Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,903,783 G→C 17.3% T382S (ACT→AGT)  PP_2556 ← chromate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,903,7830GC17.3% 107.7 / 14.3 46T382S (ACT→AGT) PP_2556chromate transporter
Reads supporting (aligned to +/- strand):  ref base G (20/18);  new base C (2/6);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGCTTGTAGCGGAACAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAGGAACGCCTGGTG  >  NC_002947/2903636‑2903931
                                                                                                                                                   |                                                                                                                                                    
ccGCGCTTGTAGCGGAACAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGt                                                                                                                                                     >  2:347607/1‑149 (MQ=255)
  gcgcTTGTAGCGGAACAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGa                                                                                                                                                   <  8:31674/149‑1 (MQ=255)
     cTTGTAGCGGAACAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATcc                                                                                                                                                <  4:56881/149‑1 (MQ=255)
              gAACAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTg                                                                                                                                       <  3:87624/149‑1 (MQ=255)
                   gcgcCACAGCCGCTGCGAGAGCTATCACAGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCg                                                                                                                                  <  8:308044/149‑1 (MQ=255)
                   gcgcCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCg                                                                                                                                  <  8:313526/149‑1 (MQ=255)
                     gcCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCCCACTGACCGGGATCACTGCGGCGGt                                                                                                                                <  7:14943/149‑1 (MQ=255)
                      ccACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCCCACTGACCGGGATCACTGCGGCGGTg                                                                                                                               <  2:64416/149‑1 (MQ=255)
                          aGCCGCTGCGAGCGCTATCACGGCCGATGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGGGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCCCACTGACCGGGATCACTGCGGCGGTggtgg                                                                                                                           <  7:336663/149‑5 (MQ=37)
                          aGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAAGGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCTCCACCTCCCCACTGACCGGGATCACTGCGGCGGTggtgg                                                                                                                           <  7:341704/149‑5 (MQ=37)
                                       gcTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTtgtg                                                                                                              <  1:347607/149‑1 (MQ=255)
                                         tATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACGGCGCAAGGTTGAGGATTACCCCCACCACCGCCCCACTGACCGGGATCACTGCGGCGGTg                                                                                                                               <  1:252659/130‑1 (MQ=38)
                                         tATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACGGCGCAAGGTTGAGGATTACCCCCACCACCGCCCCACTGACCGGGATCACTGCGGCGGTg                                                                                                                               >  2:252659/1‑130 (MQ=38)
                                         tATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTggg                                                                                                            >  1:356979/1‑149 (MQ=255)
                                                gccgAGGACCAGTGGAACGCGCCATGGAACCCGTCCGGCCACAGCACGTGGTATCCGAATTACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTc                                                                                                     <  5:196374/149‑1 (MQ=255)
                                                      gACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAg                                                                                               >  4:44062/1‑149 (MQ=255)
                                                         cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGt                                                                                                                                            >  6:70324/1‑101 (MQ=255)
                                                         cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGt                                                                                                                                            <  5:70324/101‑1 (MQ=255)
                                                            tGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGcc                                                                                         >  1:377214/1‑149 (MQ=255)
                                                                    gcCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTggg                                                                                                                                     >  3:196882/1‑97 (MQ=255)
                                                                    gcCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTggg                                                                                                                                     <  4:196882/97‑1 (MQ=255)
                                                                       aTCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGa                                                                              >  3:332861/1‑149 (MQ=255)
                                                                                   cGGCCCCAGCACGTGGTAGCCGAAGAACCGCGCCAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGGGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACg                                                                  <  6:325808/149‑1 (MQ=255)
                                                                                     gCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCg                                                                                             <  2:48989/120‑1 (MQ=255)
                                                                                               gTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAgg                                                      <  4:332861/149‑1 (MQ=255)
                                                                                                    aGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAAc                                                 >  5:39511/1‑149 (MQ=255)
                                                                                                     gCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAAcc                                                <  3:44062/149‑1 (MQ=255)
                                                                                                       cGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAg                                              >  8:78345/1‑149 (MQ=255)
                                                                                                          agaaCAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGt                                            >  6:386906/1‑148 (MQ=255)
                                                                                                                  gcAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGcc                                   <  6:39511/149‑1 (MQ=255)
                                                                                                                  gcAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTATGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCa                                       >  5:235583/1‑145 (MQ=255)
                                                                                                                  gcAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTATGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCa                                       <  6:235583/145‑1 (MQ=255)
                                                                                                                   cAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGccc                                  >  4:20912/1‑149 (MQ=255)
                                                                                                                   cAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGccc                                  <  2:356979/149‑1 (MQ=255)
                                                                                                                    aaGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCg                                 >  6:161597/1‑149 (MQ=255)
                                                                                                                             gATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTg                        >  1:225077/1‑149 (MQ=255)
                                                                                                                             gATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTg                        >  3:369327/1‑149 (MQ=255)
                                                                                                                             gATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTg                        >  3:49390/1‑149 (MQ=255)
                                                                                                                             gATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTg                        >  1:42440/1‑149 (MQ=255)
                                                                                                                                 aCCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGcc                    >  8:224252/1‑149 (MQ=255)
                                                                                                                                   ccccACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAgg                                             <  5:172919/122‑1 (MQ=255)
                                                                                                                                   ccccACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGCGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAgg                                             >  6:172919/1‑122 (MQ=255)
                                                                                                                                    aagttcaccGCCCCACTGACCGGGATCACTGCGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCcgc                 >  3:87036/6‑149 (MQ=38)
                                                                                                                                       ttcaccGCCCCACTGACCGGGATCACTGCGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAg              <  4:87036/147‑1 (MQ=38)
                                                                                                                                       accaccGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAg              >  5:173236/1‑149 (MQ=255)
                                                                                                                                        ccaccGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAgg             <  6:19098/149‑1 (MQ=255)
                                                                                                                                                   gTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAGGAACGCCtggtg  <  4:369327/149‑1 (MQ=255)
                                                                                                                                                   gTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAGGAACGCCtggtg  <  3:20912/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGCGCTTGTAGCGGAACAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAGGAACGCCTGGTG  >  NC_002947/2903636‑2903931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑