Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,621,706 G→A L194F (CTT→TTT)  PP_4940 ← glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,621,7060GA97.1% 113.7 / NA 36L194F (CTT→TTT) PP_4940glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (22/12);  minor base T (0/1);  total (22/14)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCTTGCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAGCGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGATATCACGCTCA  >  NC_002947/5621562‑5621848
                                                                                                                                                |                                                                                                                                              
tGTCTTGCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAATGAATACGGTTAAACgt                                                                                                                                             >  1:108835/1‑148 (MQ=255)
tGTCTTGCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACgt                                                                                                                                             >  8:2443/1‑148 (MQ=255)
 gTCTTGCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCTACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACgtg                                                                                                                                            <  1:63983/148‑1 (MQ=255)
      gCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCa                                                                                                                                       >  4:3383/1‑148 (MQ=255)
       cAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTg                                                                                                                                         <  2:120477/145‑1 (MQ=255)
         gACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCagta                                                                                                                                    <  6:114156/148‑1 (MQ=255)
              aGCGCACGGTTCTCTTCCTCGCCATGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCt                                                                                                                               >  3:21990/1‑148 (MQ=255)
                   aCGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGCCATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGcc                                                                                                                           >  6:138846/1‑147 (MQ=255)
                   aCGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGcc                                                                                                                           >  1:80359/1‑147 (MQ=255)
                       ttCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCCACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGa                                                                                                                        >  7:125549/1‑146 (MQ=255)
                        tctcTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACtt                                                                                                                     <  2:80359/148‑1 (MQ=255)
                          tctTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTATTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTg                                                                                                                    >  4:12926/1‑147 (MQ=255)
                               cTCGCCTTGGTCGTATGCCAGCAACACACAGCCCCAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCATCGCCCGACTTGGTACgg                                                                                                              >  4:124269/1‑148 (MQ=255)
                                tCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACggg                                                                                                             <  4:53732/148‑1 (MQ=255)
                                         tCGCATGCCAGCAACACACAGCCACCGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAAc                                                                                                     <  3:78514/147‑1 (MQ=255)
                                            tATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGt                                                                                                 <  3:88574/148‑1 (MQ=255)
                                                    cAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACAAACAGGTTCTCGAcc                                                                                         >  5:122876/1‑148 (MQ=255)
                                                     aaCACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCt                                                                                        <  3:3383/148‑1 (MQ=255)
                                                              ccACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGTCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACt                                                                               >  5:38652/1‑148 (MQ=255)
                                                                       aTGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAACCGTGTTGCAGTAGGCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCcagca                                                                      <  5:96174/148‑1 (MQ=255)
                                                                         ggCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGt                                                                    <  6:100477/148‑1 (MQ=255)
                                                                            ttCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGcc                                                                 >  7:46371/1‑148 (MQ=255)
                                                                            ttCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCATCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGcc                                                                 >  4:27214/1‑148 (MQ=255)
                                                                                          tCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTa                                                   >  5:134389/1‑148 (MQ=255)
                                                                                           cATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAg                                                  >  4:4732/1‑148 (MQ=255)
                                                                                            aTGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAgg                                                 >  2:93136/1‑148 (MQ=255)
                                                                                            aTGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAgg                                                 >  2:58470/1‑148 (MQ=255)
                                                                                             tGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAATGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGc                                                <  2:108835/148‑1 (MQ=255)
                                                                                                    gCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCCCACTg                                         >  3:94131/1‑148 (MQ=255)
                                                                                                    gCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTg                                         >  4:76611/1‑148 (MQ=255)
                                                                                                     cccATGACGACATCGGCACTGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCACGTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGt                                        >  1:26194/1‑148 (MQ=255)
                                                                                                                     cACTGACGAAGAAACGAATACGGTTAATCGTGTTGCAGTAGTCCTCGCCCTACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACaaa                        <  2:75191/148‑1 (MQ=255)
                                                                                                                        tGACGAAGAAACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGc                     <  4:39773/148‑1 (MQ=255)
                                                                                                                              agaaACGAATACGGTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTc               <  2:97143/148‑1 (MQ=255)
                                                                                                                                          ggTTAAACGTGTTGCAGTAGTCCTCTCCCAACTTGGTACGGGTTACCAACAGGTTCTAGACCTGCCCCAACTCGTCCAGCAGTGCATTTCGGCCGCTGTAGGCCACACTGTTGACGCTACAGACAAAGGCCAGTTCGATAtcacgctc   >  4:121744/1‑148 (MQ=255)
                                                                                                                                           gTTAAACGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGATAtcacgctca  >  4:64739/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TGTCTTGCAGACCCAGCGCACGGTTCTCTTCCTCGCCTTGGTCGTATGCCAGCAACACACAGCCACAGGCCATGGCTTCGAAGTTCTTGATCATGTATTCGCCCATGCCGACATCGGCACTGACGAAGAAACGAATACGGTTAAGCGTGTTGCAGTAGTCCTCGCCCGACTTGGTACGGGTTACCAACAGGTTCTCGACCTGCCCCAACTCGTCCAGCAGTGCCTTGCGGCCGCTGTAGGCCACACTGTTGACGCTGCCGACAAAGGCCAGTTCGATATCACGCTCA  >  NC_002947/5621562‑5621848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑