Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,126,856	G→C	11.3%	A190G (GCC→GGC)	PP_2744 ←	ribose‑phosphate pyrophosphokinase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,126,856	0	G	C	11.3%	167.8 / 8.9	60	A190G (GCC→GGC)	PP_2744	ribose‑phosphate pyrophosphokinase family protein
Reads supporting (aligned to +/- strand):  ref base G (30/23);  new base C (4/3);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

TGCCCCGTGCTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGTTGTCGAA  >  NC_002947/3126712‑3127004                                                                                                                                                  |                                                                                                                                                     tGCCCCGTGCTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTg                                                                                                                                                  >  3:153651/1‑149 (MQ=255)   ccccGTGCTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGc                                                                                                                                                >  4:208613/1‑149 (MQ=255)   ccccGTGCTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGc                                                                                                                                                >  3:282777/1‑149 (MQ=255)     ccGTGCTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCg                                                                                                                                               >  1:318110/1‑148 (MQ=255)         gCTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGGCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCCGAGCAGTTTCGCCAGGCCCTGGCgcacc                                                                                                                                           >  5:199484/1‑145 (MQ=255)         gCTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCACCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAggg                                                                                                                                                      >  5:333782/1‑137 (MQ=255)         gCTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCACCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAggg                                                                                                                                                      <  6:333782/137‑1 (MQ=255)          cTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCACCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGTTTCGCCAGGCCCTGGCgcacct                                                                                                                                          <  5:3392/148‑6 (MQ=255)             aTCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTc                                                                                                                                      >  7:321777/1‑149 (MQ=255)              tCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTgg                                                                                                                                                 <  5:127218/137‑1 (MQ=255)              tCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTgg                                                                                                                                                 >  6:127218/1‑137 (MQ=255)                aGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGGCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCCGAGCAGTTTCGCCAGGCCCTGGCGCACCTGCtggc                                                                                                                                    <  6:199484/149‑4 (MQ=38)                        gAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAAc                                                                                                                                           >  8:74079/1‑133 (MQ=255)                        gAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAAc                                                                                                                                           <  7:74079/133‑1 (MQ=255)                            aCGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGt                                                                                                                               >  6:272336/1‑141 (MQ=255)                            aCGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGt                                                                                                                               <  5:272336/141‑1 (MQ=255)                                 gACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCAc                                                                                                                     >  7:159340/1‑146 (MQ=255)                                 gACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCAc                                                                                                                     <  8:159340/146‑1 (MQ=255)                                 gACGGTGCTGCCTGCCACGTGACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCTGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGcgcg                                                                                                                                <  6:86111/135‑1 (MQ=255)                                 gACGGTGCTGCCTGCCACGTAACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGcgcg                                                                                                                                >  5:86111/1‑135 (MQ=255)                                        ctgcctgcCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCgg                                                                                                           <  1:313767/149‑1 (MQ=255)                                         tgcctgccACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGt                                                                                                          >  7:94878/1‑149 (MQ=255)                                           cctgccACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGt                                                                                                          <  8:94878/147‑1 (MQ=255)                                               ccACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACg                                                                                                    >  3:301841/1‑149 (MQ=255)                                                     tACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGt                                                                                               >  1:281907/1‑148 (MQ=255)                                                           ccAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGcc                                                                                        <  4:153651/149‑1 (MQ=255)                                                            cAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCt                                                                                       <  2:318110/149‑1 (MQ=255)                                                             aGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTg                                                                                      >  3:308116/1‑149 (MQ=255)                                                              ggTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGc                                                                                     >  1:262767/1‑149 (MQ=255)                                                              ggTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGc                                                                                     >  3:85099/1‑149 (MQ=255)                                                                          tCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGc                                                                         >  3:200074/1‑149 (MQ=255)                                                                              gcctgcctGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTAGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCgggg                                                                     >  7:9043/1‑149 (MQ=255)                                                                               cctgcctgCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGc                                                                    <  8:321777/149‑1 (MQ=255)                                                                                   cctgcTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAg                                                                 <  7:33895/148‑1 (MQ=255)                                                                                    ctgctgGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGc                                                               <  2:262767/149‑1 (MQ=255)                                                                                          gCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTg                                                           <  4:282777/147‑1 (MQ=255)                                                                                            ggTGTTTCTCCATCAAGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGc                                                        >  8:297477/1‑148 (MQ=255)                                                                                               gtTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGaa                                                     >  6:181839/1‑148 (MQ=255)                                                                                                tttCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGaa                                                     >  3:326783/1‑147 (MQ=255)                                                                                                tttCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGaa                                                     >  3:317229/1‑147 (MQ=255)                                                                                                tttCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCAAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGaa                                                     >  4:370357/1‑147 (MQ=255)                                                                                                      catcatGTCCACCGAGACCGGTCGCGGG‑AGGAGGTGCGCCAGGGCCTGGCGAAAGTGCTCGGCGCGTTTGACGCGACGGATATCCGGTTACACGACCGTGACCGCCTGCTCACCGAGTTGCTTGGCCAGGCGTTGGGCGAATAGCt                                                <  4:157823/146‑1 (MQ=255)                                                                                                      catcatGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCt                                                <  4:85099/146‑1 (MQ=255)                                                                                                      catcatGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTgg                                              <  2:281907/148‑1 (MQ=255)                                                                                                          atGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGCGCCAGGCGTTGGGCGAATAGCTGGGTGCa                                         >  4:48786/1‑149 (MQ=255)                                                                                                                  cGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGt                                 >  3:134530/1‑149 (MQ=255)                                                                                                                   gagaCCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGtt                                <  5:181839/149‑1 (MQ=255)                                                                                                                        cGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGc                           >  4:337271/1‑149 (MQ=255)                                                                                                                         ggTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCt                          >  4:39553/1‑149 (MQ=255)                                                                                                                           tCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCg                        <  3:208613/149‑1 (MQ=255)                                                                                                                           tCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCg                        >  3:125955/1‑149 (MQ=255)                                                                                                                           tCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCg                        <  4:134530/149‑1 (MQ=255)                                                                                                                            cgcgCCGAGCAGTTTCGCCAGGCCCTGGCGCACCTGCT‑GGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCa                                                                  >  2:74978/1‑106 (MQ=21)                                                                                                                            cgcgCCGAGCAGTTTCGCCAGGCCCTGGCGCACCTGCT‑GGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCa                                                                  <  1:74978/106‑1 (MQ=21)                                                                                                                             gcgccgagcagTTTCGCCAGGCCCTGGCGCACCTGCT‑GGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCgg                       >  2:107937/7‑148 (MQ=37)                                                                                                                               gcC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTc                               <  6:253314/138‑1 (MQ=255)                                                                                                                               gcC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTc                               >  5:253314/1‑138 (MQ=255)                                                                                                                                   agcagGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCg                 <  4:200074/149‑1 (MQ=255)                                                                                                                                        tttcgcCAGGCCCTGGCGAAACAGCAAGGCGCGTTTGACGCCACCGATATACGGTGACACGACCGTGACCGCCAGCTCACCGAGTAGCGGGGCCAGGCGTTGGGGGAAAAGCTGGGTGGATTGCAGGGTCCAGCTGGGGGTTCGGTAgg            >  4:66448/4‑149 (MQ=255)                                                                                                                                           cgcCAGGGCCTGGCGAAACTGCTCGGCGCGTTAGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCg          <  8:9043/148‑1 (MQ=255)                                                                                                                                            gcCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGtt        >  4:327875/1‑149 (MQ=255)                                                                                                                                              cAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGTTGACACGACCGTGACGGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTTGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGTTGt      <  4:308116/149‑1 (MQ=255)                                                                                                                                                  gCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGTTGTCGaa  <  3:337271/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     TGCCCCGTGCTGATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGTTGTCGAA  >  NC_002947/3126712‑3127004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑