Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,682,024	+A	coding (314/1431 nt)	mltD →	membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,682,024	1	.	A	100.0%	74.9 / NA	23	F105Y (TTC→TAC)	mltD	membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (12/11);  total (12/11)

ACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTT‑CATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCAC  >  NC_002947/4681878‑4682158                                                                                                                                                    |                                                                                                                                       aCCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCtt                                                                                                                                         >  8:74814/1‑147 (MQ=255)     tCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCttacat                                                                                                                                     >  8:6625/1‑143 (MQ=255)       cccGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGCGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCg                                                                                                                                   >  3:75034/1‑147 (MQ=255)                           ccgAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCa                                                                                                              <  6:22290/148‑1 (MQ=255)                                  ggCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTa                                                                                                       >  4:186/1‑148 (MQ=255)                                     gccgcAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCt                                                                                                    <  2:7541/148‑1 (MQ=255)                                            gATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTAc                                                                                             >  8:10895/1‑148 (MQ=255)                                                 cTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACAtcgt                                                                                        <  7:6625/148‑1 (MQ=255)                                                  tGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGCCAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCCACCCGCCCTTACATCGAAAGCGCCGGGGACCGTGGCAGCCTCTACCTGCCCTACAtcgtc                                                                                       >  1:150489/1‑148 (MQ=255)                                                     gAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGa                                                                                     <  7:74814/147‑1 (MQ=255)                                                           aTGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCtt                                                                              <  1:91956/148‑1 (MQ=255)                                                                ccAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGCACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCGTgaaga                                                                         >  7:35712/1‑148 (MQ=255)                                                                     gCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAAcgcg                                                                    >  5:6496/1‑148 (MQ=255)                                                                      cTTCGCCCTGCAGGACAGTATCGATGGCTACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCAGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGa                                                                   <  8:132173/148‑1 (MQ=255)                                                                       ttCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGAc                                                                  >  1:9337/1‑148 (MQ=255)                                                                            ccTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGcc                                                             >  6:77413/1‑148 (MQ=255)                                                                              tGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCGACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGc                                                           >  5:94343/1‑148 (MQ=255)                                                                                            gATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCctg                                             >  2:101443/1‑148 (MQ=255)                                                                                                 cAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCtgctgc                                         <  4:75034/147‑1 (MQ=255)                                                                                                        cgcATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATc                                 <  2:99980/148‑1 (MQ=255)                                                                                                           aTCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCg                                >  4:131050/1‑146 (MQ=255)                                                                                                              gAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGt                           >  2:39350/1‑148 (MQ=255)                                                                                                                              ggTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATgg           <  3:2807/148‑1 (MQ=255)                                                                                                                              ggTTCGCCAGCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATg            <  2:9337/147‑1 (MQ=255)                                                                                                                                       gCAACCCGACCTTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCAc  <  8:84913/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                       ACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCGCTTGTGGTTCGCCAGCAACCCGACCTT‑CATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCAC  >  NC_002947/4681878‑4682158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑