Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,987,130	T→G	I71S (ATC→AGC)	kefB‑III →	glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,987,130	0	T	G	100.0%	81.4 / NA	24	I71S (ATC→AGC)	kefB‑III	glutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/15);  total (9/15)

CCTGGCCAAGCGCCTGCAACTCGGTGCCGTGCTCGGCTACCTGCTCGCCGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAG  >  NC_002947/5986987‑5987270                                                                                                                                                |                                                                                                                                             ccTGGCCAAGCGCCTGCAACTCGGTGCCGTGCTCGGCTACCTGCTCGCCGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCagcatc                                                                                                                                          >  1:149237/1‑148 (MQ=255)      ccAAGCGCCTGCAACTCGGTGCCGTGCTCGGCTACCTGCTCGCCGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCt                                                                                                                                     <  8:101136/148‑1 (MQ=255)        aaGCGCCTGCAACTCGGTGCCGTGCTCGGCTACCTGCTCGCCGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTgg                                                                                                                                   <  4:140135/148‑1 (MQ=255)                        gTGCCGTGCTCGGCTACCTGCTCGCCGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCaaa                                                                                                                     <  3:53781/146‑1 (MQ=255)                             gTGCTCGGCTACCTGCTCGCCGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTtgt                                                                                                              >  4:103668/1‑148 (MQ=255)                                    gCTACCTGCTCGCCGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTg                                                                                                         <  2:146290/146‑1 (MQ=255)                                         cTGCTCGCCGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATgcg                                                                                                    <  2:90047/146‑1 (MQ=255)                                                 cGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGgtg                                                                                          <  1:113189/148‑1 (MQ=255)                                                    tgtgGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGttt                                                                                       <  4:96600/148‑1 (MQ=255)                                                     gtgGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTg                                                                                      <  4:21645/148‑1 (MQ=255)                                                     gtgGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTg                                                                                      <  5:108450/148‑1 (MQ=255)                                                                     aaGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGcc                                                                        >  4:91773/1‑146 (MQ=255)                                                                                cTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGa                                                           >  4:104310/1‑148 (MQ=255)                                                                                cTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGGGTCGGCACTGCCCAAGTGGTGCTGa                                                           <  2:149237/148‑1 (MQ=255)                                                                                  gATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGAcc                                                         >  2:75483/1‑148 (MQ=255)                                                                                               gAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCg                                            >  4:65504/1‑148 (MQ=255)                                                                                               gAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTACTGGTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCg                                            <  7:86520/148‑1 (MQ=255)                                                                                                     gTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCa                                      >  1:147588/1‑148 (MQ=255)                                                                                                      tGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCAt                                     <  3:65504/148‑1 (MQ=255)                                                                                                            acaTTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCt                               >  2:83002/1‑148 (MQ=255)                                                                                                                ttCCGAACTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTc                           <  3:104310/148‑1 (MQ=255)                                                                                                                       cTGGGCGTGGTCTTGCTGCTGTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCa                    <  8:7975/148‑1 (MQ=255)                                                                                                                                         ctgTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACcagcag  >  5:6923/1‑148 (MQ=255)                                                                                                                                          tgTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACcagcag  >  1:114499/1‑147 (MQ=255)                                                                                                                                          tgTTCAGCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACcagca   <  3:61817/146‑1 (MQ=255)                                                                                                                                                |                                                                                                                                             CCTGGCCAAGCGCCTGCAACTCGGTGCCGTGCTCGGCTACCTGCTCGCCGGTGTGGTCATCGGCCCGCAAGCACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAG  >  NC_002947/5986987‑5987270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑