Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,059,387 G→A 6.4% L222L (CTG→CTA)  PP_2671 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,059,3870GA 6.4% 186.4 / 2.2 62L222L (CTG→CTA) PP_2671sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base A (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTGCACCAGGTCTCAGGCGGGCAATTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGTGACGCAGACGGTGC  >  NC_002947/3059239‑3059529
                                                                                                                                                    |                                                                                                                                              
ccTTGCACCAGGTCTCAGGCGGGCAATTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCctg                                                                                                                                                <  7:51638/149‑1 (MQ=255)
 cTTGCACCAGGTCTCAGGCGGGCAATTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCctgc                                                                                                                                               <  6:49949/149‑1 (MQ=255)
    gCACCAGGTCTCAGGCGGGCAATTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCctgct                                                                                                                                              <  1:215474/147‑1 (MQ=255)
      aCCAGGTCTCAGGCGGGCAATTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCa                                                                                                                                          <  5:132109/149‑1 (MQ=255)
          ggcctcAGGCGGGCAATTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCGTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTgc                                                                                                                                      <  6:290930/146‑1 (MQ=255)
           gTCTCAGGCGGGCAATTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTgca                                                                                                                                     <  2:34996/149‑1 (MQ=255)
             ctcACGCGGGCAATTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTgcag                                                                                                                                    >  2:135213/1‑148 (MQ=255)
                        aaTTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCg                                                                                                                         >  2:292101/1‑148 (MQ=255)
                            gACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTaca                                                                                                                     <  3:212906/148‑1 (MQ=255)
                                  aCGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTgg                                                                                                              <  1:292101/149‑1 (MQ=255)
                                          gcgAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGAcc                                                                                                      >  7:223865/1‑149 (MQ=255)
                                          gcgAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGAcc                                                                                                      >  8:277132/1‑149 (MQ=255)
                                              ggccggccTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTg                                                                                                   >  2:209107/1‑148 (MQ=255)
                                               gcaggcgTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCa                                                                                                 <  5:207703/146‑1 (MQ=255)
                                               gcaggcgTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCa                                                                                                 <  5:202534/146‑1 (MQ=255)
                                                   gccTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTgg                                                                                                              <  3:194259/132‑1 (MQ=255)
                                                   gccTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTgg                                                                                                              >  4:194259/1‑132 (MQ=255)
                                                           gAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTgg                                                                                     >  2:191897/1‑149 (MQ=255)
                                                           gAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTgg                                                                                     <  1:209107/149‑1 (MQ=255)
                                                            aaGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTtgg                                                                                    <  2:253493/149‑1 (MQ=255)
                                                                gcgcCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGAcc                                                                                                      >  7:304358/1‑127 (MQ=255)
                                                                gcgcCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGAcc                                                                                                      <  8:304358/127‑1 (MQ=255)
                                                                     aGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACgat                                                                                             <  3:18224/131‑1 (MQ=255)
                                                                     aGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACgat                                                                                             >  4:18224/1‑131 (MQ=255)
                                                                        tGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTggc                                                                         <  1:135213/148‑1 (MQ=255)
                                                                         ggCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGCCCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTggcag                                                                       >  2:249738/1‑149 (MQ=255)
                                                                           ccAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAg                                                                                                                  >  6:362866/1‑104 (MQ=255)
                                                                           ccAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAg                                                                                                                  <  5:362866/104‑1 (MQ=255)
                                                                                   ttCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGt                                                               <  2:292264/147‑1 (MQ=255)
                                                                                   ttCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGc                                                             >  1:359722/1‑149 (MQ=255)
                                                                                   ttCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATAACTGGCAGGCATGCGt                                                               >  1:292264/1‑147 (MQ=255)
                                                                                     cAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGcc                                                            <  8:223865/148‑1 (MQ=255)
                                                                                         cGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATAc                                                                              >  7:67770/1‑126 (MQ=255)
                                                                                         cGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATAc                                                                              <  8:67770/126‑1 (MQ=255)
                                                                                         cGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTggca                                                                        <  8:29465/132‑1 (MQ=255)
                                                                                         cGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTggca                                                                        >  7:29465/1‑132 (MQ=255)
                                                                                                       ccTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTa                                         >  8:342373/1‑149 (MQ=255)
                                                                                                                 gCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCg                                >  1:187650/1‑148 (MQ=255)
                                                                                                                  cTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGaa                              <  5:151023/149‑1 (MQ=255)
                                                                                                                   tCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGTCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTggca                                                                        >  6:264871/1‑106 (MQ=255)
                                                                                                                   tCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGTCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTggca                                                                        <  5:264871/106‑1 (MQ=255)
                                                                                                                         cGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCgg                       <  7:277132/149‑1 (MQ=255)
                                                                                                                                gCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTAtggtgg                                   >  3:336603/1‑130 (MQ=255)
                                                                                                                                gCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTAtggtgg                                   >  3:342073/1‑130 (MQ=255)
                                                                                                                                gCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTAtggtgg                                   <  4:342073/130‑1 (MQ=255)
                                                                                                                                gCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTAtggtgg                                   <  4:336603/130‑1 (MQ=255)
                                                                                                                                 cccAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCctgct                                              >  7:132751/1‑118 (MQ=255)
                                                                                                                                 cccAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCctgct                                              <  8:132751/118‑1 (MQ=255)
                                                                                                                                 cccAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGt                >  2:326264/1‑148 (MQ=255)
                                                                                                                                 cccAGCTGACCCAGGCCCTGCGGGCAGTGCAGGAGCATGAGCGTAGACAGCTGGCGCAGTCCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGGGGCCGAACAGCCGGAAATGGt                <  8:268920/148‑1 (MQ=255)
                                                                                                                                  ccAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGTGa              <  1:191897/149‑1 (MQ=255)
                                                                                                                                  ccAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGTGa              >  6:36137/1‑149 (MQ=255)
                                                                                                                                         aCCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGTGACGCAGAc       >  8:269934/1‑149 (MQ=255)
                                                                                                                                         aCCCAGGCCCTACTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGt                >  6:204966/1‑140 (MQ=255)
                                                                                                                                         aCCCAGGCCCTACTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGt                <  5:204966/140‑1 (MQ=255)
                                                                                                                                         aCCCAGGCCCTACTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGt                <  5:210211/140‑1 (MQ=255)
                                                                                                                                         aCCCAGGCCCTACTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGt                >  6:210211/1‑140 (MQ=255)
                                                                                                                                           ccAGGCCCTGCTGGCAGTGGAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTggca                                                                        <  8:362245/82‑1 (MQ=255)
                                                                                                                                           ccAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTggca                                                                        >  7:362245/1‑82 (MQ=255)
                                                                                                                                           ccAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGTGACGCAGACgg     >  8:184046/1‑149 (MQ=255)
                                                                                                                                           ccAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGTGACGCAGACgg     >  5:175578/1‑149 (MQ=255)
                                                                                                                                           ccAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGTGACGCAGACgg     >  5:29874/1‑149 (MQ=255)
                                                                                                                                            cAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAAt                   <  2:344598/134‑1 (MQ=255)
                                                                                                                                            cAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAAt                   >  1:344598/1‑134 (MQ=255)
                                                                                                                                              ggCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGTGACGCAGACGGTGc  <  6:175578/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CCTTGCACCAGGTCTCAGGCGGGCAATTGACGGCACGCCTGCGCGAGGCCGGCCTGCCGGAAGCGCGCCAGTTGGCCAAACACTTCAACCGCATGGCTGCAACCCTGGAGCAGGCTCAGGCCGACAATGCCCAGCTGACCCAGGCCCTGCTGGCAGTGCAGGAGCAGGAGCGTACACAGCTGGCGCAGACCCTGCACGATGATCTTGGGCAATACCTGGCAGGCATGCGTGCCCAGCTATGCCTGCTGCGTATGGTGGCCGAACAGCCGGAAATGGTGACGCAGACGGTGC  >  NC_002947/3059239‑3059529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑