Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,043,569 C→A 7.9% D76E (GAC→GAA)  pstS → phosphate ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,043,5690CA 7.9% 184.3 / 5.5 63D76E (GAC→GAA) pstSphosphate ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (27/31);  new base A (2/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

CTGCAGAAAACGTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCTTACAACCTGCCGGGTAACCCTAAA  >  NC_002947/3043427‑3043713
                                                                                                                                              |                                                                                                                                                  
ctgcAGAAAACGTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCt                                                                                                                                               <  4:238931/148‑1 (MQ=255)
  gcAGAAAACGTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACAGATCTACCTGACCTGGTTCAAGGACTTCAGAAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCACGGGCAGCGGCGCGGGCGTTCCGGAAGTCCTgca                                                                                                                                            >  7:57482/1‑147 (MQ=255)
     gAAAACGTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAAc                                                                                                                                           >  5:38476/1‑147 (MQ=255)
      aaaaCGTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACGTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTAGCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACaa                                                                                                                                         <  3:166149/148‑1 (MQ=255)
      aaaaCGTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTCGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAACGGCACCGGCGCCGGCGTTAAGCACTTCCAGAACaa                                                                                                                                         >  4:166149/1‑148 (MQ=255)
       aaaCGTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGAAGTCCTGAAcgccc                                                                                                                                       <  5:244782/149‑5 (MQ=255)
       aaaCGTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGAAGTCCTGAAcgcc                                                                                                                                        <  4:157048/148‑4 (MQ=255)
           gTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGt                                                                                                                                   >  7:74922/1‑149 (MQ=255)
            ttCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTc                                                                                                                                  >  4:185119/1‑149 (MQ=255)
               ggTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCCGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGAc                                                                                                                               >  1:191965/1‑149 (MQ=255)
                gTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACt                                                                                                                              >  2:249793/1‑149 (MQ=255)
                   gACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTcg                                                                                                                           >  2:120124/1‑149 (MQ=255)
                    aCCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTcgc                                                                                                                          >  2:315519/1‑149 (MQ=255)
                    aCCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTcgc                                                                                                                          >  2:346601/1‑149 (MQ=255)
                    aCCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTcgc                                                                                                                          <  6:98930/149‑1 (MQ=255)
                    aCCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTcgc                                                                                                                          >  3:146958/1‑149 (MQ=255)
                              gcgcCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGAc                                                                                                                >  1:49863/1‑149 (MQ=255)
                                  cAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCgg                                                                                                            >  5:2535/1‑149 (MQ=255)
                                   aGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGc                                                                                                           <  8:78471/149‑1 (MQ=255)
                                    gCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGcc                                                                                                          <  6:2535/149‑1 (MQ=255)
                                    gCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGcc                                                                                                          <  1:249793/149‑1 (MQ=255)
                                         ccTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGa                                                                                                      <  1:120124/148‑1 (MQ=255)
                                          cTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGc                                                                                                    >  7:250712/1‑149 (MQ=255)
                                          cTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGc                                                                                                    >  7:343928/1‑149 (MQ=255)
                                          cTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGc                                                                                                    <  8:343928/149‑1 (MQ=255)
                                          cTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTAAGGACTTCCTGAACAAGACCGTCGACTTCGCCGACAGCGACTCGGCCATGAGc                                                                                                    >  6:23804/1‑149 (MQ=255)
                                             cACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCg                                                                                                   >  7:79958/1‑147 (MQ=255)
                                             cACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGaa                                                                                                 >  4:62150/1‑149 (MQ=255)
                                             cACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGaa                                                                                                 <  3:185119/149‑1 (MQ=255)
                                                 gATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTc                                                                                                                                  >  7:120828/1‑112 (MQ=255)
                                                 gATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTc                                                                                                                                  <  8:120828/112‑1 (MQ=255)
                                                       cctgacctgGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGAcc                                                                                                                                     >  4:79920/1‑103 (MQ=255)
                                                       cctgacctgGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGAcc                                                                                                                                     <  3:79920/103‑1 (MQ=255)
                                                       cctgacctgGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCAt                                                                                                        <  8:24185/132‑1 (MQ=255)
                                                       cctgacctgGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCAt                                                                                                        >  7:24185/1‑132 (MQ=255)
                                                       cctgacctgGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCa                                                                                              <  6:15738/142‑1 (MQ=255)
                                                       cctgacctgGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTg                                                                                       >  3:243274/1‑149 (MQ=255)
                                                        ctgacctgGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGc                                                                                      <  6:281138/149‑1 (MQ=255)
                                                          gacctgGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGcc                                                                                     >  1:358981/1‑148 (MQ=255)
                                                           acctgGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCa                                                                                    <  4:146958/148‑1 (MQ=255)
                                                               ggTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGtt                                                                               >  4:91013/1‑149 (MQ=255)
                                                                   cAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑Gc                                                                          <  6:38476/148‑1 (MQ=255)
                                                                    aaGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTACAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAAAAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCg                                                                         >  6:114738/1‑148 (MQ=255)
                                                                      ggACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAg                                                                      <  1:315519/149‑1 (MQ=255)
                                                                      ggACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAg                                                                      <  8:74922/149‑1 (MQ=255)
                                                                                aaGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAAtt                                                            <  3:91013/149‑1 (MQ=255)
                                                                                     cacGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATtgctgc                                                       <  3:62150/149‑1 (MQ=255)
                                                                                        ggCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCaa                                                    >  7:89348/1‑149 (MQ=255)
                                                                                                        gATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGaaa                                     <  2:49863/148‑1 (MQ=255)
                                                                                                        gATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGaaa                                     <  3:142137/148‑1 (MQ=255)
                                                                                                             ccAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTcc                               >  7:148979/1‑149 (MQ=255)
                                                                                                                   cAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCtt                         <  1:346601/149‑1 (MQ=255)
                                                                                                                   cAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCt                          <  2:358981/148‑1 (MQ=255)
                                                                                                                   cAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAACGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCt                          >  6:301568/1‑148 (MQ=255)
                                                                                                                    acgggCAGCGGCGCGGGCGTGCAGGCCCTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTTCTACCAATGACCGCCGGTGAAATCGTCCTGGCTTa                        <  8:57482/147‑1 (MQ=255)
                                                                                                                                 cgGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTGTCGCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCTTACAACCTGCCgg             <  1:113194/149‑1 (MQ=255)
                                                                                                                                   cttgTTCAGGAAGTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCTTACAACCTGCCGGGTaa         >  6:128137/4‑149 (MQ=255)
                                                                                                                                    gCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATtgctgc                                                       <  8:196310/102‑1 (MQ=255)
                                                                                                                                    gCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCTTACAACCTGCCGGGTAAc        <  8:89348/149‑1 (MQ=255)
                                                                                                                                     cGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCTTACAACCTGCCGGGTAAcc       <  8:250712/149‑1 (MQ=255)
                                                                                                                                      gTTCAGGAAGTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCTTACAACCTGCCGGGTAAc        <  5:128137/147‑1 (MQ=255)
                                                                                                                                          aGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAAt                                                   <  2:46977/100‑1 (MQ=255)
                                                                                                                                          aGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAACGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCTTACAACCTGCCGGGTAACCCTaaa  <  5:301568/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CTGCAGAAAACGTTCGGTTGACCGGTTCGGGCGCCAGCTTCCCTGCACCGATCTACCTGACCTGGTTCAAGGACTTCAGCAAGAACACGGCTGGCGTCACCGTCGATTACCAGTCCAAGGGCAGCGGCGCGGGCGTTCAGGACTTCCTGAACAAGACCGTCGACTTCGCCGCCAGCGACTCGGCCATGAGCGAAGCAGACATTGCCAAGGTTG‑‑GCGAAGGCGTGCAATTGCTGCCAATGACCGCCGGTGAAATCGTCCTGGCTTACAACCTGCCGGGTAACCCTAAA  >  NC_002947/3043427‑3043713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑