Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,238,145 T→G 9.9% L195R (CTG→CGG)  PP_2836 → 2‑keto‑3‑deoxyxylonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,238,1450TG 9.9% 132.0 / 4.4 49L195R (CTG→CGG) PP_28362‑keto‑3‑deoxyxylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (23/21);  new base G (3/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGACTTC  >  NC_002947/3237998‑3238290
                                                                                                                                                    |                                                                                                                                                  
gCATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTg                                                                                                                                                   >  3:217074/1‑149 (MQ=255)
 cATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGt                                                                                                                                                  <  4:126271/149‑1 (MQ=255)
      gcgcCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCcgc                                                                                                                                              >  1:84075/1‑149 (MQ=255)
                             cccGGCTCGGCTCAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCg                                                                                                                       <  3:246501/148‑1 (MQ=255)
                              ccGGCTCGGCTCAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCgg                                                                                                                      >  5:122343/1‑148 (MQ=255)
                                   tcggctcAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGAAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCa                                                                                                                >  4:49030/1‑149 (MQ=255)
                                        tcAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCAcccc                                                                                                            >  7:283431/1‑148 (MQ=255)
                                        tcAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGG‑CCAACCGCGGACAGCGGTTgggc                                                                                                                                      >  1:379288/1‑119 (MQ=255)
                                        tcAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGG‑CCAACCGCGGACAGCGGTTgggc                                                                                                                                      <  2:379288/121‑3 (MQ=255)
                                          aGGCCGAAGC‑GCTGCGCCAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGGGTCCACCAAGGCCCAACCGCGGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAg                                                                                                         <  2:84075/149‑1 (MQ=255)
                                                                     gTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCgg                                                                               >  5:50846/1‑148 (MQ=255)
                                                                     gTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCgg                                                                               >  4:147032/1‑148 (MQ=255)
                                                                     gTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCg                                                                                <  8:283431/147‑1 (MQ=255)
                                                                     gTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCCGCATCCACCCCAAGTC‑CACATGGAACAACCCAGAGcccg                                                                               >  8:143399/1‑146 (MQ=255)
                                                                      tAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCgg                                                                               >  3:24760/1‑147 (MQ=255)
                                                                        gagCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCAcccc                                                                                                            <  1:179834/117‑1 (MQ=255)
                                                                        gagCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCAcccc                                                                                                            >  2:179834/1‑117 (MQ=255)
                                                                           cAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAgtgg                                                                         <  4:24760/148‑1 (MQ=255)
                                                                             gggCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGc                                                                      >  2:196185/1‑149 (MQ=255)
                                                                              gggcTGTGGTCGCAGTTCCTTGCGGTGGGTATCGGCCCGGATGCCGAAGTGTGCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCt                                                                     <  8:146022/146‑1 (MQ=255)
                                                                              ggCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTT‑‑CCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTgg                                                                   >  7:83483/1‑149 (MQ=255)
                                                                               gCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTg                                                                    <  6:165676/149‑1 (MQ=255)
                                                                                 cTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGc                                                                  >  5:159161/1‑149 (MQ=255)
                                                                                       tCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCGGAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCgtgt                                                             >  1:315886/1‑149 (MQ=255)
                                                                                         gCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCg                                                           <  6:159161/148‑1 (MQ=255)
                                                                                           aGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCgg                                                                               >  2:333905/1‑126 (MQ=255)
                                                                                           aGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCgg                                                                               <  1:333905/126‑1 (MQ=255)
                                                                                            gTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACAGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCa                                                       <  7:143399/149‑1 (MQ=255)
                                                                                            gTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTT‑‑CCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGGGCTGGCCGTGTCGAGCAAt                                                     <  8:83483/149‑1 (MQ=255)
                                                                                                   gAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgcag                                                >  5:158861/1‑149 (MQ=255)
                                                                                                    aGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgcagc                                               <  3:147032/149‑1 (MQ=255)
                                                                                                        gggTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCgt                                                               <  6:90935/129‑1 (MQ=255)
                                                                                                        gggTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCgt                                                               >  5:90935/1‑129 (MQ=255)
                                                                                                        gggTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCAGAAgtg                                                                          <  4:256349/118‑1 (MQ=255)
                                                                                                        gggTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCAGAAgtg                                                                          >  3:256349/1‑118 (MQ=255)
                                                                                                                      aTGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGTCAGCATCAAGGGCGCCATGCTc                             <  8:32145/149‑1 (MQ=255)
                                                                                                                       tGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCg                            <  1:241758/149‑1 (MQ=255)
                                                                                                                                     ccAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAc              >  5:97097/1‑149 (MQ=255)
                                                                                                                                      cAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgcagc                                               <  7:235319/115‑1 (MQ=255)
                                                                                                                                      cAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgcagc                                               >  8:235319/1‑115 (MQ=255)
                                                                                                                                      cAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGc                          >  4:266642/1‑136 (MQ=255)
                                                                                                                                      cAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGc                          <  3:266642/136‑1 (MQ=255)
                                                                                                                                        gtggccAACCGCGGACAGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTg           >  7:266670/5‑149 (MQ=255)
                                                                                                                                          ggccAACCGCGGACAGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTgcg         >  6:156335/3‑149 (MQ=255)
                                                                                                                                          gCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTgcg         <  6:122343/149‑1 (MQ=255)
                                                                                                                                                ccGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAacg                      <  6:304190/130‑1 (MQ=255)
                                                                                                                                                ccGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAacg                      >  5:304190/1‑130 (MQ=255)
                                                                                                                                                 cGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGACTTc  >  8:97917/1‑149 (MQ=255)
                                                                                                                                                   cTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCgt                                                               <  2:251623/86‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGC‑GCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTC‑CAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGACTTC  >  NC_002947/3237998‑3238290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑