Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,964,960 C→G 7.8% R556P (CGC→CCC)  PP_2595 ← ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,964,9600CG 7.8% 202.0 / 4.2 63R556P (CGC→CCC) PP_2595ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (27/31);  new base G (2/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCTGTGCAGCGTCTGGGCAATCACG  >  NC_002947/2964812‑2965106
                                                                                                                                                    |                                                                                                                                                  
cGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGgcgc                                                                                                                                                    <  2:79055/149‑1 (MQ=255)
  aCAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGgcgcgc                                                                                                                                                  <  3:225649/149‑1 (MQ=255)
    aGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAg                                                                                                                                                >  5:130138/1‑149 (MQ=255)
       cAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAgtgt                                                                                                                                             >  4:335895/1‑149 (MQ=255)
         gTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGTCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCGCGCCAATGGCGGGCactatgc                                                                                                                                           <  5:112698/149‑7 (MQ=255)
            aGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCg                                                                                                                                        <  2:231776/149‑1 (MQ=255)
             ggCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCgg                                                                                                                                       >  8:95921/1‑149 (MQ=255)
             ggCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCgg                                                                                                                                       >  5:265816/1‑149 (MQ=255)
              gCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGgt                                                                                                                                      <  2:217600/149‑1 (MQ=255)
                cgcgCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGgtgt                                                                                                                                    <  7:95921/149‑1 (MQ=255)
                cgcgCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGgtgt                                                                                                                                    <  7:161333/149‑1 (MQ=255)
                  cgcACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGc                                                                                                                                  <  3:313384/149‑1 (MQ=255)
                   gcACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGcc                                                                                                                                 <  8:64178/149‑1 (MQ=255)
                     acaacaCCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCtt                                                                                                                               <  4:53981/149‑1 (MQ=255)
                       aacaCCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCg                                                                                                                                        >  7:262457/1‑138 (MQ=255)
                       aacaCCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCg                                                                                                                                        <  8:262457/138‑1 (MQ=255)
                        acacCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATCGGCGCGCAGTGTGGCCGGTGTGCCTTGtt                                                                                                                            <  5:175258/149‑1 (MQ=255)
                         cacCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTc                                                                                                                           >  4:179963/1‑149 (MQ=255)
                           ccAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGa                                                                                                                         <  4:373084/149‑1 (MQ=255)
                            cAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGa                                                                                                                         >  3:278072/1‑148 (MQ=255)
                             aGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGAc                                                                                                                        >  6:57053/1‑148 (MQ=255)
                               gAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAAc                                                                                                                     >  1:125061/1‑149 (MQ=255)
                               gAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAAc                                                                                                                     <  3:152803/149‑1 (MQ=255)
                                     gATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGccc                                                                                                               <  6:130138/149‑1 (MQ=255)
                                     gATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGccc                                                                                                               >  7:25790/1‑149 (MQ=255)
                                     gATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGccc                                                                                                               >  2:350108/1‑149 (MQ=255)
                                     gATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGccc                                                                                                               <  8:25790/149‑1 (MQ=255)
                                         aGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGc                                                                                                            <  6:265816/148‑1 (MQ=255)
                                            cctGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTcc                                                                                                        <  5:349433/147‑1 (MQ=255)
                                               gCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGc                                                                                                     >  8:246875/1‑149 (MQ=255)
                                                cAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGca                                                                                                    >  1:69559/1‑149 (MQ=255)
                                                 aaCACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGcac                                                                                                   >  8:29091/1‑149 (MQ=255)
                                                    acGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGcaccac                                                                                                <  3:179963/149‑1 (MQ=255)
                                                      gCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGa                                                                                              <  3:335895/149‑1 (MQ=255)
                                                            gTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGt                                                                                        >  1:173790/1‑149 (MQ=255)
                                                                tgtTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGc                                                                                    >  2:307289/1‑149 (MQ=255)
                                                                tgtTCCACCCTCGGTGCCCCGATGCGCCAACCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCACGGCGTCCAGCAGCACGATATGGTCGGc                                                                                    <  4:79575/149‑1 (MQ=255)
                                                                     cACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATgg                                                                               <  7:29091/149‑1 (MQ=255)
                                                                       cccTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGc                                                                              >  4:230042/1‑148 (MQ=255)
                                                                            ggTGCCCCGATGCACCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACgg                                                                         <  2:244573/148‑1 (MQ=255)
                                                                             gTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGG‑CACGGTGc                                                                      <  6:333380/149‑1 (MQ=255)
                                                                                ccccGATGCGACAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCt                                                                     <  7:246875/148‑1 (MQ=255)
                                                                                   cGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGc                                                                 <  1:350108/149‑1 (MQ=255)
                                                                                        cgcCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGgt                                                             <  2:125061/148‑1 (MQ=255)
                                                                                          ccAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGtgtg                                                          >  2:38081/1‑149 (MQ=255)
                                                                                             gCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGa                                                       >  3:321300/1‑149 (MQ=255)
                                                                                                 ttGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGac                                                   <  5:57053/149‑1 (MQ=255)
                                                                                                    gcggcgTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGaccac                                                <  6:205462/149‑1 (MQ=255)
                                                                                                      ggcgTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGaccacc                                               >  6:202496/1‑148 (MQ=255)
                                                                                                        cgTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAg                                            <  5:202496/149‑1 (MQ=255)
                                                                                                                tGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGAAACACGATATGGTCGGCCATGGCCACCGTGCTGAGCTGGTGTGCGATGACCACAAAGGTGCAACg                                    >  5:294702/1‑149 (MQ=255)
                                                                                                                  ttCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGcc                                  >  2:163879/1‑149 (MQ=255)
                                                                                                                      aGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATg                                                                                <  6:282258/99‑1 (MQ=255)
                                                                                                                      aGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATg                                                                                >  5:282258/1‑99 (MQ=255)
                                                                                                                               gCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGgt                                                             >  1:297949/1‑109 (MQ=255)
                                                                                                                               gCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGgt                                                             <  2:297949/109‑1 (MQ=255)
                                                                                                                               gCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGaccacc                                               <  2:165973/123‑1 (MQ=255)
                                                                                                                                cATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtttg                     >  3:117585/1‑148 (MQ=255)
                                                                                                                                 aTAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg                     >  3:90030/1‑147 (MQ=255)
                                                                                                                                     tGCGCGCCAATGGCGGGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg                     <  2:105763/143‑1 (MQ=255)
                                                                                                                                     tGCGCGCCAATGGCGGGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg                     <  2:165123/143‑1 (MQ=255)
                                                                                                                                     tGCGCGCCAATGGCGGGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg                     >  1:165123/1‑143 (MQ=255)
                                                                                                                                     tGCGCGCCAATGGCGGGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCtgtg                     >  1:105763/1‑143 (MQ=255)
                                                                                                                                         cGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCTGTGCAGCGTCTgg           >  1:147606/1‑149 (MQ=255)
                                                                                                                                         cGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCATGCACCACGATATGGTCTGCCATGGCCACGGTGCTGAGCTGGTGTGCTATGACCACCTAGGTGCAACGCCCATGCAGGCTGTGCAGCGTCTgg           <  3:54701/149‑1 (MQ=255)
                                                                                                                                                  cgcgcAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCTTTGCAGCGTCTGGGCAATCACg  <  4:117585/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACGGTGCTGAGCTGGTGTGCGATGACCACCAAGGTGCAACGCCCATGCAGGCTGTGCAGCGTCTGGGCAATCACG  >  NC_002947/2964812‑2965106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑