Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,065,791 A→G 5.7% D244G (GAC→GGC)  PP_2677 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,065,7910AG 5.7% 210.4 / 2.5 70D244G (GAC→GGC) PP_2677hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/33);  new base G (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

TTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACC  >  NC_002947/3065644‑3065938
                                                                                                                                                   |                                                                                                                                                   
ttCCTGCGTTCTTTCTCCACCATGCCGAGGTACACGGTGCAGAGGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCGTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGac                                                                                                                                                    <  2:62611/149‑1 (MQ=255)
   cTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCTGGacaa                                                                                                                                                  >  8:342453/1‑148 (MQ=255)
    tGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGacaa                                                                                                                                                  >  1:358365/1‑147 (MQ=255)
      aGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGacaaca                                                                                                                                                <  4:317600/147‑1 (MQ=255)
       gTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCg                                                                                                                                             <  3:29235/149‑1 (MQ=255)
         tctttctCAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCgg                                                                                                                                            <  2:358365/148‑1 (MQ=255)
             tctcAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCa                                                                                                                                          <  1:286945/146‑1 (MQ=255)
             tctcAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCa                                                                                                                                          <  7:106150/146‑1 (MQ=255)
                      tGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAgg                                                                                                                              >  2:366328/1‑149 (MQ=255)
                                cAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGcc                                                                                                                    <  2:352518/149‑1 (MQ=255)
                                    gTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGTGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGa                                                                                                                <  8:56425/149‑1 (MQ=255)
                                    gTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCt                                                                                                                  >  6:199237/1‑147 (MQ=255)
                                     ccaggctGGCCAGGCGCTGGCCATCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACCCGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAg                                                                                                               >  6:274758/7‑149 (MQ=255)
                                       cAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAgc                                                                                                              <  5:194418/148‑1 (MQ=255)
                                       aggctGGCCAGGCGCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGCCGGCGCTGCCCTGa                                                                                                                <  7:324535/142‑1 (MQ=255)
                                       aggctGGCCAGGCGCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGCCGGCGCTGCCCTGa                                                                                                                >  8:324535/5‑146 (MQ=255)
                                         gATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGcgcg                                                                                                           >  2:313982/1‑149 (MQ=255)
                                               cAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCt                                                                                                     >  7:153040/1‑149 (MQ=255)
                                               cAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCt                                                                                                     >  7:126316/1‑149 (MQ=255)
                                                  cgcCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGAt                                                                                                  <  1:313982/149‑1 (MQ=255)
                                                   gcCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGAtt                                                                                                 >  3:107062/1‑149 (MQ=255)
                                                     cTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGAttt                                                                                                >  3:84928/1‑148 (MQ=255)
                                                          cAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGATCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCt                                                                                          >  7:312342/1‑149 (MQ=255)
                                                                  aGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAg                                                                                  <  4:84928/149‑1 (MQ=255)
                                                                    cTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAgcgc                                                                                                            >  7:259028/1‑121 (MQ=255)
                                                                    cTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAgcgc                                                                                                            <  8:259028/121‑1 (MQ=255)
                                                                     tGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCa                                                                               >  8:286633/1‑149 (MQ=255)
                                                                      gTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCAc                                                                              >  8:279833/1‑149 (MQ=255)
                                                                         cccGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGt                                                                           >  2:272046/1‑149 (MQ=255)
                                                                          ccGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGacaaca                                                                                                                                                <  1:166026/79‑1 (MQ=255)
                                                                          ccGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGacaaca                                                                                                                                                >  2:171879/1‑79 (MQ=255)
                                                                          ccGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGacaaca                                                                                                                                                >  2:166026/1‑79 (MQ=255)
                                                                          ccGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGacaaca                                                                                                                                                <  1:171879/79‑1 (MQ=255)
                                                                          ccGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGt                                                                           >  6:243568/1‑148 (MQ=255)
                                                                          ccGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGt                                                                           >  1:223766/1‑148 (MQ=255)
                                                                          ccGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTc                                                                          <  1:272046/149‑1 (MQ=255)
                                                                            ggcggTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACCACAGCGTCAACCAGTTCGAGTCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCAt                                                                        <  5:267162/149‑1 (MQ=255)
                                                                                 tCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGtt                                                                   <  5:243568/149‑1 (MQ=255)
                                                                                 tCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGtt                                                                   >  3:89872/1‑149 (MQ=255)
                                                                                   aGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAAGCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTg                                                                  <  7:129205/148‑1 (MQ=255)
                                                                                    gCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGa                                                                <  8:61119/149‑1 (MQ=255)
                                                                                     cGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGAt                                                               <  4:359509/149‑1 (MQ=255)
                                                                                         aaCCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCctg                                                           <  5:199237/149‑1 (MQ=255)
                                                                                            cccAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCctg                                                           >  2:1398/1‑146 (MQ=255)
                                                                                            cccAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCctg                                                           <  1:1398/146‑1 (MQ=255)
                                                                                               aaCCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGGCAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTc                                                                          >  6:49206/1‑128 (MQ=255)
                                                                                               aaCCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGGCAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTc                                                                          >  6:43456/1‑128 (MQ=255)
                                                                                               aaCCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGGCAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTc                                                                          <  5:49206/128‑1 (MQ=255)
                                                                                               aaCCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGGCAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTc                                                                          <  5:43456/128‑1 (MQ=255)
                                                                                               aaCCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTtg                                                     <  8:153040/149‑1 (MQ=255)
                                                                                                  cTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTtgctg                                                  <  7:342453/149‑1 (MQ=255)
                                                                                                   tGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTtgctgc                                                 >  3:271833/1‑149 (MQ=255)
                                                                                                     ggCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTgctgct                                                <  7:279833/148‑1 (MQ=255)
                                                                                                      gCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCtgt                                              >  3:142760/1‑149 (MQ=255)
                                                                                                           gACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTg                                         >  2:68637/1‑149 (MQ=255)
                                                                                                            aCATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTgg                                        <  5:274758/149‑1 (MQ=255)
                                                                                                            aCATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTgg                                        <  1:366328/149‑1 (MQ=255)
                                                                                                            aCATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTg                                         <  7:286633/148‑1 (MQ=255)
                                                                                                             cATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTggg                                       <  4:271833/149‑1 (MQ=255)
                                                                                                                   gggCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGcc                                 >  7:302822/1‑149 (MQ=255)
                                                                                                                    ggCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTgggccggcag                                >  3:206467/1‑142 (MQ=255)
                                                                                                                    ggCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCg                                >  3:372575/1‑149 (MQ=255)
                                                                                                                     gCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCgg                               <  4:142760/149‑1 (MQ=255)
                                                                                                                      ccccGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGtt                                                                   <  5:238305/112‑1 (MQ=255)
                                                                                                                      ccccGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGtt                                                                   >  6:238305/1‑112 (MQ=255)
                                                                                                                        ccGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGcc                             >  3:363413/1‑148 (MQ=255)
                                                                                                                         cGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGCCCa                           <  1:68637/149‑1 (MQ=255)
                                                                                                                            gCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGCCGGCAGCCCCAGGc                        <  4:206467/149‑1 (MQ=255)
                                                                                                                              cAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGCCCAGGCt                       >  2:164860/1‑148 (MQ=255)
                                                                                                                                          tgTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCctgctg                                                        >  3:352409/1‑103 (MQ=255)
                                                                                                                                          tgTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCctgctg                                                        <  4:352409/103‑1 (MQ=255)
                                                                                                                                          tgTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCt                                    <  5:113773/123‑1 (MQ=255)
                                                                                                                                          tgTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCt                                    >  6:113773/1‑123 (MQ=255)
                                                                                                                                          tgTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGCCCAGGCTGATCTGGCGTAcc          >  7:275269/1‑149 (MQ=255)
                                                                                                                                          tgTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGCCCAGGCTGATCTGGCGTAcc          >  5:2152/1‑149 (MQ=255)
                                                                                                                                            ttGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGCCCAGGCTGATCTGGCGTACCgg        <  8:126316/149‑1 (MQ=255)
                                                                                                                                              gCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCt      >  6:276964/1‑149 (MQ=255)
                                                                                                                                                  gACAACAGCGGCAACCAGATCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGCCCAGGCTGATCTGGCGAACCGGCTcccc  >  8:325015/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCGCCTGGCCAGCCTGGAGCTGTACCCGGCGGTCAGCGAAAACCCCAACCTGGGCTTTGACATTCAGGGCCCCGGGCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACC  >  NC_002947/3065644‑3065938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑