Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	191,125	C→T	V83M (GTG→ATG)	PP_0166 ←	HlyD family secretion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	191,125	0	C	T	100.0%	91.0 / NA	28	V83M (GTG→ATG)	PP_0166	HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/11);  total (17/11)

GTCAGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCACCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTCGATCAGGG  >  NC_002947/190985‑191268                                                                                                                                             |                                                                                                                                                gTCAGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTg                                                                                                                                             >  7:100090/1‑145 (MQ=255)       cTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGa                                                                                                                                    <  8:49010/148‑1 (MQ=255)           gTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAAt                                                                                                                                  <  8:100090/146‑1 (MQ=255)                               gAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTa                                                                                                            >  2:10410/1‑148 (MQ=255)                                  gCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACt                                                                                                         >  8:129244/1‑148 (MQ=255)                                    gggTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTtc                                                                                                       <  6:93126/148‑1 (MQ=255)                                           aTCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATg                                                                                                >  2:146344/1‑148 (MQ=255)                                           aTCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATg                                                                                                >  2:5491/1‑148 (MQ=255)                                             ccAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATggg                                                                                              >  4:136232/1‑148 (MQ=255)                                                              tggcCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCAGGCCCAt                                                                              >  7:31364/1‑147 (MQ=255)                                                                  ccACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACaa                                                                          >  2:127413/1‑147 (MQ=255)                                                                  ccACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAAt                                                                         <  4:46986/148‑1 (MQ=255)                                                                     cTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCaa                                                                      <  3:104515/148‑1 (MQ=255)                                                                      ttCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCaa                                                                      >  2:70964/1‑147 (MQ=255)                                                                         gacgaTCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGaa                                                                   <  8:54113/147‑1 (MQ=255)                                                                         gacgaTCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGa                                                                    <  1:70964/146‑1 (MQ=255)                                                                            gaTCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAacac                                                                >  3:136957/1‑147 (MQ=255)                                                                                                  aTTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGCACAGGATCACGCCCCAGa                                         >  2:9266/1‑148 (MQ=255)                                                                                                  aTTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGa                                         >  2:11572/1‑148 (MQ=255)                                                                                                   tttCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGCACACGCAGAACAGGATCACGCCCCAGAt                                        >  5:16453/1‑148 (MQ=255)                                                                                                   tttCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGAt                                        <  3:2199/148‑1 (MQ=255)                                                                                                      cGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATgg                                      <  5:33144/147‑1 (MQ=255)                                                                                                                  cgccCTCCAGGTTCTGGATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGcaccac                         >  3:120686/1‑148 (MQ=255)                                                                                                                                  gATCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTCg         >  7:107981/1‑148 (MQ=255)                                                                                                                                   aTCTTCTGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGATCACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTCGa        <  1:146344/148‑1 (MQ=255)                                                                                                                                   aTCTTCTGCATCTTGGAAGACGGAATGGACTTTCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGATGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCtt           <  5:90043/145‑1 (MQ=255)                                                                                                                                    tcttctGCATCTTGGAAGACGGAATGGCCATGCCTTCACCCCGCCTAACTTCGTCGATGGGCGCGACGCAGGCCAATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGAAGGTCAGCCGCACCACGCGCGGTGCAACTTCGa        >  8:102347/1‑147 (MQ=255)                                                                                                                                         tGCATCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTCGATCAggg  >  2:41309/1‑148 (MQ=255)                                                                                                                                             |                                                                                                                                                GTCAGCCTCGGTTTCACCAACGTTGGAGGCGAAGCGGGTTTCATCCAGGCGCAGCAATGGCTGGCCCACTTCGACGATCTGCCCCTCCTTGGCGAAGATTTCGGCGACGATGCCGCCCTCCAGGTTCTGGATCTTCTGCACCTTGGAAGACGGAATGGCCTTGCCTTCACCCCGCGTAACTTCGTCGATGGGCGCGACGCTGGCCCATACAATCAAGAACACGAAGAACAGGATCACGCCCCAGATGGTCAGCCGCACCACGCGCGGTGCATCTTCGATCAGGG  >  NC_002947/190985‑191268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑