Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,845,705	+G	intergenic (‑72/+26)	PP_3394 ← / ← PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,845,703	1	.	G	100.0%	47.8 / NA	15	intergenic (‑70/+28)	PP_3394/PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (8/7);  total (8/7)

TGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC  >  NC_002947/3845559‑3845842                                                                                                                                                  |                                                                                                                                            tgctgTCGGCATGATCACTTGCAGGCTCTGCATGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑ggg                                                                                                                                          >  2:9546/1‑147 (MQ=255)              aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGtg                                                                                                                              >  2:106088/1‑147 (MQ=255)                 gcTTGCAGAGCCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGc                                                                                                                           >  3:140420/2‑148 (MQ=255)                      cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCAc                                                                                                                        <  2:38657/146‑1 (MQ=255)                           tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGAt                                                                                                                 <  2:33086/148‑1 (MQ=255)                             tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATcc                                                                                                                                    >  1:962/1‑127 (MQ=255)                             tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTGGCTGACGATGGATCa                                                                                                               <  1:106088/148‑1 (MQ=255)                             tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCa                                                                                                               <  3:70535/148‑1 (MQ=255)                              gCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCa                                                                                                                                   <  2:962/127‑1 (MQ=255)                                      tCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGcc                                                                                                      >  8:86698/1‑148 (MQ=255)                                                           attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgca                                                                                  >  8:71221/1‑147 (MQ=255)                                                            ttgtttttgttGAAAGCGGGGGCTCTCTGCCTGAAGCTTTGCCCCATACTATCTGGTGGTGGACGGGACGAAACCACGTGTTGCGCGGGCTCACCCATCGTCACCCACGATGTAGCAGCCCGCGCCCTGCCAGGCCGGTGCACgcag                                                                                 <  6:58089/147‑1 (MQ=255)                                                                         cATCGGGGTCTCTCTGTCTCACGCTTTGCGCCACAATAAGAGCTGTATCACGGGCACAAACGCCGTTTTTCGCGGGCTTAGCCAGGGTCCGCCACGTGGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCaa                                                                   <  2:89302/148‑1 (MQ=255)                                                                                     tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgag                                                       >  3:36326/1‑148 (MQ=255)                                                                                                                        tGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATACCTCGTCCGCCACGCTGGATCAGCCCGCGCCCGGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCCGGCGCCGCGCCGCCAGCGAGAGCGTGTGCCGGTCGCGCAt                        >  2:15534/1‑144 (MQ=255)                                                                                                                                          tgtTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCCGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGCGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgc  >  5:121966/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                            TGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC  >  NC_002947/3845559‑3845842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑