Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,155,385 C→T 6.9% R293H (CGC→CAC)  PP_2771 ← sigma‑54 dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,155,3850CT 6.9% 175.3 / 2.3 58R293H (CGC→CAC) PP_2771sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (33/21);  new base T (2/2);  total (35/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGAC  >  NC_002947/3155238‑3155533
                                                                                                                                                    |                                                                                                                                                    
gCGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGgc                                                                                                                                                      <  8:93356/148‑1 (MQ=255)
gCGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGgc                                                                                                                                                      <  2:310067/148‑1 (MQ=255)
 cGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGgcgc                                                                                                                                                    <  7:220453/149‑1 (MQ=255)
                    gctGTAGCGGCTCAGGCCCAGCAGCCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACt                                                                                                                                 <  7:265302/147‑1 (MQ=255)
                        ttGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGttc                                                                                                                             >  6:96325/1‑149 (MQ=255)
                          gCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGttctt                                                                                                                           >  5:82525/1‑149 (MQ=255)
                           cGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGttcttc                                                                                                                          >  6:347593/1‑149 (MQ=255)
                           cGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGttctt                                                                                                                           <  1:232783/148‑1 (MQ=255)
                             gCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCgg                                                                                                                        >  1:242248/1‑149 (MQ=255)
                              cTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGTTGCTGCGAGCCTTTCAGCGCCTGttcgaggag                                                                                                                       <  7:176858/149‑7 (MQ=37)
                              cTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGTTGCTGCGAGCCTTTCAGCGCCTGttcgaggag                                                                                                                       >  8:287521/1‑143 (MQ=37)
                              cTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGTTGCTGCGAGCCTTTCAGCGCCTGttcgaggag                                                                                                                       <  7:287521/149‑7 (MQ=37)
                              cTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGTTGCTGCGAGCCTTTCAGCGCCTGttcgaggag                                                                                                                       >  8:176858/1‑143 (MQ=37)
                                 aGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGgcg                                                                                                                      <  5:96325/147‑1 (MQ=255)
                                   gCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGgcgc                                                                                                                                                    <  2:362534/115‑1 (MQ=255)
                                   gCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGgcgc                                                                                                                                                    >  1:362534/1‑115 (MQ=255)
                                      cagcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcgg                                                                                                               >  5:75188/1‑149 (MQ=255)
                                       agcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGgcgc                                                                                                                                                    >  6:302559/1‑111 (MQ=255)
                                       agcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGgcgc                                                                                                                                                    <  5:302559/111‑1 (MQ=255)
                                       agcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcggc                                                                                                              >  3:83692/1‑149 (MQ=255)
                                       agcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcggc                                                                                                              >  6:92567/1‑149 (MQ=255)
                                              tGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCt                                                                                                        >  8:303543/1‑148 (MQ=255)
                                                   gtgtGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCgg                                                                                                  <  5:347593/149‑1 (MQ=255)
                                                     gtgCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGc                                                                                                 >  4:145502/1‑148 (MQ=255)
                                                        cACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACt                                                                                             >  4:120963/1‑149 (MQ=255)
                                                         aCCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACtt                                                                                            >  5:43497/1‑149 (MQ=255)
                                                          ccTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTg                                                                                           >  7:22217/1‑149 (MQ=255)
                                                             ggTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTcc                                                                                        >  7:109730/1‑149 (MQ=255)
                                                              gTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCt                                                                                       <  4:53561/149‑1 (MQ=255)
                                                              gTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCt                                                                                       <  6:75188/149‑1 (MQ=255)
                                                                 gCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCGTCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTg                                                                                      <  1:316192/148‑1 (MQ=255)
                                                                 gCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTgg                                                                                     <  4:83692/148‑1 (MQ=255)
                                                                  cAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGgc                                                                                    <  5:298574/148‑1 (MQ=255)
                                                                  cAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGgc                                                                                    <  8:22217/148‑1 (MQ=255)
                                                                        cAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGa                                                                                               >  1:183190/1‑131 (MQ=255)
                                                                        cAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGa                                                                                               <  2:183190/131‑1 (MQ=255)
                                                                         aGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATg                                                                            >  4:282568/1‑149 (MQ=255)
                                                                           aaaCGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATgcg                                                                          >  5:188832/1‑149 (MQ=255)
                                                                                aTAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                     >  3:220246/1‑149 (MQ=255)
                                                                                aTAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                     <  4:37015/149‑1 (MQ=255)
                                                                                aTAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                     >  3:37015/1‑149 (MQ=255)
                                                                                        tGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAgg                                                             >  7:108606/1‑149 (MQ=255)
                                                                                         gCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGgc                                                            >  7:92180/1‑149 (MQ=255)
                                                                                              gCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                     >  8:85119/1‑135 (MQ=255)
                                                                                              gCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                     <  7:85119/135‑1 (MQ=255)
                                                                                                     gTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGgacgtcgt                                                >  4:110649/1‑149 (MQ=255)
                                                                                                      tCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGgtcgtcgtc                                               <  2:242248/149‑1 (MQ=255)
                                                                                                       cc‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCtt                                                                                                                   <  7:177840/80‑1 (MQ=255)
                                                                                                       cc‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCtt                                                                                                                   >  8:177840/1‑80 (MQ=255)
                                                                                                          tCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACt                                                                                                                                 <  6:117368/64‑1 (MQ=255)
                                                                                                           cGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGAc                                                                >  1:339800/1‑128 (MQ=255)
                                                                                                               cTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCAc                                         >  3:53982/1‑147 (MQ=255)
                                                                                                                    cGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCa                                  >  3:67773/1‑149 (MQ=255)
                                                                                                                     gTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGcc                                   <  8:92180/147‑1 (MQ=255)
                                                                                                                     gTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCAt                                 >  8:38766/1‑149 (MQ=255)
                                                                                                                     gTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTAACCGGCGACTTGTCCTGCCGCTCGATGCGCAGGTGCGACAGGCGAAGGTCCTCGTCCAGCCCCAGGCCAt                                 >  6:209996/1‑149 (MQ=255)
                                                                                                                       gCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTg                               >  8:305324/1‑149 (MQ=255)
                                                                                                                       gCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTg                               >  8:310918/1‑149 (MQ=255)
                                                                                                                         aGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCg                             <  4:220246/149‑1 (MQ=255)
                                                                                                                           gccgccGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCgg                            >  6:180908/1‑148 (MQ=255)
                                                                                                                             cgccgGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGca                          >  6:198342/1‑148 (MQ=255)
                                                                                                                                       ctcctcGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAgg                <  3:145502/148‑1 (MQ=255)
                                                                                                                                          ctcGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGgtgt            >  7:127798/1‑149 (MQ=255)
                                                                                                                                                cAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGa      <  4:67773/149‑1 (MQ=255)
                                                                                                                                                 aGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGAt     <  8:108606/149‑1 (MQ=255)
                                                                                                                                                   gcgcTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTgtggt         <  4:261317/143‑1 (MQ=255)
                                                                                                                                                   gcgcTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTgtggt         >  3:261317/1‑143 (MQ=255)
                                                                                                                                                    cgcTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGAc  >  7:74606/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGATCAGTCGGGCGCGGGTGATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGAC  >  NC_002947/3155238‑3155533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑