| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,072,627 | C→G | 10.0% | A111A (GCC→GCG) ‡ | PP_2683 → | two‑component system sensor histidine kinase/response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,072,627 | 0 | C | G | 10.0% | 163.5 / 9.8 | 60 | A111A (GCC→GCG) ‡ | PP_2683 | two‑component system sensor histidine kinase/response regulator |
| Reads supporting (aligned to +/- strand): ref base C (24/30); new base G (3/3); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
GACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTG > NC_002947/3072481‑3072771 | gACGGCATGCGTGCGGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCg > 6:103834/1‑149 (MQ=255) cGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGt > 1:48592/1‑148 (MQ=255) ggCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGa < 6:152894/149‑1 (MQ=255) ggCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGa < 4:210290/149‑1 (MQ=255) ggCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGa < 2:48592/149‑1 (MQ=255) tGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGcac < 2:303094/149‑1 (MQ=255) gCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGca > 8:26330/1‑147 (MQ=255) gCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCACGGGCGAGcacc > 5:71848/1‑148 (MQ=255) gCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCATGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCTGTGCTCACGGGCGAGcacc > 8:287702/1‑148 (MQ=255) cGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCACGGGCGAGcaccg < 6:71848/149‑3 (MQ=255) gTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGc < 2:171294/149‑1 (MQ=255) tGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCATGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCTGTGCTCACGGGCGAGCACCGcg < 7:287702/149‑2 (MQ=255) aCCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGc < 2:174509/149‑1 (MQ=255) cTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAAc < 1:201090/149‑1 (MQ=255) gcATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTg < 1:264569/149‑1 (MQ=255) tGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGc < 1:16285/122‑1 (MQ=255) tGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGc > 2:16285/1‑122 (MQ=255) tGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTgcgg > 3:268523/1‑149 (MQ=255) tCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCACGGGCGAGCACCGc < 6:25118/119‑1 (MQ=255) tCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCACGGGCGAGCACCGc > 5:25118/1‑119 (MQ=255) cTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAg > 4:125518/1‑148 (MQ=255) gacgacCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACgg > 5:102270/1‑149 (MQ=255) gacgacCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACgg > 2:20946/1‑149 (MQ=255) cGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCAt > 7:251191/1‑149 (MQ=255) gCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCAtc > 3:14050/1‑149 (MQ=255) gCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCAtc > 4:130708/1‑149 (MQ=255) cGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGAt < 4:78145/149‑1 (MQ=255) cGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGAt < 1:378495/149‑1 (MQ=255) tGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGAtgtg > 4:272936/1‑149 (MQ=255) cTGTTTTCCCTGACTGACCTGGCCGCAAACCTTTTTGATGGCGGTGCAGAGGAGTTGCAGGGCATTACCGCGGTGCTCGCCCGTGAGCACAGACTGCACGGCTACGAAACCCGTCTTCGGCGCAAGGACGGCAGCCATCTCGAtgttc < 1:223292/148‑3 (MQ=255) tCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGaa > 5:253630/1‑149 (MQ=255) ccTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAAc < 3:125518/148‑1 (MQ=255) ctgactgaCCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACct > 7:162812/1‑149 (MQ=255) acCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCtgctgc < 6:102270/147‑1 (MQ=255) acCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTgctgct < 5:103834/148‑1 (MQ=255) tGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCaaa > 5:360349/1‑149 (MQ=255) ggCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAAc > 2:336253/1‑149 (MQ=255) gccgccAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCaaa < 3:130708/147‑1 (MQ=255) cgccAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCg > 5:54290/1‑149 (MQ=255) gccAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCgg > 3:133788/1‑149 (MQ=255) ccTGTTTGATGGCGGTGCAGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATg < 3:280203/149‑1 (MQ=255) tGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATg > 3:201752/1‑147 (MQ=255) tttGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGaa < 3:96982/147‑1 (MQ=255) tttGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAg < 7:26330/148‑1 (MQ=255) aTGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCtg > 2:170196/1‑149 (MQ=255) aTGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCt < 6:360349/148‑1 (MQ=255) gTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAg < 4:86447/149‑1 (MQ=255) tGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACg > 5:15476/1‑86 (MQ=255) tGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACg < 6:15476/86‑1 (MQ=255) cGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGt < 4:268523/149‑1 (MQ=255) agTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTc < 1:20946/149‑1 (MQ=255) ttGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAg < 7:151932/127‑1 (MQ=255) ttGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAg > 8:151932/1‑127 (MQ=255) cAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCg < 1:336253/148‑1 (MQ=255) gCCATTACGGGGGTGCTCTGCGTTGAGAACAGCCTGGACTGCTAGGAAACCCGTTTGCGGAGAAAGGACTGCAGCCATCTCTATGTGCTGATGAACCTGCTGATCAAACCGGGCCATGATGGGCTGGTGTAGGGGTTTGTCGCCGACAt < 4:133788/149‑1 (MQ=255) aTTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGTTGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTAc < 5:266165/149‑1 (MQ=255) aTTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGttt < 4:309222/135‑1 (MQ=255) aTTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGttt > 3:309222/1‑135 (MQ=255) ccGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGa < 1:170196/148‑1 (MQ=255) tCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTg > 3:332513/1‑149 (MQ=255) | GACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGATGGCGGTGCCGAGGAGTTGCAGGCCATTACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTG > NC_002947/3072481‑3072771 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |