Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,012,896 C→G 8.3% L44L (CTC→CTG)  PP_2634 → cellulose synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,012,8960CG 8.3% 144.5 / 4.3 48L44L (CTC→CTG) PP_2634cellulose synthase
Reads supporting (aligned to +/- strand):  ref base C (21/23);  new base G (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

GAGAGGGTCGCGCCATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACGGCAACCTGCCTGGCG  >  NC_002947/3012751‑3013032
                                                                                                                                                 |                                                                                                                                        
gagaGGGTCGCGCCATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCggg                                                                                                                                       <  6:145262/149‑1 (MQ=255)
     gggcgcgCCATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCGAGCAAGTTGCTGGGGcacag                                                                                                                                  <  8:133264/146‑5 (MQ=255)
     ggTCGCGCCATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGCCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCa                                                                                                                                  <  7:200623/149‑1 (MQ=255)
      gTCGCGCCATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCAc                                                                                                                                 <  8:57896/149‑1 (MQ=255)
      gTCGCGCCATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGAGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCAc                                                                                                                                 <  2:202086/149‑1 (MQ=255)
       tCGCGCCATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACt                                                                                                                                >  2:333609/1‑149 (MQ=255)
        cgcgCCATGACCAGCCTGGCTTTGCTTGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCAc                                                                                                                                 <  1:34974/147‑1 (MQ=255)
        cgcgCCATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGATACGCGCTGCAGGGCGTGGGCGAAGGGGTGCTGCTGATTGACCTGTGCCACAGCAACTTGCTCGGGCTGCag                                                                                                                                 >  2:34974/1‑146 (MQ=255)
            ccATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAAc                                                                                                                           >  5:241006/1‑149 (MQ=255)
            ccATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAAc                                                                                                                           >  4:183288/1‑149 (MQ=255)
                   cAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGc                                                                                                                    <  7:22401/149‑1 (MQ=255)
                        tGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGa                                                                                                                >  6:126947/1‑148 (MQ=255)
                          gCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGAt                                                                                                               <  5:296641/147‑1 (MQ=255)
                                cATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTcc                                                                                                         >  1:259100/1‑147 (MQ=255)
                                             gAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTg                                                                                          <  3:97064/149‑1 (MQ=255)
                                                     gggCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGc                                                                                   <  7:49124/148‑1 (MQ=255)
                                                       gcgcAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCggg                                                                                <  2:259100/149‑1 (MQ=255)
                                                                  tGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCg                                                                     >  7:160636/1‑149 (MQ=255)
                                                                   gctggctgCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGcc                                                                      <  1:157058/147‑1 (MQ=255)
                                                                                ggCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCgt                                                       >  8:271370/1‑149 (MQ=255)
                                                                                ggCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCgt                                                       <  1:333609/149‑1 (MQ=255)
                                                                                 gCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCgtg                                                      <  6:212880/149‑1 (MQ=255)
                                                                                   tACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTa                                                    >  3:69525/1‑149 (MQ=255)
                                                                                               ggCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTAc                                                   >  1:295302/1‑138 (MQ=255)
                                                                                               ggCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTAc                                                   <  2:295302/138‑1 (MQ=255)
                                                                                                 cGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATCCGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGtt                                                                   <  1:263932/120‑1 (MQ=255)
                                                                                                 cGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGtt                                                                   >  2:263932/1‑120 (MQ=255)
                                                                                                     ggCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCt                                  <  5:126947/149‑1 (MQ=255)
                                                                                                      gCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCtg                                 >  6:138882/1‑149 (MQ=255)
                                                                                                       cGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCtgt                                <  6:241006/149‑1 (MQ=255)
                                                                                                       cGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCGGCACGTCAACCTGCCGCTTCATACGTCCGAGGGGTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCtgt                                <  8:65416/149‑1 (MQ=255)
                                                                                                        gAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCtgtg                               >  5:138682/1‑149 (MQ=255)
                                                                                                                      gATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGGGGTGCTCGATGGCCtgt                                >  6:153978/1‑134 (MQ=255)
                                                                                                                      gATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGGGGTGCTCGATGGCCtgt                                <  5:153978/134‑1 (MQ=255)
                                                                                                                      gATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCtgtg                               >  6:85786/1‑135 (MQ=255)
                                                                                                                      gATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCtgtg                               <  5:85786/135‑1 (MQ=255)
                                                                                                                       aTTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACgg                >  7:215763/1‑149 (MQ=255)
                                                                                                                              gcagCCCGAGCAAGTTGCTGGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCt                                           >  5:87712/4‑115 (MQ=255)
                                                                                                                              gcagCCCGAGCAAGTTGCTGGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCt                                           <  6:87712/112‑1 (MQ=255)
                                                                                                                                 gCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACGGCAAcctgcct      >  7:11309/1‑149 (MQ=255)
                                                                                                                                  cccgAGCAAGTTGCTGGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACGGCAAcctgcctg     >  8:12878/1‑149 (MQ=255)
                                                                                                                                     cAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACGGCAACCTGCCTGGCg  <  8:11309/149‑1 (MQ=255)
                                                                                                                                     cAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACGGCAACCTGCCTGGCg  >  3:239722/1‑149 (MQ=255)
                                                                                                                                     cAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACGGCAACCTGCCTGGCg  >  2:109386/1‑149 (MQ=255)
                                                                                                                                           cTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCtgctgc                       <  5:386181/122‑1 (MQ=255)
                                                                                                                                           cTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCtgctgc                       >  6:386181/1‑122 (MQ=255)
                                                                                                                                               cTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACGGCAACCTGCCTGGCg  >  2:290802/1‑139 (MQ=255)
                                                                                                                                               cTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACGGCAACCTGCCTGGCg  <  1:290802/139‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                        
GAGAGGGTCGCGCCATGACCAGCCTGGCTTTGCATGGGGTACGCGGAGGCCTGGGGCGCAGCGCCTTGCTGGCTGCATTGGGCTACGCGCTGCAGGGCGTGGGCGAACGGGTGCTGCTGATTGACCTGTGCCCCAGCAACTTGCTCGGGCTGCACTTCAACCTGCCGCTGGATACGTCCGAGGGCTGGGCGCTGGCCGAGCGGGAAGGGCGGCCGTTCAGCGATGCCGTGTACGAGGTGCTCGATGGCCTGTGCCTGCTGCCGTACGGCAACCTGCCTGGCG  >  NC_002947/3012751‑3013032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑