Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,964,948 C→G 7.3% G560A (GGG→GCG)  PP_2595 ← ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,964,9480CG 7.3% 212.3 / 3.6 68G560A (GGG→GCG) PP_2595ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (31/32);  new base G (2/3);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

CACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCTGTG  >  NC_002947/2964803‑2965087
                                                                                                                                                 |                                                                                                                                           
cACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAACCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGc                                                                                                                                          >  3:79575/1‑149 (MQ=255)
 aCGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGc                                                                                                                                          >  3:53981/1‑148 (MQ=255)
 aCGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGc                                                                                                                                          >  4:225649/1‑148 (MQ=255)
   gAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCAt                                                                                                                                       >  1:231776/1‑149 (MQ=255)
   gAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCAt                                                                                                                                       >  5:333380/1‑149 (MQ=255)
         cGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGgcgc                                                                                                                                 <  2:79055/149‑1 (MQ=255)
           aCAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGgcgcgc                                                                                                                               <  3:225649/149‑1 (MQ=255)
             aGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAg                                                                                                                             >  5:130138/1‑149 (MQ=255)
                cAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAgtgt                                                                                                                          >  4:335895/1‑149 (MQ=255)
                  gTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGTCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCGCGCCAATGGCGGGCactatgc                                                                                                                        <  5:112698/149‑7 (MQ=255)
                     aGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCg                                                                                                                     <  2:231776/149‑1 (MQ=255)
                      ggCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCgg                                                                                                                    >  8:95921/1‑149 (MQ=255)
                      ggCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCgg                                                                                                                    >  5:265816/1‑149 (MQ=255)
                       gCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGgt                                                                                                                   <  2:217600/149‑1 (MQ=255)
                         cgcgCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGgtgt                                                                                                                 <  7:161333/149‑1 (MQ=255)
                         cgcgCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGgtgt                                                                                                                 <  7:95921/149‑1 (MQ=255)
                           cgcACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGc                                                                                                               <  3:313384/149‑1 (MQ=255)
                            gcACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGcc                                                                                                              <  8:64178/149‑1 (MQ=255)
                              acaacaCCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCtt                                                                                                            <  4:53981/149‑1 (MQ=255)
                                aacaCCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCg                                                                                                                     >  7:262457/1‑138 (MQ=255)
                                aacaCCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCg                                                                                                                     <  8:262457/138‑1 (MQ=255)
                                 acacCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATCGGCGCGCAGTGTGGCCGGTGTGCCTTGtt                                                                                                         <  5:175258/149‑1 (MQ=255)
                                  cacCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTc                                                                                                        >  4:179963/1‑149 (MQ=255)
                                    ccAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGa                                                                                                      <  4:373084/149‑1 (MQ=255)
                                     cAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGa                                                                                                      >  3:278072/1‑148 (MQ=255)
                                      aGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGAc                                                                                                     >  6:57053/1‑148 (MQ=255)
                                        gAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAAc                                                                                                  >  1:125061/1‑149 (MQ=255)
                                        gAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAAc                                                                                                  <  3:152803/149‑1 (MQ=255)
                                              gATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGccc                                                                                            <  8:25790/149‑1 (MQ=255)
                                              gATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGccc                                                                                            >  7:25790/1‑149 (MQ=255)
                                              gATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGccc                                                                                            >  2:350108/1‑149 (MQ=255)
                                              gATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGccc                                                                                            <  6:130138/149‑1 (MQ=255)
                                                  aGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGc                                                                                         <  6:265816/148‑1 (MQ=255)
                                                     cctGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTcc                                                                                     <  5:349433/147‑1 (MQ=255)
                                                        gCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGc                                                                                  >  8:246875/1‑149 (MQ=255)
                                                         cAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGca                                                                                 >  1:69559/1‑149 (MQ=255)
                                                          aaCACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGcac                                                                                >  8:29091/1‑149 (MQ=255)
                                                             acGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGcaccac                                                                             <  3:179963/149‑1 (MQ=255)
                                                               gCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGa                                                                           <  3:335895/149‑1 (MQ=255)
                                                                     gTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGt                                                                     >  1:173790/1‑149 (MQ=255)
                                                                         tgtTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGc                                                                 >  2:307289/1‑149 (MQ=255)
                                                                         tgtTCCACCCTCGGTGCCCCGATGCGCCAACCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCACGGCGTCCAGCAGCACGATATGGTCGGc                                                                 <  4:79575/149‑1 (MQ=255)
                                                                              cACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATgg                                                            <  7:29091/149‑1 (MQ=255)
                                                                                cccTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGc                                                           >  4:230042/1‑148 (MQ=255)
                                                                                     ggTGCCCCGATGCACCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACgg                                                      <  2:244573/148‑1 (MQ=255)
                                                                                      gTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGG‑CACGGTGc                                                   <  6:333380/149‑1 (MQ=255)
                                                                                         ccccGATGCGACAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCt                                                  <  7:246875/148‑1 (MQ=255)
                                                                                            cGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGc                                              <  1:350108/149‑1 (MQ=255)
                                                                                                 cgcCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGgt                                          <  2:125061/148‑1 (MQ=255)
                                                                                                   ccAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGtgtg                                       >  2:38081/1‑149 (MQ=255)
                                                                                                      gCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGa                                    >  3:321300/1‑149 (MQ=255)
                                                                                                          ttGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGac                                <  5:57053/149‑1 (MQ=255)
                                                                                                             gcggcgTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGaccac                             <  6:205462/149‑1 (MQ=255)
                                                                                                               ggcgTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGaccacc                            >  6:202496/1‑148 (MQ=255)
                                                                                                                 cgTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAg                         <  5:202496/149‑1 (MQ=255)
                                                                                                                         tGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGAAACACGATATGGTCGGCCATGGCCACCGTGCTGAGCTGGTGTGCGATGACCACAAAGGTGCAACg                 >  5:294702/1‑149 (MQ=255)
                                                                                                                           ttCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGcc               >  2:163879/1‑149 (MQ=255)
                                                                                                                               aGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATg                                                             <  6:282258/99‑1 (MQ=255)
                                                                                                                               aGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATg                                                             >  5:282258/1‑99 (MQ=255)
                                                                                                                                        gCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGgt                                          <  2:297949/109‑1 (MQ=255)
                                                                                                                                        gCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGgt                                          >  1:297949/1‑109 (MQ=255)
                                                                                                                                        gCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGaccacc                            <  2:165973/123‑1 (MQ=255)
                                                                                                                                         cATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtttg  >  3:117585/1‑148 (MQ=255)
                                                                                                                                          aTAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg  >  3:90030/1‑147 (MQ=255)
                                                                                                                                              tGCGCGCCAATGGCGGGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg  <  2:105763/143‑1 (MQ=255)
                                                                                                                                              tGCGCGCCAATGGCGGGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg  <  2:165123/143‑1 (MQ=255)
                                                                                                                                              tGCGCGCCAATGGCGGGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg  >  1:165123/1‑143 (MQ=255)
                                                                                                                                              tGCGCGCCAATGGCGGGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg  >  1:105763/1‑143 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
CACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCTGTG  >  NC_002947/2964803‑2965087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑