Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,126,866 A→C 7.4% F187V (TTT→GTT) ‡ PP_2744 ← ribose‑phosphate pyrophosphokinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,126,8660AC 7.4% 153.4 / 4.7 54F187V (TTT→GTT) ‡PP_2744ribose‑phosphate pyrophosphokinase family protein
Reads supporting (aligned to +/- strand):  ref base A (27/23);  new base C (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

ATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGTTGTCGAAGG  >  NC_002947/3126724‑3127006
                                                                                                                                               |                                                                                                                                            
aTCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTc                                                                                                                                        >  7:321777/1‑149 (MQ=255)
   aGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGGCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCCGAGCAGTTTCGCCAGGCCCTGGCGCACCTGCtggc                                                                                                                                      <  6:199484/149‑4 (MQ=38)
           gAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAAc                                                                                                                                             >  8:74079/1‑133 (MQ=255)
           gAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAAc                                                                                                                                             <  7:74079/133‑1 (MQ=255)
               aCGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGt                                                                                                                                 >  6:272336/1‑141 (MQ=255)
               aCGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGt                                                                                                                                 <  5:272336/141‑1 (MQ=255)
                    gACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCAc                                                                                                                       <  8:159340/146‑1 (MQ=255)
                    gACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCAc                                                                                                                       >  7:159340/1‑146 (MQ=255)
                    gACGGTGCTGCCTGCCACGTGACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCTGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGcgcg                                                                                                                                  <  6:86111/135‑1 (MQ=255)
                    gACGGTGCTGCCTGCCACGTAACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGcgcg                                                                                                                                  >  5:86111/1‑135 (MQ=255)
                           ctgcctgcCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCgg                                                                                                             <  1:313767/149‑1 (MQ=255)
                            tgcctgccACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGt                                                                                                            >  7:94878/1‑149 (MQ=255)
                              cctgccACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGt                                                                                                            <  8:94878/147‑1 (MQ=255)
                                  ccACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACg                                                                                                      >  3:301841/1‑149 (MQ=255)
                                        tACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGt                                                                                                 >  1:281907/1‑148 (MQ=255)
                                              ccAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGcc                                                                                          <  4:153651/149‑1 (MQ=255)
                                               cAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCt                                                                                         <  2:318110/149‑1 (MQ=255)
                                                aGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTg                                                                                        >  3:308116/1‑149 (MQ=255)
                                                 ggTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGc                                                                                       >  1:262767/1‑149 (MQ=255)
                                                 ggTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGc                                                                                       >  3:85099/1‑149 (MQ=255)
                                                             tCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGc                                                                           >  3:200074/1‑149 (MQ=255)
                                                                 gcctgcctGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTAGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCgggg                                                                       >  7:9043/1‑149 (MQ=255)
                                                                  cctgcctgCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGc                                                                      <  8:321777/149‑1 (MQ=255)
                                                                      cctgcTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAg                                                                   <  7:33895/148‑1 (MQ=255)
                                                                       ctgctgGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGc                                                                 <  2:262767/149‑1 (MQ=255)
                                                                             gCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTg                                                             <  4:282777/147‑1 (MQ=255)
                                                                               ggTGTTTCTCCATCAAGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGc                                                          >  8:297477/1‑148 (MQ=255)
                                                                                  gtTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGaa                                                       >  6:181839/1‑148 (MQ=255)
                                                                                   tttCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGaa                                                       >  3:326783/1‑147 (MQ=255)
                                                                                   tttCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGaa                                                       >  3:317229/1‑147 (MQ=255)
                                                                                   tttCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCAAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGaa                                                       >  4:370357/1‑147 (MQ=255)
                                                                                         catcatGTCCACCGAGACCGGTCGCGGG‑AGGAGGTGCGCCAGGGCCTGGCGAAAGTGCTCGGCGCGTTTGACGCGACGGATATCCGGTTACACGACCGTGACCGCCTGCTCACCGAGTTGCTTGGCCAGGCGTTGGGCGAATAGCt                                                  <  4:157823/146‑1 (MQ=255)
                                                                                         catcatGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCt                                                  <  4:85099/146‑1 (MQ=255)
                                                                                         catcatGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTgg                                                <  2:281907/148‑1 (MQ=255)
                                                                                             atGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGCGCCAGGCGTTGGGCGAATAGCTGGGTGCa                                           >  4:48786/1‑149 (MQ=255)
                                                                                                     cGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGt                                   >  3:134530/1‑149 (MQ=255)
                                                                                                      gagaCCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGtt                                  <  5:181839/149‑1 (MQ=255)
                                                                                                           cGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGc                             >  4:337271/1‑149 (MQ=255)
                                                                                                            ggTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCt                            >  4:39553/1‑149 (MQ=255)
                                                                                                              tCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCg                          >  3:125955/1‑149 (MQ=255)
                                                                                                              tCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCg                          <  4:134530/149‑1 (MQ=255)
                                                                                                              tCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCg                          <  3:208613/149‑1 (MQ=255)
                                                                                                               cgcgCCGAGCAGTTTCGCCAGGCCCTGGCGCACCTGCT‑GGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCa                                                                    >  2:74978/1‑106 (MQ=21)
                                                                                                               cgcgCCGAGCAGTTTCGCCAGGCCCTGGCGCACCTGCT‑GGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCa                                                                    <  1:74978/106‑1 (MQ=21)
                                                                                                                gcgccgagcagTTTCGCCAGGCCCTGGCGCACCTGCT‑GGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCgg                         >  2:107937/7‑148 (MQ=37)
                                                                                                                  gcC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTc                                 <  6:253314/138‑1 (MQ=255)
                                                                                                                  gcC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTc                                 >  5:253314/1‑138 (MQ=255)
                                                                                                                      agcagGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCg                   <  4:200074/149‑1 (MQ=255)
                                                                                                                           tttcgcCAGGCCCTGGCGAAACAGCAAGGCGCGTTTGACGCCACCGATATACGGTGACACGACCGTGACCGCCAGCTCACCGAGTAGCGGGGCCAGGCGTTGGGGGAAAAGCTGGGTGGATTGCAGGGTCCAGCTGGGGGTTCGGTAgg              >  4:66448/4‑149 (MQ=255)
                                                                                                                              cgcCAGGGCCTGGCGAAACTGCTCGGCGCGTTAGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCg            <  8:9043/148‑1 (MQ=255)
                                                                                                                               gcCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGtt          >  4:327875/1‑149 (MQ=255)
                                                                                                                                 cAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGTTGACACGACCGTGACGGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTTGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGTTGt        <  4:308116/149‑1 (MQ=255)
                                                                                                                                     gCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGTTGTCGaa    <  3:337271/149‑1 (MQ=255)
                                                                                                                                       cTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCCAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGTTGTCGAAgg  >  5:238122/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                            
ATCAGGTCGTCGAATACGATGACGGTGCTGCCTGCCACGTTACCGACCAGGTGTTCGCCGCTCAGGCCTGCCTGCTGGCGGTGTTTCTCCATCATGGCCACCGAGACCGGTCGCGCC‑AGCAGGTGCGCCAGGGCCTGGCGAAACTGCTCGGCGCGTTTGACGCCACCGATATCCGGTGACACGACCGTGACCGCCTGCTCACCGAGTAGCGGGGCCAGGCGTTGGGCGAATAGCTGGGTGCATTGCAGGTTCCAGCTCGGGATTCGGTAGGCGTTGTCGAAGG  >  NC_002947/3126724‑3127006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑