Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,068,339 A→T 7.2% T460S (ACC→TCC)  qedH‑II → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,068,3390AT 7.2% 161.4 / 3.6 56T460S (ACC→TCC) qedH‑IIquinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (31/20);  new base T (2/2);  total (33/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

CTGGATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGC  >  NC_002947/3068212‑3068486
                                                                                                                               |                                                                                                                                                     
cTGGATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAg                                                                                                                                 <  4:21954/149‑1 (MQ=255)
   gATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGa                                                                                                                                <  4:357110/147‑1 (MQ=255)
   gATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGAc                                                                                                                               >  1:142356/1‑148 (MQ=255)
     tGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCAcc                                                                                                                                                     >  3:148643/1‑125 (MQ=255)
     tGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCAcc                                                                                                                                                     <  4:148643/125‑1 (MQ=255)
       ccGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACa                                                                                                                           <  2:142356/148‑1 (MQ=255)
        cGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGaa                                                                                                                                  >  1:345562/1‑140 (MQ=255)
        cGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGaa                                                                                                                                  <  2:345562/140‑1 (MQ=255)
                  caacaaGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGtgctgc                                                                                                               >  7:271661/1‑149 (MQ=255)
                   aacaaGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTgcg                                                                                                              >  2:32770/1‑149 (MQ=255)
                        ggACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGtgct                                                                                                                 >  8:110461/1‑141 (MQ=255)
                        ggACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGtgct                                                                                                                 <  7:110461/141‑1 (MQ=255)
                         gACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCAt                                                                                                        <  8:323755/149‑1 (MQ=255)
                              aGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCTCCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑cc                                                                                                  <  4:75575/149‑1 (MQ=255)
                              aGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCTCCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑cc                                                                                                  >  3:80778/1‑149 (MQ=255)
                              aGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCTCCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑cc                                                                                                  >  3:75575/1‑149 (MQ=255)
                              aGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCTCCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑cc                                                                                                  <  4:80778/149‑1 (MQ=255)
                              aGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑cc                                                                                                  >  8:173156/1‑149 (MQ=255)
                                  cTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGt                                                                                              <  7:173156/149‑1 (MQ=255)
                                          cGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGc                                                                                       >  1:72482/1‑148 (MQ=255)
                                            tGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAg                                                                                    >  3:14124/1‑149 (MQ=255)
                                                 tCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAgt                                                                               <  2:72482/149‑1 (MQ=255)
                                                    aaCGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGc                                                                                          >  3:146402/1‑135 (MQ=255)
                                                    aaCGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGc                                                                                          <  4:146402/135‑1 (MQ=255)
                                                    aaCGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGtgtg                                                                            >  5:162633/1‑149 (MQ=255)
                                                             ggCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGTCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACaa                                                                   <  8:271661/149‑1 (MQ=255)
                                                               cATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGa                                                                 <  4:14124/149‑1 (MQ=255)
                                                                aTGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATc                                                                                                                                                  <  6:217927/69‑1 (MQ=255)
                                                                aTGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATc                                                                                                                                                  >  5:217927/1‑69 (MQ=255)
                                                                  ggACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCg                                                                                                >  5:137496/1‑115 (MQ=255)
                                                                  ggACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCg                                                                                                <  6:137496/115‑1 (MQ=255)
                                                                         tGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGcgcc                                                       >  5:130056/1‑149 (MQ=255)
                                                                          ggAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGcgccg                                                      >  4:239809/1‑149 (MQ=255)
                                                                             tcGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCTTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACaa                                                                   <  8:66902/132‑1 (MQ=255)
                                                                             aCGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACaa                                                                   >  7:66902/1‑133 (MQ=255)
                                                                             aCGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCtg                                                   >  3:14547/1‑149 (MQ=255)
                                                                               gAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCtgtg                                                 >  2:113613/1‑149 (MQ=255)
                                                                                aaGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTgg                                                >  5:130921/1‑149 (MQ=255)
                                                                                aaGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTACACCATCAAGCCGACTAAATGAAGACTACATCGCCGTCCTGCGCGCCATCTA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCCCtgtg                                                 >  1:298430/1‑149 (MQ=255)
                                                                                   gCATCGCCTATAAGAAAGGTGCGGCGTTCCACGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCCGCGGCAAGGAAGAGTGGCGCCACAAGAACTATGCGCCGCtctcgcgc                                             >  5:281994/1‑142 (MQ=255)
                                                                                           tataAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCt                                     >  4:208600/1‑149 (MQ=255)
                                                                                             taAGAAAGGTGCGTCGATCCTCGGTGCCCGGTTCCCCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGTGGCGGTGTGCTGa                                   <  2:298430/149‑1 (MQ=255)
                                                                                              aaGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGac                                  >  8:335010/1‑149 (MQ=255)
                                                                                                gAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAg                                                                                    <  4:192640/97‑1 (MQ=255)
                                                                                                gAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAg                                                                                    >  3:192640/1‑97 (MQ=255)
                                                                                                gAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGacca                                <  3:239809/149‑1 (MQ=255)
                                                                                                  aaGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTAAGCGCCGCTGGGGGGCGGTGTGCTGaccacc                              <  1:32770/149‑1 (MQ=255)
                                                                                                      tGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCaa                            <  1:194521/147‑1 (MQ=255)
                                                                                                      tGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCaa                            >  2:194521/1‑147 (MQ=255)
                                                                                                         ggcgTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGc                        >  5:211710/1‑148 (MQ=255)
                                                                                                               ccTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTg                  <  6:162633/148‑1 (MQ=255)
                                                                                                                 tCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGgtg               >  8:30140/1‑149 (MQ=255)
                                                                                                                   ggTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGtt             >  8:332169/1‑149 (MQ=255)
                                                                                                                     tGCCGGCTTCACCATCAAGCCG‑ATCAATGAAGACTAAATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCATAGGCAAGGAAGTTTGGCGCCACAATAACTATGAGCCGCTGTGGGGCGGTGTGCTGACAACCAAGGGCAACCTGGtttta            >  2:337167/1‑147 (MQ=255)
                                                                                                                         ggCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGACGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACggg       >  6:138550/1‑149 (MQ=255)
                                                                                                                           cTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGAGCCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACggg       >  1:289359/1‑148 (MQ=255)
                                                                                                                               aCCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGc  >  2:92228/1‑148 (MQ=255)
                                                                                                                               aCCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGc  >  5:339332/1‑148 (MQ=255)
                                                                                                                               |                                                                                                                                                     
CTGGATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCG‑CTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGA‑CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGC  >  NC_002947/3068212‑3068486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑