| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 90.9% | 19.3 / ‑6.0 | 11 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (6/4); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 90.9% | 21.3 / NA | 11 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (5/5); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAT > NC_002947/1499347‑1499616 || cGCAGTTAGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgt < 7:63595/148‑1 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGttcgcgg > 3:50630/1‑143 (MQ=255) ccATTCTTAGCCTGAACGAAGCCTTGGCACCACAGCAGTACTGTGGCTGCCAAACGTTGGTAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACG‑GGCTTTGCGGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGAtt < 4:53782/148‑1 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGCAACCCGGGCCGCGGGGCATGGCACC‑CGCGTTGGCCGTGTTCGCGGG‑CCCGCCCGCGCCCACAGCCGGATTGGCCTTAAGCggggcga > 3:28587/1‑144 (MQ=255) agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc < 7:25786/148‑1 (MQ=21) gCCAAACGGTGGCCGCGGGCGGGCCGGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTGGGCCGGGGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc < 3:106263/148‑1 (MQ=25) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACACGCGCCCCCGTGCATTGCAAC‑GGCGTTTCCCGTGTTAGCGGG‑CACGCCCCCGCCCACAGACAGATTGCCATTAAACCGGCCTATCGCGAAACTGAACGCCCCTTTTTTTTTTCCTCg > 8:42723/1‑148 (MQ=12) ggggggggCCGGCTAAACTGGCGCGGGGGGGC‑TGGCCCCGGGTTTTGCGGGTGTG‑GGGGGCCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:143406/141‑1 (MQ=255) aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCGTATCGCTACAGTGACGGCGCGTTTTTTGTTGCCTCGCTAACGAATCCTGCCGCCCTTAGAACg > 3:27585/1‑148 (MQ=25) gTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 2:115448/1‑148 (MQ=38) gTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 2:97939/1‑148 (MQ=38) || CGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAT > NC_002947/1499347‑1499616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |