Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,155,403 A→G 5.9% L287S (TTG→TCG)  PP_2771 ← sigma‑54 dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,155,4030AG 5.9% 203.1 / 2.3 68L287S (TTG→TCG) PP_2771sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (37/27);  new base G (2/2);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGTTTTCCAGCTCGCGGA  >  NC_002947/3155258‑3155550
                                                                                                                                                  |                                                                                                                                                   
gctGTAGCGGCTCAGGCCCAGCAGCCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACt                                                                                                                                                  <  7:265302/147‑1 (MQ=255)
    ttGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGttc                                                                                                                                              >  6:96325/1‑149 (MQ=255)
      gCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGttctt                                                                                                                                            >  5:82525/1‑149 (MQ=255)
       cGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGttcttc                                                                                                                                           >  6:347593/1‑149 (MQ=255)
       cGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGttctt                                                                                                                                            <  1:232783/148‑1 (MQ=255)
         gCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCgg                                                                                                                                         >  1:242248/1‑149 (MQ=255)
          cTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGTTGCTGCGAGCCTTTCAGCGCCTGttcgaggag                                                                                                                                        >  8:287521/1‑143 (MQ=37)
          cTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGTTGCTGCGAGCCTTTCAGCGCCTGttcgaggag                                                                                                                                        <  7:287521/149‑7 (MQ=37)
          cTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGTTGCTGCGAGCCTTTCAGCGCCTGttcgaggag                                                                                                                                        >  8:176858/1‑143 (MQ=37)
          cTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGTTGCTGCGAGCCTTTCAGCGCCTGttcgaggag                                                                                                                                        <  7:176858/149‑7 (MQ=37)
             aGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGgcg                                                                                                                                       <  5:96325/147‑1 (MQ=255)
                  cagcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcgg                                                                                                                                >  5:75188/1‑149 (MQ=255)
                   agcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcggc                                                                                                                               >  6:92567/1‑149 (MQ=255)
                   agcagGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTcggc                                                                                                                               >  3:83692/1‑149 (MQ=255)
                          tGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCt                                                                                                                         >  8:303543/1‑148 (MQ=255)
                               gtgtGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCgg                                                                                                                   <  5:347593/149‑1 (MQ=255)
                                 gtgCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGc                                                                                                                  >  4:145502/1‑148 (MQ=255)
                                    cACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACt                                                                                                              >  4:120963/1‑149 (MQ=255)
                                     aCCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACtt                                                                                                             >  5:43497/1‑149 (MQ=255)
                                      ccTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTg                                                                                                            >  7:22217/1‑149 (MQ=255)
                                         ggTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTcc                                                                                                         >  7:109730/1‑149 (MQ=255)
                                          gTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCt                                                                                                        <  6:75188/149‑1 (MQ=255)
                                          gTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCt                                                                                                        <  4:53561/149‑1 (MQ=255)
                                             gCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCCGTCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTg                                                                                                       <  1:316192/148‑1 (MQ=255)
                                             gCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTgg                                                                                                      <  4:83692/148‑1 (MQ=255)
                                              cAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGgc                                                                                                     <  8:22217/148‑1 (MQ=255)
                                              cAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGgc                                                                                                     <  5:298574/148‑1 (MQ=255)
                                                    cAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGa                                                                                                                <  2:183190/131‑1 (MQ=255)
                                                    cAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGa                                                                                                                >  1:183190/1‑131 (MQ=255)
                                                     aGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATg                                                                                             >  4:282568/1‑149 (MQ=255)
                                                       aaaCGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATgcg                                                                                           >  5:188832/1‑149 (MQ=255)
                                                            aTAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                                      <  4:37015/149‑1 (MQ=255)
                                                            aTAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                                      >  3:37015/1‑149 (MQ=255)
                                                            aTAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                                      >  3:220246/1‑149 (MQ=255)
                                                                    tGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAgg                                                                              >  7:108606/1‑149 (MQ=255)
                                                                     gCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGgc                                                                             >  7:92180/1‑149 (MQ=255)
                                                                          gCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                                      >  8:85119/1‑135 (MQ=255)
                                                                          gCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGt                                                                                      <  7:85119/135‑1 (MQ=255)
                                                                                 gTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGgacgtcgt                                                                 >  4:110649/1‑149 (MQ=255)
                                                                                  tCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGgtcgtcgtc                                                                <  2:242248/149‑1 (MQ=255)
                                                                                   cc‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCtt                                                                                                                                    <  7:177840/80‑1 (MQ=255)
                                                                                   cc‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCtt                                                                                                                                    >  8:177840/1‑80 (MQ=255)
                                                                                      tCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACt                                                                                                                                                  <  6:117368/64‑1 (MQ=255)
                                                                                       cGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGAc                                                                                 >  1:339800/1‑128 (MQ=255)
                                                                                           cTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCAc                                                          >  3:53982/1‑147 (MQ=255)
                                                                                                cGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCa                                                   >  3:67773/1‑149 (MQ=255)
                                                                                                 gTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGcc                                                    <  8:92180/147‑1 (MQ=255)
                                                                                                 gTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCAt                                                  >  8:38766/1‑149 (MQ=255)
                                                                                                 gTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTAACCGGCGACTTGTCCTGCCGCTCGATGCGCAGGTGCGACAGGCGAAGGTCCTCGTCCAGCCCCAGGCCAt                                                  >  6:209996/1‑149 (MQ=255)
                                                                                                   gCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTg                                                >  8:305324/1‑149 (MQ=255)
                                                                                                   gCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTg                                                >  8:310918/1‑149 (MQ=255)
                                                                                                     aGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCg                                              <  4:220246/149‑1 (MQ=255)
                                                                                                       gccgccGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCgg                                             >  6:180908/1‑148 (MQ=255)
                                                                                                         cgccgGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGca                                           >  6:198342/1‑148 (MQ=255)
                                                                                                                   ctcctcGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAgg                                 <  3:145502/148‑1 (MQ=255)
                                                                                                                      ctcGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGgtgt                             >  7:127798/1‑149 (MQ=255)
                                                                                                                            cAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGa                       <  4:67773/149‑1 (MQ=255)
                                                                                                                             aGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGAt                      <  8:108606/149‑1 (MQ=255)
                                                                                                                               gcgcTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTgtggt                          <  4:261317/143‑1 (MQ=255)
                                                                                                                               gcgcTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTgtggt                          >  3:261317/1‑143 (MQ=255)
                                                                                                                                cgcTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGAc                   >  7:74606/1‑149 (MQ=255)
                                                                                                                                 gcTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACg                  <  7:303543/149‑1 (MQ=255)
                                                                                                                                 gcTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACg                  >  3:292560/1‑149 (MQ=255)
                                                                                                                                     aaaGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGttt               <  5:180908/148‑1 (MQ=255)
                                                                                                                                     aaaGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGtt                <  5:92567/147‑1 (MQ=255)
                                                                                                                                       aGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGTTTTcc            <  6:188832/149‑1 (MQ=255)
                                                                                                                                       aGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGTTTTcc            <  4:292560/149‑1 (MQ=255)
                                                                                                                                           tCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGTTTTCCAGCt        >  5:345770/1‑149 (MQ=255)
                                                                                                                                              cagcaACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGTTTTCCAGCTcgc     <  8:109730/149‑1 (MQ=255)
                                                                                                                                              cagcaACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGTTTTCCAGCTcgc     <  6:345770/149‑1 (MQ=255)
                                                                                                                                                 caACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGTTTTCCAGCTCGCGGa  <  7:310918/149‑1 (MQ=255)
                                                                                                                                                 caACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGTTTTCCAGCTCGCGGa  <  7:305324/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GATGTTGCGGCTCAGGCCCAGCAGGCTGGCCGTGTGCACCTGGTTGCAGTGGCAGAAACGATAGGCACTGCGTAGCAGGCTGTCC‑TCGACCTTCTCGTGCAGGCCGCCGGCCTGCTCCTCGAACAGGCGCTGAAAGGCTCGCAGCAACTGTTCTTCGGCGCTTTCGGCGGTGCTCACCGGCGACTTGTCCTGGCGCTCGATGCGCAGGTGCGACAGGCGCAGGTCGTCGTCCTGCACCAGGCCATTGCGGCACACCAGCAGGGTGTGGTGGATGACGTTTTCCAGCTCGCGGA  >  NC_002947/3155258‑3155550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑