=255) cAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCt > 2:288846/1‑149 (MQ=255) cGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTggg > 3:21095/1‑149 (MQ=255) tGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGAttcttc > 1:301083/1‑104 (MQ=255) tGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGAttcttc < 2:301083/104‑1 (MQ=255) cccGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTCTGGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCg < 1:288846/148‑1 (MQ=255) cccGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCGGCTGGGCTACCCGGGACCTCGAt < 2:302136/149‑1 (MQ=255) cccGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGa < 6:187245/148‑1 (MQ=255) ccgccgGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCATCTGGGCTACCCGGGACCTCGAt > 2:342930/1‑148 (MQ=255) cgccgGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGa > 4:236583/1‑149 (MQ=255) gC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTT‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGc < 2:125157/148‑1 (MQ=255) gcgAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGcc < 8:1765/148‑1 (MQ=255) gcgAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGcc > 7:1765/1‑148 (MQ=255) aaGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGa < 7:243625/56‑1 (MQ=255) aaGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGa > 8:243625/1‑56 (MQ=255) tGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCATCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTc < 1:342930/148‑1 (MQ=255) gACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTCg > 2:8176/1‑148 (MQ=255) ggCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTCGAACACCtt > 3:315757/1‑149 (MQ=255) gcgcTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTCGAACACCTTg < 8:89231/149‑1 (MQ=255) gcgcTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTCGAACACCTTg < 4:21095/149‑1 (MQ=255) cttcAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTCGAACACCTTGCTgac < 3:236583/148‑1 (MQ=255) ttcAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATc < 3:128237/118‑1 (MQ=255) ttcAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATc > 4:128237/1‑118 (MQ=255) tcAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTCGAACACCTTGCTgacgca > 1:134280/1‑147 (MQ=255) aaCCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGc < 2:143496/110‑1 (MQ=255) aaCCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGc > 1:143496/1‑110 (MQ=255) aaCCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTCGAACACCTTGCTGACGACTg > 6:104618/1‑149 (MQ=255) | TGCTGTTCAACACCAGCAAGCGCGTGCTGGTCTATGTACCGGGTGACCCGCACGGCCCCTGGAGCGTGCGCAGCGACCTGGAAGATTATGCCCGCCGGGTGCTTGGC‑AAACGCCTGCGCGAAGATGACTACCGGCGCTTCTTCAACCGCTTCGTGCGCCGACGCGATAGCCAGCGATTCTTCTC‑GGCCGTCAGTGAGCGCCTCGATGACGTGCCCAGCTGGGCTACCCGGGACCTCGATGAGCAGACCTTCGCCTATCGCCTGCCATTGTTCGAACACCTTGCTGACGACTG > NC_002947/2733887‑2734178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |