Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,143,443 G→T 7.6% A123S (GCG→TCG)  rbsA‑II → ribose ABC transporter ‑ ATP‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,143,4430GT 7.6% 205.1 / 3.7 64A123S (GCG→TCG) rbsA‑IIribose ABC transporter ‑ ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base T (2/3);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGC  >  NC_002947/3143295‑3143591
                                                                                                                                                     |                                                                                                                                                    
cccGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCcg                                                                                                                                                      >  6:220306/1‑149 (MQ=255)
      ccAGGTGGAGCGCCTGGGCATTGGCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTc                                                                                                                                                >  3:306094/1‑149 (MQ=255)
             gAGCGCCTGGGCATTGGCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGga                                                                                                                                         >  4:45812/1‑149 (MQ=255)
               gcggCTTGGCATTGGCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAGACGCGCGGCCTCGCG‑ctgctgagag                                                                                                                                       <  6:267452/149‑5 (MQ=255)
                        cATTGGCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGactact                                                                                                                                 >  5:317890/1‑146 (MQ=255)
                        cATTGGCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGactact                                                                                                                                 <  6:317890/146‑1 (MQ=255)
                         aTTGGCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCgg                                                                                                                             >  8:228575/1‑149 (MQ=255)
                            ggCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTc                                                                                                                                                <  3:85680/127‑1 (MQ=255)
                            ggCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTc                                                                                                                                                >  4:85680/1‑127 (MQ=255)
                             tcTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACGGTGTGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAGACGCGCGGCCTCGCG‑ctgctgagag                                                                                                                                       <  8:194169/134‑5 (MQ=255)
                             gCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAGACGCGCGGCCTCGCG‑ctgctgagag                                                                                                                                       >  7:194169/1‑131 (MQ=255)
                              cTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCGCCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGc                                                                                                                        >  6:255240/1‑149 (MQ=255)
                              cTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTCGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGc                                                                                                                        >  4:207238/1‑149 (MQ=255)
                                     caccaGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGcc                                                                                                                 <  3:160715/149‑1 (MQ=255)
                                        caGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGc                                                                                                              >  6:339226/1‑149 (MQ=255)
                                        caGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGc                                                                                                              <  3:356481/149‑1 (MQ=255)
                                          ggAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGa                                                                                                            <  3:107312/149‑1 (MQ=255)
                                           gAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGaa                                                                                                           >  7:107359/1‑149 (MQ=255)
                                            aaCGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAAt                                                                                                          <  3:67667/149‑1 (MQ=255)
                                               gCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATgcg                                                                                                       <  7:231256/149‑1 (MQ=255)
                                                cTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATgcgc                                                                                                      <  3:45812/149‑1 (MQ=255)
                                                    ttgCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGa                                                                                                   <  4:61642/148‑1 (MQ=255)
                                                       ccGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCgg                                                                                               <  4:306094/149‑1 (MQ=255)
                                                        cGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGt                                                                                              >  8:255645/1‑149 (MQ=255)
                                                            ccGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAAc                                                                                          >  5:78816/1‑149 (MQ=255)
                                                               tttCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACt                                                                                         >  8:31187/1‑147 (MQ=255)
                                                                         ggTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGa                                                                             <  2:316118/149‑1 (MQ=255)
                                                                            gAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGc                                                                           >  5:231957/1‑148 (MQ=255)
                                                                             aaGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGCTCAGTTCACCGATCAgcgcattcgc                                                                          <  8:360881/148‑7 (MQ=255)
                                                                              aGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAAc                                                                        <  6:231957/149‑1 (MQ=255)
                                                                                  ttGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAGACGCGCGGCCTCGCG‑ctgctgaga                                                                                                                                        <  5:114186/81‑4 (MQ=25)
                                                                                  ttGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAGACGCGCGGCCTCGCG‑ctgctgaga                                                                                                                                        >  6:114186/1‑78 (MQ=25)
                                                                                    gTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATgg                                                                  >  6:254123/1‑149 (MQ=255)
                                                                                    gTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATgg                                                                  >  1:331209/1‑149 (MQ=255)
                                                                                       cttcGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTg                                                                <  5:255240/148‑1 (MQ=255)
                                                                                       cttcGGCCATGAACGGCGATTCGGACCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAg                                                                            <  7:179242/136‑1 (MQ=255)
                                                                                       cttcGGCCATGAACGGCGATTCGGACCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAg                                                                            >  8:179242/1‑136 (MQ=255)
                                                                                        ttcGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGagca                                                                                    >  3:344342/1‑127 (MQ=255)
                                                                                        ttcGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGagca                                                                                    <  4:344342/127‑1 (MQ=255)
                                                                                           ggCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑AGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTg                                                                >  1:89203/1‑144 (MQ=255)
                                                                                                  aaCGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGag                                                                                      <  3:119066/115‑1 (MQ=255)
                                                                                                  aaCGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGag                                                                                      >  4:119066/1‑115 (MQ=255)
                                                                                                   aCGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGaa                                                                                           <  5:316808/109‑1 (MQ=255)
                                                                                                   aCGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGaa                                                                                           >  6:316808/1‑109 (MQ=255)
                                                                                                   aCGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCa                                                              >  5:100378/1‑138 (MQ=255)
                                                                                                   aCGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCa                                                              <  6:100378/138‑1 (MQ=255)
                                                                                                     ggCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCcgcgcg                                                 >  2:357652/1‑149 (MQ=255)
                                                                                                               gCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCa                                       <  2:331209/149‑1 (MQ=255)
                                                                                                                     gCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACcgc                                 >  1:252533/1‑149 (MQ=255)
                                                                                                                      cTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACcgc                                 <  8:107359/148‑1 (MQ=255)
                                                                                                                         gATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCgt                               <  7:255645/147‑1 (MQ=255)
                                                                                                                         gATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCgt                               <  5:220306/147‑1 (MQ=255)
                                                                                                                           tCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGc                           <  6:78816/149‑1 (MQ=255)
                                                                                                                               cgcTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCcgc                                                    >  5:321349/1‑120 (MQ=255)
                                                                                                                               cgcTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCcgc                                                    <  6:321349/120‑1 (MQ=255)
                                                                                                                               cgcTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGgt                       <  5:339226/149‑1 (MQ=255)
                                                                                                                               cgcTCTCAGCAG‑CGCGAGGCCGCGCGTCAGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGgt                       >  4:359217/1‑149 (MQ=255)
                                                                                                                                    tcAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTg                         >  5:138005/1‑142 (MQ=255)
                                                                                                                                    tcAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTg                         <  6:138005/142‑1 (MQ=255)
                                                                                                                                      agcag‑cGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTcga                 <  2:228525/148‑1 (MQ=255)
                                                                                                                                            cgcgAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGtt                    >  6:333424/1‑140 (MQ=255)
                                                                                                                                            cgcgAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGtt                    <  5:333424/140‑1 (MQ=255)
                                                                                                                                              cgAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAAcc           >  3:197395/1‑147 (MQ=255)
                                                                                                                                              cgAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAAcc           >  7:296103/1‑147 (MQ=255)
                                                                                                                                                   ccGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTg    <  1:357652/149‑1 (MQ=255)
                                                                                                                                                    cgcgcgTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTgg   <  4:197395/149‑1 (MQ=255)
                                                                                                                                                     gcgcgTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGc  >  1:251974/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCCGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCATCCACCAGGAACGCTTGTTGCCGGCCCGTTTCACCGTGGGTGAAGCCTTGTTCTTCGGCCATGAACGGCGATTCGGGCCCTTGCTCGATCGCCGCTCTCAGCAG‑CGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGC  >  NC_002947/3143295‑3143591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑