| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,048,585 | T→C | 8.6% | intergenic (+40/‑256) | PP_2661 → / → PP_2662 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,048,585 | 0 | T | C | 8.6% | 155.7 / 4.4 | 58 | intergenic (+40/‑256) | PP_2661/PP_2662 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (23/29); new base C (2/3); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
AGAAGGTGAAGGACGCGGTGGTGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAA > NC_002947/3048439‑3048720 | aGAAGGTGAAGGACGCGGTGGTGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCttt < 4:104804/149‑1 (MQ=255) gAAGGTGAAGGACGCGGTGGTGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTg < 1:30917/149‑1 (MQ=255) gAAGGTGAAGGACGCGGTGGTGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTg < 5:205101/149‑1 (MQ=255) ggACGCGGTGGTGGATGTGCAGGCACAGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCtt > 1:197283/1‑149 (MQ=255) ggACGCGGTGGTGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATTCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTc < 2:127526/149‑1 (MQ=255) aCGCGGTGGTGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCAAAGCAGccccaatt < 1:118869/149‑6 (MQ=255) ggtggtGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGc < 1:303754/149‑1 (MQ=255) ggtggtGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGc > 5:99022/1‑149 (MQ=255) ggtggtGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGc < 1:371994/149‑1 (MQ=255) ggtggATGTGCAGGCGC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGctcg > 4:107258/1‑146 (MQ=255) tgtgCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAACGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGc < 8:39550/140‑1 (MQ=255) tgtgCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAAAGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGc > 7:39550/1‑140 (MQ=255) tgCAGGCGC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGaa < 3:107258/149‑1 (MQ=39) tgCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGTCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGCGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGaa < 4:88830/149‑1 (MQ=255) cAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCTGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCTCGCCCGCGAAg < 1:294624/149‑1 (MQ=255) cAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAg < 2:140548/148‑1 (MQ=39) gCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGTCACTGGCTTCATGAAGACAGGAGATTCC‑GCTCAGATAATGTAATTGGGTCTGCTTTGCAGCCCTTCGCGGGAGAGCACGCGAAGGGcc > 2:363008/1‑149 (MQ=255) ctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTcgc > 7:178830/1‑118 (MQ=255) ctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTcgc < 8:178830/118‑1 (MQ=255) ctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAGGGCTGCAAagc < 4:253849/145‑1 (MQ=37) ctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAGGGCTGCAAagc > 3:253849/1‑145 (MQ=37) agcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCTCGCCCGCGAAGGGCCGCAAGGCGGCCCCGGt > 4:288689/1‑149 (MQ=255) gcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAggcggc > 6:81143/1‑142 (MQ=255) gcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAggcggc < 5:81143/142‑1 (MQ=255) gcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGtt > 7:211345/1‑149 (MQ=255) caACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGttc < 8:341915/149‑1 (MQ=255) ccGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGttcatt < 2:197283/149‑1 (MQ=255) gCACCGTTATGAAATGCCCGCTTGCCAGCGGTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGttcattca < 5:344254/149‑1 (MQ=255) gAAATGCCTGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGa > 8:273386/1‑102 (MQ=255) gAAATGCCTGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGa < 7:273386/102‑1 (MQ=255) ccGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGc < 8:211345/149‑1 (MQ=255) cGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGcc > 6:44086/1‑149 (MQ=255) cGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGc > 4:30292/1‑148 (MQ=255) gCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGTAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGcc < 8:361011/148‑1 (MQ=255) gCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGttca < 5:177577/122‑1 (MQ=255) gCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGttca > 6:177577/1‑122 (MQ=255) gCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGc < 6:99022/147‑1 (MQ=255) gCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcc > 8:242514/1‑149 (MQ=255) gTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcctg < 5:44086/149‑1 (MQ=255) gCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGTCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGc > 3:330486/1‑148 (MQ=255) gCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcctgc > 8:63736/1‑136 (MQ=255) gCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATACAATCGTATTGATACCCGCCTGCCACTcctgc < 7:63736/136‑1 (MQ=255) ccTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGTCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGtct < 4:330486/147‑1 (MQ=255) gAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGc > 1:225470/1‑116 (MQ=255) gAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGc < 2:225470/116‑1 (MQ=255) gAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcct > 1:145063/1‑127 (MQ=255) gAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcct < 2:145063/127‑1 (MQ=255) cAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGa > 6:304054/1‑128 (MQ=255) cAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGa < 5:304054/128‑1 (MQ=255) aGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGAc > 6:154068/1‑149 (MQ=255) ggAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGttcat < 2:311237/89‑1 (MQ=255) ggAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGttcaa > 1:311237/1‑88 (MQ=255) cc‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCa < 7:242514/148‑1 (MQ=255) tCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCaa > 2:135223/1‑82 (MQ=255) tCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCaa < 1:135223/82‑1 (MQ=255) aGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTAc < 5:154068/147‑1 (MQ=255) tAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCTCGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGa < 3:288689/149‑1 (MQ=255) aaTGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGAc > 7:59364/1‑149 (MQ=255) aaTTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCaaa > 1:265150/1‑148 (MQ=255) | AGAAGGTGAAGGACGCGGTGGTGGATGTGCAGGCAC‑GGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCC‑GCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAA > NC_002947/3048439‑3048720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |