| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | 100% | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 100.0% | 140.8 / NA | 48 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (26/22); total (26/22) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 145.1 / NA | 48 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (26/22); total (26/22) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGGCA‑CG‑CCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA > NC_002947/1499338‑1499621 || ccGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGCt > 8:85506/1‑149 (MQ=255) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 6:15117/149‑1 (MQ=255) ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt < 2:61491/149‑1 (MQ=255) gTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcg < 7:264107‑M2/149‑5 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGGACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCTTGGCACCGGCTTTGCCGGTGttcgcgg > 6:380732‑M2/1‑141 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGcc < 2:113898/134‑1 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGcc > 1:113898/1‑134 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcggg > 3:7665‑M2/1‑138 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcggg > 3:12816‑M2/1‑138 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACAGGCGCCCCGGTGCATGGCACCGGCGTTGGCCGGGttcgcggg < 4:7665‑M2/146‑9 (MQ=255) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgc < 4:327613‑M2/149‑14 (MQ=255) gTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaca > 1:225580‑M2/1‑125 (MQ=255) aTGCGGCCATTCAGAGCCGGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccacagac > 7:85506‑M2/1‑122 (MQ=255) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcc > 8:353151‑M2/1‑103 (MQ=255) aaCGACGCCTTGGCACCCCACCAGCACGGCGGCTCCCAACGGGTGGGCGCGGGCGTCCCGGCTAAACAGGCGCGGCGGTGCATGGCACGGGTTTTGCCGGTGTGC‑G‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCggg < 2:301712/147‑1 (MQ=12) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAAAAGGCGCCGCGGTGCATGGCACCGGCTTTGGCGGTGT‑TAG‑CGGGCACCC‑CCG‑CGCCCCCCGACTGAGTGCCATTAATCGGGCCGAt > 2:279114/1‑149 (MQ=21) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATc < 6:389534/149‑1 (MQ=21) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAG‑CGGGCA‑CGCCCG‑CTCCCACAGACGGATTGCCATTAAGCGGGCCGATCGc > 7:114347/1‑148 (MQ=21) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTGCCGCGAAACAGGCGCCCCGGTGCATGGCACCGGCGTTTCCGGTGT‑TAG‑CGGGCACCC‑CCG‑CTCCCACAGACTGATTTGCATTTATCGGGCCGATAGCGACAGTGa > 1:20524/1‑149 (MQ=16) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGCCACCGGCTTTGCGGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 3:327613/149‑1 (MQ=21) agccgGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCGTCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc < 1:279114/149‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGAATGCCAATTAGCGGGCCTATCGCTAAAGTGACGGCCCGGTTTttgttg > 1:55164/1‑149 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc < 6:281621/149‑1 (MQ=21) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACACGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTc > 3:92515/1‑149 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCGCGAAAAACGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAG‑CGGGCA‑CGCCCG‑CGCCCACAGACAGAATTCCATTAAGCGGGGCTATGGCGACAGGGACGGCCCCTTTTTTGTTGGCACGc > 2:91235/1‑149 (MQ=12) gAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccacaga > 1:115251‑M2/1‑56 (MQ=255) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 3:124719/149‑1 (MQ=25) gggCGTGCCCTCGAAACAGGCGCCGGGGGGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 8:264107/149‑1 (MQ=37) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑TGCGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc > 4:46093/1‑149 (MQ=37) gCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 2:20524/149‑1 (MQ=37) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 7:118861/1‑149 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 8:242905/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttgtc < 5:257948‑M2/148‑105 (MQ=255) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttgtc > 6:257948‑M2/1‑44 (MQ=255) ccGCGAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 3:162970/149‑1 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggctt < 8:163576‑M2/103‑62 (MQ=255) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggctt > 7:163576‑M2/1‑42 (MQ=255) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCAGTTTTTTCTTGCCTCGCTAACGAATCATGAATCCCTTAGAAc > 4:321986/1‑149 (MQ=25) aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAGGACTCCCTTAGAACg > 2:30835/1‑148 (MQ=37) ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 7:242905/149‑1 (MQ=37) cgccgcGGTGCATGGCACCGGCTTTGCGGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 3:58649/149‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 5:276654/1‑149 (MQ=37) ccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 4:92515/149‑1 (MQ=37) gcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 8:324725/1‑146 (MQ=37) cATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 4:271625/1‑148 (MQ=38) aTGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg > 3:30067/1‑85 (MQ=17) aTGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg < 4:30067/85‑1 (MQ=17) aTGGCACCGGCTTGCCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa < 5:87569/149‑1 (MQ=38) || CCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGGCA‑CG‑CCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA > NC_002947/1499338‑1499621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |