Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 239,636 T→C 8.2% S239S (AGT→AGC) ‡ hemD → uroporphyrinogen‑III synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947239,6360TC 8.2% 171.3 / 5.4 61S239S (AGT→AGC) ‡hemDuroporphyrinogen‑III synthase
Reads supporting (aligned to +/- strand):  ref base T (31/25);  new base C (3/2);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

AGGGTTTTGAACATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAACGATAAACCGTCGGCGCAGG  >  NC_002947/239494‑239778
                                                                                                                                              |                                                                                                                                              
aGGGTTTTGAACATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACg                                                                                                                                          >  3:331032/1‑149 (MQ=255)
aGGGTTTTGAACATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACg                                                                                                                                          <  4:331032/149‑1 (MQ=255)
   gTTTTGAACATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCACTGGCGCCACGGc                                                                                                                                        >  3:14720/1‑148 (MQ=255)
    ttttGAACATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCt                                                                                                                                      >  8:269812/1‑149 (MQ=255)
    ttttGAACATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCACTGGCGCCACGGcaa                                                                                                                                      <  4:14720/149‑3 (MQ=255)
       tGAACATTTGC‑GCAGATGGCCGGTGCCGATTGGGTGCGGTTTGCCCAGTTGCCGCTGTTAGTGCCCAGCCCGCGGGTCGCCGAGCCGGCCAAGGCCGCCGGGGCCCAACCGGTTTTGGATTGCCGTGGCGGCAGTGCCCCGGCCTTGCt                                                                                                                                  >  4:179826/1‑149 (MQ=255)
        gAACATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTg                                                                                                                                    >  4:309483/1‑147 (MQ=255)
        gAACATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTg                                                                                                                                    <  3:309483/147‑1 (MQ=255)
         aaCATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCt                                                                                                                                  <  5:217537/148‑1 (MQ=255)
                 cagcagATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTg                                                                                                                                 >  8:131401/1‑141 (MQ=255)
                 cagcagATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTg                                                                                                                                 <  7:131401/141‑1 (MQ=255)
                     agATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGTGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAg                                                                                                                     <  8:13574/149‑1 (MQ=255)
                           ccGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAgc                                                                                                               <  2:261564/149‑1 (MQ=255)
                              gTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCt                                                                                                            >  3:299667/1‑149 (MQ=255)
                               tGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTg                                                                                                           <  3:179826/149‑1 (MQ=255)
                                   gATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGCTACGCTGCAcg                                                                                                       <  7:269812/149‑2 (MQ=255)
                                       ggCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGc                                                                                                    <  4:299667/148‑1 (MQ=255)
                                        gCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGCCCGCCGGGCCCGAACAGGTTGTGGATTGCCGTGGCGCCCGTGCCACGCCCTTCCTGCCAGCCGTCCTCCGAACCGCTGCACCTGcca                                                                                                  >  5:143561/1‑148 (MQ=255)
                                                  gCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGT‑GATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGgcgc                                                                                        >  6:263760/1‑148 (MQ=255)
                                                   cccAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCATTGATGGCACCCTTGAAGCGTACCGATGCACATCCCTCTTAAGgcgc                                                                                        >  4:145393/1‑148 (MQ=255)
                                                    ccAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCt                                                                                       >  4:298748/1‑148 (MQ=255)
                                                          gCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTg                                                                                 <  3:298748/148‑1 (MQ=255)
                                                             gCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATg                                                                             <  3:258325/149‑1 (MQ=255)
                                                             gCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATg                                                                             >  8:221968/1‑149 (MQ=255)
                                                                 ttCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGt                                                                                                                                                <  4:368990/78‑1 (MQ=255)
                                                                 ttCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGt                                                                                                                                                >  3:368990/1‑78 (MQ=255)
                                                                 ttCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCctc                                                                                                 <  4:32889/125‑1 (MQ=255)
                                                                 ttCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCctc                                                                                                 >  3:32889/1‑125 (MQ=255)
                                                                        ccAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGcccc                                                                  >  8:48682/1‑149 (MQ=255)
                                                                        ccAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGcccc                                                                  >  1:362806/1‑149 (MQ=255)
                                                                             ccGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTgc                                                                       <  5:125143/139‑1 (MQ=255)
                                                                             ccGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTgc                                                                       >  6:125143/1‑139 (MQ=255)
                                                                             ccGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGc                                                             <  6:125396/149‑1 (MQ=255)
                                                                                    tCGCCGAGGACGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAggat                                                      <  6:143561/149‑1 (MQ=255)
                                                                                     cGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAggatg                                                     >  4:194579/1‑149 (MQ=255)
                                                                                        cGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAggatgga                                                   >  3:161704/1‑148 (MQ=255)
                                                                                               gCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAg                                           >  6:351356/1‑149 (MQ=255)
                                                                                                 cAAGGCCGCCGGGGCCCAACAGGTTGT‑GATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCga                                        <  5:263760/149‑1 (MQ=255)
                                                                                                         ccgGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGt                                              <  1:123668/136‑1 (MQ=255)
                                                                                                         ccgGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGt                                              >  2:123668/1‑136 (MQ=255)
                                                                                                         ccgGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTc                                 <  2:362806/149‑1 (MQ=255)
                                                                                                          cgGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCt                                >  2:66552/1‑149 (MQ=255)
                                                                                                          cgGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCt                                <  2:152451/149‑1 (MQ=255)
                                                                                                           ggggCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCtt                               >  7:161261/1‑149 (MQ=255)
                                                                                                            gggCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTg                              >  4:191258/1‑149 (MQ=255)
                                                                                                              gCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTc                            >  5:371785/1‑149 (MQ=255)
                                                                                                              gCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTc                            <  7:48682/149‑1 (MQ=255)
                                                                                                                ccAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTcc                           >  2:252750/1‑148 (MQ=255)
                                                                                                                        ttGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAACGa                   >  2:246648/1‑148 (MQ=255)
                                                                                                                         tgtgGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACt                                    <  3:128829/130‑1 (MQ=255)
                                                                                                                         tgtgGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACt                                    >  4:128829/1‑130 (MQ=255)
                                                                                                                           tgGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAACGATaaa               <  3:145393/149‑1 (MQ=255)
                                                                                                                              aTTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAACGATAAACCg            >  3:181804/1‑149 (MQ=255)
                                                                                                                                  ccGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAACGATAAACCGTCgg        <  6:371785/149‑1 (MQ=255)
                                                                                                                                  ccGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAACGATAAACCGTCgg        >  8:13898/1‑149 (MQ=255)
                                                                                                                                  ccGTGGCACTGGCGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAggatg                                                     >  7:323173/1‑104 (MQ=255)
                                                                                                                                  ccGTGGCACTGGCGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAggatg                                                     <  8:323173/104‑1 (MQ=255)
                                                                                                                                  ccGTGGCACTGGCGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAACGATAAACCGTCgg        >  7:331957/1‑149 (MQ=255)
                                                                                                                                       gcgcCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCtt                               >  6:275735/1‑121 (MQ=255)
                                                                                                                                       gcgcCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCtt                               <  5:275735/121‑1 (MQ=255)
                                                                                                                                        cgcCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAACGATAAACCGTCGGCGCAgg  >  5:91302/1‑149 (MQ=255)
                                                                                                                                          ccAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAAc                     >  3:119020/1‑128 (MQ=255)
                                                                                                                                          ccAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAAc                     <  4:119020/128‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
AGGGTTTTGAACATTTGCAGCAGATGGCCGGTGCCGATTGGCTGCGGTTGGCCCAGTTGCCGCTGTTCGTGCCCAGCCCGCGGGTCGCCGAGCAGGCCAAGGCCGCCGGGGCCCAACAGGTTGTGGATTGCCGTGGCGCCAGTGCCACGGCCTTGCTGGCAGCCGTGCAGCGTAGCGCTGCACCTGCCTCTTAAGGCGCTAAGCTGAATGCGGTGCGCCCCCATGCAAAGGATGGATACGTGAGCGAGACTGTCTTGTCCAATAACGATAAACCGTCGGCGCAGG  >  NC_002947/239494‑239778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑