Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,173,466 T→G 8.5% L324R (CTG→CGG)  PP_2785 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,173,4660TG 8.5% 140.2 / 4.0 47L324R (CTG→CGG) PP_2785hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/22);  new base G (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGTCTGGTATTCGGCATGTCGCC  >  NC_002947/3173326‑3173613
                                                                                                                                            |                                                                                                                                                   
cGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGa                                                                                                                                             <  4:87370/149‑1 (MQ=255)
cGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGa                                                                                                                                             >  2:254279/1‑149 (MQ=255)
  gCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACg                                                                                                                                           >  5:103848/1‑149 (MQ=255)
  gCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACg                                                                                                                                           <  5:49503/149‑1 (MQ=255)
   ccACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACg                                                                                                                                           >  3:165863/1‑148 (MQ=255)
       aGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTg                                                                                                                                      <  7:173025/149‑1 (MQ=255)
          aTCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCGGATCTCGACGCAGTGGc                                                                                                                                    >  7:50194/1‑148 (MQ=255)
          aTCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCGGATCTCGACGCAGTGGc                                                                                                                                    >  3:181930/1‑148 (MQ=255)
                gTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATc                                                                                                                              <  5:261596/148‑1 (MQ=255)
                  aCGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGc                                                                                                                           >  8:103858/1‑149 (MQ=255)
                      aGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGgct                                                                                                                       <  6:309977/149‑1 (MQ=255)
                             tCGCCTACTTCGACAATTGGCGCGTCACCAATACTTACAGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTAGATTACCTCTTGCTGGACTGGCTGATCTCGACGCAGTTGCAACATAAGCGGCTGCTCGAc                                                                                                                >  7:180933/1‑149 (MQ=255)
                                ccTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTgg                                                                                                             <  4:165863/149‑1 (MQ=255)
                                ccTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTgg                                                                                                             <  6:65878/149‑1 (MQ=255)
                                 cTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCAGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTgg                                                                                                             >  5:255349/1‑148 (MQ=255)
                                      tCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAg                                                                                                          >  6:11647/1‑146 (MQ=255)
                                      tCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAg                                                                                                          <  5:11647/146‑1 (MQ=255)
                                        gACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCgg                                                                                                     <  6:103848/149‑1 (MQ=255)
                                         aCAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCGGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGc                                                                                                    <  4:181930/149‑1 (MQ=255)
                                         aCAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCGGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGc                                                                                                    <  8:50194/149‑1 (MQ=255)
                                          cAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCa                                                                                                   <  6:328648/149‑1 (MQ=255)
                                                   cgTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAg                                                                                           >  5:163178/1‑148 (MQ=255)
                                                     tCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGACATGGTCCAGGa                                                                                         >  3:7674/1‑148 (MQ=255)
                                                     tCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGACATGGTCCAGGa                                                                                         >  3:2411/1‑148 (MQ=255)
                                                        ccAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGTTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACaaa                                                                                     >  2:16645/1‑149 (MQ=255)
                                                        ccAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACaaa                                                                                     >  8:363403/1‑149 (MQ=255)
                                                        ccAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACaaa                                                                                     >  7:156744/1‑149 (MQ=255)
                                                         cAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAAc                                                                                    >  1:53404/1‑149 (MQ=255)
                                                                       cTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCa                                                                                                                                   <  7:310288/88‑1 (MQ=255)
                                                                       cTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCa                                                                                                                                   >  8:310288/1‑88 (MQ=255)
                                                                       cTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTc                                                                      <  7:363403/149‑1 (MQ=255)
                                                                            ggCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCAcc                                                                   >  1:174991/1‑147 (MQ=255)
                                                                            ggCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCAcc                                                                   <  2:174991/147‑1 (MQ=255)
                                                                              cAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGc                                                                                                                                    >  6:249623/1‑80 (MQ=255)
                                                                              cAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGc                                                                                                                                    <  5:249623/80‑1 (MQ=255)
                                                                              cAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCgtgt                                                               <  8:156744/149‑1 (MQ=255)
                                                                                gCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCt                                                             <  7:103858/149‑1 (MQ=255)
                                                                                    ccAGCCGGCTATGACCAAGTCGCTGCTGGGCGTCTATTACCTCTTGCGTGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCg                                                         <  8:180933/149‑1 (MQ=255)
                                                                                        aCGGCTATGACCAAGTCGCCGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAgg                                                     <  6:255349/149‑1 (MQ=255)
                                                                                                    aaGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTc                                           >  5:236512/1‑147 (MQ=255)
                                                                                                    aaGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTc                                           >  4:99146/1‑147 (MQ=255)
                                                                                                                ggCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTc                               <  6:236512/147‑1 (MQ=255)
                                                                                                                       aTTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGt                      >  7:289030/1‑149 (MQ=255)
                                                                                                                           ccTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGTCTg                   <  6:163178/148‑1 (MQ=255)
                                                                                                                               ttGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGTCTGGTAtt              >  8:92072/1‑149 (MQ=255)
                                                                                                                                        ggggtGATCTCGACGCAGTGGCAACAGCAGCGGCTGCTCGACTTGCAGCCCGGCATGTTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGTCTGGTATTCGGCATGTc     <  6:242063/145‑1 (MQ=255)
                                                                                                                                          gCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGTCTGGTATTCGGCATGTcg    <  1:254279/148‑1 (MQ=255)
                                                                                                                                           cTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGTCTGGTATTCGGCATGTcgcc  <  2:53404/149‑1 (MQ=255)
                                                                                                                                            tGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGTCTGGTATTCGGCAt        >  8:217219/1‑142 (MQ=255)
                                                                                                                                            tGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGTCTGGTATTCGGCAt        <  7:217219/142‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CGGCCACAGCATCGTGGTACGCAGTGAAGTCGCCTACTTCGACAATTGGCGCGTCACCAATCCTTACCGCGCTTACGGCAGCGACCAGACGGCTATGACCAAGTCGCTGCTGGGCGTCGATTACCTCTTGCGGGACTGGCTGATCTCGACGCAGTGGCAACATCAGCGGCTGCTCGACTGGCAGCCCGGCATGGTCCAGGACAAACGCGACGACCTGTTCACCGTGTCTGCCGAAGGCTCGCAGTTCCAGGATCGCCTCAAGACCCGTCTGGTATTCGGCATGTCGCC  >  NC_002947/3173326‑3173613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑