Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 262,946 G→T 7.3% intergenic (+181/‑304) PP_0211 → / → gabD‑I hypothetical protein/succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947262,9460GT 7.3% 175.3 / 3.7 55intergenic (+181/‑304)PP_0211/gabD‑Ihypothetical protein/succinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

AAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAACACAAAAAA  >  NC_002947/262799‑263094
                                                                                                                                                   |                                                                                                                                                    
aaGCATGACGAGCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGTGTACAGGGCGCAACTGGGCATCGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAgg                                                                                                                                                     <  1:349418/149‑1 (MQ=255)
      gacgacCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTgc                                                                                                                                               >  4:272129/1‑149 (MQ=255)
         gacCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTgctga                                                                                                                                            <  8:299598/149‑1 (MQ=255)
             gCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGCCCTGATGCCGTgctg                                                                                                                                             <  6:154984/144‑1 (MQ=255)
             gCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGCCCTGATGCCGTgctg                                                                                                                                             >  5:154984/1‑144 (MQ=255)
                 aGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACCCCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGCTGACAACAGCATCATCGTTTCCGCACGGCATCAGGGCGTGCTGCGCTGCTc                                                                                                                                     >  3:238538/1‑148 (MQ=255)
                 aGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATc                                                                                                                                     >  4:44698/1‑148 (MQ=255)
                 aGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATc                                                                                                                                     >  1:62361/1‑148 (MQ=255)
                     aaCGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCt                                                                                                                                >  7:27445/1‑149 (MQ=255)
                     aaCGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTCCAGCACGCCCTGATGCCGTgctgaaa                                                                                                                                          >  5:324011/1‑137 (MQ=255)
                     aaCGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTCCAGCACGCCCTGATGCCGTgctgaaa                                                                                                                                          <  6:324011/139‑3 (MQ=255)
                         aaCTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGc                                                                                                                             >  1:377289/1‑148 (MQ=255)
                                  aGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTc                                                                                                                   <  2:62361/149‑1 (MQ=255)
                                         ccTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAgg                                                                                                                                                     <  4:99113/108‑1 (MQ=255)
                                         ccTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAgg                                                                                                                                                     >  3:99113/1‑108 (MQ=255)
                                          cTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTc                                                                                                                   <  5:165510/141‑1 (MQ=255)
                                          cTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTc                                                                                                                   >  6:165510/1‑141 (MQ=255)
                                             ctctTTGGCGACACCCCTACTGTGGTTACAGGGCGCAACTGGGCAACGGGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCATCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATc                                                                                                          <  8:358805/147‑1 (MQ=255)
                                             ctctTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATc                                                                                                          <  1:18456/147‑1 (MQ=255)
                                                tttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGc                                                                                                                                                   <  2:133950/103‑1 (MQ=255)
                                                   ggCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCg                                                                                                   <  8:27445/148‑1 (MQ=255)
                                                   ggCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCg                                                                                                   <  3:44698/148‑1 (MQ=255)
                                                           cccGAGTGAGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCAAAGGCGATCTCATACATCCCCTCCGGGAGGGCCg                                                                                          >  8:173045/1‑149 (MQ=255)
                                                                  gtgGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTcc                                                                                   <  3:272129/149‑1 (MQ=255)
                                                                      ttACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGCTGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGATGGCCGTCGGTcccccc                                                                               <  7:38571/148‑1 (MQ=255)
                                                                       tACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCt                                                                              >  4:96411/1‑149 (MQ=255)
                                                                          aGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTca                                                                           >  4:159102/1‑149 (MQ=255)
                                                                            ggCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGcc                                                                                                                            >  2:372621/1‑98 (MQ=255)
                                                                            ggCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGcc                                                                                                                            <  1:372621/98‑1 (MQ=255)
                                                                                   cTGGGCAACGTGCCAGCGTTTACGTTGCCGTTGACAACAGCATCATCGTTTCAGCTCGGAATCTGTGGGTGCTGAGCTGATAGACCTCGCCATGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttcttt                                                                  <  7:173045/149‑1 (MQ=255)
                                                                                   cTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttcttt                                                                  >  4:229348/1‑149 (MQ=255)
                                                                                   cTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttcttt                                                                  <  4:343501/149‑1 (MQ=255)
                                                                                      ggCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttctttc                                                                 <  2:256273/147‑1 (MQ=255)
                                                                                        cAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGtt                                                             <  7:28656/149‑1 (MQ=255)
                                                                                        cAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGtt                                                             <  7:23093/149‑1 (MQ=255)
                                                                                             tGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTc                                                        <  2:377289/149‑1 (MQ=255)
                                                                                               ccACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCg                                                       >  6:47973/1‑148 (MQ=255)
                                                                                                cACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGt                                                      >  5:176434/1‑148 (MQ=255)
                                                                                                cACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTc                                                     >  2:53438/1‑149 (MQ=255)
                                                                                                cACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTc                                                     >  1:36056/1‑149 (MQ=255)
                                                                                                cACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTc                                                     >  7:15704/1‑149 (MQ=255)
                                                                                                    gATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTc                                                     <  4:210721/145‑1 (MQ=255)
                                                                                                    gATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTc                                                     >  3:210721/1‑145 (MQ=255)
                                                                                                    gATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAc                                                 <  3:96411/149‑1 (MQ=255)
                                                                                                     aTTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAcc                                                >  3:329016/1‑149 (MQ=255)
                                                                                                               cGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCCCATTTCTTTCAGTTCATTCGTCTAAGCTGTACTGGcc                                      >  4:269610/1‑149 (MQ=255)
                                                                                                               cGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGcc                                      <  2:36056/149‑1 (MQ=255)
                                                                                                               cGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGcc                                      >  2:287046/1‑149 (MQ=255)
                                                                                                                    acaacaGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTAt                                  <  4:329016/148‑1 (MQ=255)
                                                                                                                      aacaGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGc                                       <  6:211070/141‑1 (MQ=255)
                                                                                                                      aacaGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGc                                       >  5:211070/1‑141 (MQ=255)
                                                                                                                         aGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTgg                            <  1:287046/149‑1 (MQ=255)
                                                                                                                                   tttCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAc                                                 <  4:304695/118‑1 (MQ=255)
                                                                                                                                   tttCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAc                                                 >  3:304695/1‑118 (MQ=255)
                                                                                                                                    ttCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCTTCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTc                 >  3:328989/1‑149 (MQ=255)
                                                                                                                                    ttCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTc                 >  5:128725/1‑149 (MQ=255)
                                                                                                                                      cAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTcc                <  8:15704/148‑1 (MQ=255)
                                                                                                                                        gCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCca              >  1:248468/1‑148 (MQ=255)
                                                                                                                                                   gggCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAc                                                 <  7:216933/102‑1 (MQ=255)
                                                                                                                                                   gggCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAc                                                 >  8:216933/1‑102 (MQ=255)
                                                                                                                                                   gggCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAACACaaaaaa  <  6:176434/149‑1 (MQ=255)
                                                                                                                                                   gggCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAACACaaaaaa  <  5:47973/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAACACAAAAAA  >  NC_002947/262799‑263094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑